Vision and Mission of Faculty of Biotechnology
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Questions and Answers

What is the primary objective of cloning Gene Z cDNA plasmid?

  • To introduce foreign DNA into the plasmid
  • To analyze the genetic content of the plasmid
  • To extract DNA from the plasmid
  • To create a copy of the Gene Z cDNA plasmid (correct)
  • Which strategy would be most suitable for isolating Gene Z cDNA plasmid?

  • Restriction Enzyme Digestion (correct)
  • Gel Electrophoresis
  • Southern Blotting
  • Polymerase Chain Reaction (PCR)
  • What role do ligases play in cloning Gene Z cDNA plasmid?

  • They analyze DNA content
  • They amplify the DNA sequences
  • They cut DNA at specific sequences
  • They join DNA fragments together (correct)
  • Which of the following techniques is crucial for inserting Gene Z cDNA into a plasmid vector?

    <p>Transformation</p> Signup and view all the answers

    What is the purpose of selecting transformed bacterial colonies in cloning Gene Z cDNA plasmid?

    <p>To eliminate non-transformed bacteria</p> Signup and view all the answers

    Which technique could be used to verify the successful integration of Gene Z cDNA into the plasmid vector?

    <p>Sequencing</p> Signup and view all the answers

    In cloning Gene Z cDNA plasmid, what is the purpose of using antibiotic selection?

    <p>To eliminate non-transformed bacteria</p> Signup and view all the answers

    Why is it important to use a selectable marker in the Gene Z cDNA plasmid vector?

    <p>To allow identification of transformed cells</p> Signup and view all the answers

    What is one potential challenge associated with cloning Gene Z cDNA plasmid?

    <p>Inefficient DNA ligation</p> Signup and view all the answers

    During which step of cloning Gene Z cDNA plasmid would you perform bacterial transformation?

    <p>Before ligating DNA fragments</p> Signup and view all the answers

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