Lecture 24 – Enzyme kinetics

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Questions and Answers

What is the initial step of the enzyme-catalyzed reaction as described in the Michaelis-Menten model?

  • E + P ⇌ ES
  • E + S ⇌ ES (correct)
  • ES ⇌ E + P
  • ES ⇌ E + P

In the Michaelis-Menten equation, what does Vmax represent?

  • The maximum substrate concentration
  • The maximum rate of the reaction (correct)
  • The rate constant of the reverse reaction
  • The total enzyme concentration

What is represented by the symbol k 1 in enzyme kinetics?

  • The rate constant for product formation
  • The rate constant for the formation of the enzyme-substrate complex (correct)
  • The rate constant for the formation of the enzyme-substrate complex
  • The rate constant for the reverse reaction

Which equation is used to describe the initial rate of an enzyme-catalyzed reaction?

<p>Michaelis-Menten equation (B)</p> Signup and view all the answers

In the context of Michaelis-Menten kinetics, when the substrate concentration is much greater than enzyme concentration, what is assumed?

<p>[S] &gt;&gt; [E] (B)</p> Signup and view all the answers

What is the significance of the steady-state assumption in enzyme kinetics?

<p>It assumes that the concentration of the enzyme-substrate complex remains constant over time. (B)</p> Signup and view all the answers

What does the term k −1 represent in the Michaelis-Menten model?

<p>The rate of dissociation of the enzyme-substrate complex (B)</p> Signup and view all the answers

What is the primary purpose of enzyme kinetics studies?

<p>To understand the rates of enzyme-catalyzed reactions (C)</p> Signup and view all the answers

Which of the following parameters is NOT directly measured in the Michaelis-Menten equation?

<p>Steady-state concentration of the enzyme-substrate complex [ES] (C)</p> Signup and view all the answers

Which of the following describes the relationship between substrate concentration and reaction velocity in the Michaelis-Menten model?

<p>Hyperbolic (B)</p> Signup and view all the answers

In enzyme kinetics, what does the term k2 refer to?

<p>The rate constant for the conversion of the enzyme-substrate complex to product (B)</p> Signup and view all the answers

What is the effect of increasing substrate concentration on the reaction rate when it is at Vmax?

<p>It has no effect. (A)</p> Signup and view all the answers

Who were the scientists that developed the Michaelis-Menten model?

<p>Michaelis and Menten (B)</p> Signup and view all the answers

What is the primary limitation of the Michaelis-Menten model?

<p>It cannot describe all enzymes. (A)</p> Signup and view all the answers

Which parameter indicates how efficiently an enzyme converts substrate into product?

<p>kcat (C)</p> Signup and view all the answers

What is the significance of Km in enzyme kinetics?

<p>It is the substrate concentration at which the reaction rate is half of Vmax (A)</p> Signup and view all the answers

Which of the following statements is true regarding the Michaelis-Menten equation?

<p>It assumes that the enzyme concentration remains constant. (B)</p> Signup and view all the answers

In which year was the Michaelis-Menten equation first proposed?

<p>1913 (B)</p> Signup and view all the answers

What does the term "enzyme-substrate complex" (ES) refer to?

<p>The bound state of the enzyme and substrate (B)</p> Signup and view all the answers

Which of the following factors can influence the velocity of an enzyme-catalyzed reaction?

<p>pH and temperature (A)</p> Signup and view all the answers

What is the effect of temperature on enzyme activity?

<p>It can increase activity up to a certain point, then decreases. (C)</p> Signup and view all the answers

Which of the following best describes the term "steady state" in enzyme kinetics?

<p>The enzyme-substrate complex concentration is constant over time. (C)</p> Signup and view all the answers

In the context of enzyme kinetics, what does a high Km value indicate?

<p>Low affinity of the enzyme for the substrate. (B)</p> Signup and view all the answers

What is the relationship between Vmax and enzyme concentration?

<p>V max is directly proportional to enzyme concentration. (B)</p> Signup and view all the answers

Which of the following is a key assumption of the Michaelis-Menten model?

<p>The formation of the enzyme-substrate complex is a reversible reaction. (B)</p> Signup and view all the answers

Which component of the Michaelis-Menten equation reflects the maximum rate of reaction?

<p>V max (C)</p> Signup and view all the answers

What does the term "velocity" refer to in the context of enzyme kinetics?

<p>The initial rate of reaction (B)</p> Signup and view all the answers

Which of the following models can be used to analyze enzyme kinetics?

<p>All of the above (D)</p> Signup and view all the answers

What impact does a competitive inhibitor have on Km?

<p>It increases Km (B)</p> Signup and view all the answers

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Paris

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