Podcast
Questions and Answers
What is the recommended boiling time for samples before SDS-PAGE?
What is the recommended boiling time for samples before SDS-PAGE?
- 10 minutes (correct)
- 5 minutes
- 20 minutes
- 15 minutes
What is the optimal loading control antibody used in the procedure?
What is the optimal loading control antibody used in the procedure?
- Anti-tubulin antibody [mAbcam 9484]
- Anti-GAPDH antibody [mAbcam 9484] (correct)
- Anti-actin antibody [mAbcam 9484]
- Anti-Bcl-2 antibody [mAbcam 9484]
What type of buffer is recommended for blocking during the procedure?
What type of buffer is recommended for blocking during the procedure?
- 5% milk (correct)
- 2% BSA
- 10% BSA
- 10% milk
What could be a consequence of poor washing during the procedure?
What could be a consequence of poor washing during the procedure?
What is a possible issue when there are air bubbles between the gel and membrane during transfer?
What is a possible issue when there are air bubbles between the gel and membrane during transfer?
Which type of blocking buffer would be more suitable for a different antibody than the Anti-GAPDH used?
Which type of blocking buffer would be more suitable for a different antibody than the Anti-GAPDH used?
What could happen if too much protein or antibody is used in the procedure?
What could happen if too much protein or antibody is used in the procedure?
How can incomplete contact between the gel and membrane during transfer affect the results?
How can incomplete contact between the gel and membrane during transfer affect the results?
What is the purpose of diluting both sample and antibody when too much is used?
What is the purpose of diluting both sample and antibody when too much is used?
Why might different blocking buffers be optimal for different antibodies?
Why might different blocking buffers be optimal for different antibodies?
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