10 Questions
What is the primary objective of cloning Gene Z cDNA plasmid?
To create a copy of the Gene Z cDNA plasmid
Which strategy would be most suitable for isolating Gene Z cDNA plasmid?
Restriction Enzyme Digestion
What role do ligases play in cloning Gene Z cDNA plasmid?
They join DNA fragments together
Which of the following techniques is crucial for inserting Gene Z cDNA into a plasmid vector?
Transformation
What is the purpose of selecting transformed bacterial colonies in cloning Gene Z cDNA plasmid?
To eliminate non-transformed bacteria
Which technique could be used to verify the successful integration of Gene Z cDNA into the plasmid vector?
Sequencing
In cloning Gene Z cDNA plasmid, what is the purpose of using antibiotic selection?
To eliminate non-transformed bacteria
Why is it important to use a selectable marker in the Gene Z cDNA plasmid vector?
To allow identification of transformed cells
What is one potential challenge associated with cloning Gene Z cDNA plasmid?
Inefficient DNA ligation
During which step of cloning Gene Z cDNA plasmid would you perform bacterial transformation?
Before ligating DNA fragments
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