10 Questions
What is the recommended boiling time for samples before SDS-PAGE?
10 minutes
What is the optimal loading control antibody used in the procedure?
Anti-GAPDH antibody [mAbcam 9484]
What type of buffer is recommended for blocking during the procedure?
5% milk
What could be a consequence of poor washing during the procedure?
Incomplete transfer of protein
What is a possible issue when there are air bubbles between the gel and membrane during transfer?
Incomplete contact between gel and membrane
Which type of blocking buffer would be more suitable for a different antibody than the Anti-GAPDH used?
5% BSA
What could happen if too much protein or antibody is used in the procedure?
Decreased signal intensity
How can incomplete contact between the gel and membrane during transfer affect the results?
Increase background noise on the membrane
What is the purpose of diluting both sample and antibody when too much is used?
Enhancing signal strength
Why might different blocking buffers be optimal for different antibodies?
Influencing binding specificity between antibodies and proteins
This quiz provides an overview of Western Blot techniques, including the controls to use in Western Blot, antibody selection, and troubleshooting tips with examples. Topics covered include loading controls for ensuring equal sample loading during SDS-PAGE.
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