Virus Classification: Methods and Characteristics

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Questions and Answers

Which of the listed characteristics is NOT a primary criterion used in the traditional hierarchical virus classification system?

  • Symmetry of the capsid.
  • Nature of the nucleic acid (DNA or RNA).
  • Specific host cell receptor interaction. (correct)
  • Presence or absence of an envelope.

In the Baltimore classification system, what serves as the central point for categorizing viruses?

  • Type of capsid symmetry.
  • Size of the viral genome.
  • Production of mRNA (+ sense RNA). (correct)
  • Presence of a viral envelope.

How does the genomic system classify viruses, especially in recent years?

  • According to the geographical location where the virus was first isolated.
  • Based on the host organism infected.
  • Primarily by the size and shape of the virion.
  • By analyzing the DNA or RNA sequence. (correct)

A newly discovered virus has been found to contain a single-stranded RNA genome and lacks an envelope. According to the traditional hierarchical classification, to what level can this virus be classified with the information provided?

<p>Family level. (D)</p> Signup and view all the answers

If a virus is classified as a positive-sense RNA virus by the Baltimore classification system, what key process must it perform upon entering a host cell?

<p>It can directly use its RNA as mRNA for protein synthesis. (B)</p> Signup and view all the answers

Which of the following characteristics is NOT a primary factor in determining the shape of a virus?

<p>The specific sequence of the viral genome. (C)</p> Signup and view all the answers

If a newly discovered virus has a capsid that appears to have flat sides and edges when viewed under an electron microscope, which shape would be most likely?

<p>Icosahedral. (A)</p> Signup and view all the answers

A researcher identifies a virus that infects bacteria and has a distinct head-tail structure. The head appears icosahedral, and the tail is helical. How should this virus be classified based on its shape?

<p>Complex virus (B)</p> Signup and view all the answers

Which of the following viruses is known to have the largest diameter?

<p>Smallpox virus (C)</p> Signup and view all the answers

A newly discovered virus infects only specific strains of a bacterial species. This is an example of:

<p>Host range specificity (A)</p> Signup and view all the answers

Which of the following factors is MOST crucial for a virus to successfully infect a host cell?

<p>Complementary fit between viral surface proteins and host cell receptors (B)</p> Signup and view all the answers

What is the function of the protein subunits that surround the RNA molecules in a non-enveloped helical virus such as the Tobacco Mosaic Virus (TMV)?

<p>To provide structural support and protection for the viral genome (B)</p> Signup and view all the answers

A virus is found to exclusively target and infect nerve cells in humans. What property of this virus allows it to exhibit such specific targeting?

<p>The complementary interaction between its surface proteins and specific receptors on nerve cells (C)</p> Signup and view all the answers

Which factor primarily dictates a virus's capacity to infect a specific host cell?

<p>The presence of specific receptors on the host cell surface that match viral attachment structures. (D)</p> Signup and view all the answers

A virus with a narrow host range is MOST likely to infect:

<p>a single tissue within a single species. (C)</p> Signup and view all the answers

Before 1966, how were viruses commonly named?

<p>Based on associated diseases, the type of disease they cause, or the sites in the body affected. (A)</p> Signup and view all the answers

The ICTV's (International Committee on Taxonomy of Viruses) classification system is MOST important from which taxonomic level?

<p>Family down to species. (C)</p> Signup and view all the answers

What MUST occur before any translation can occur for a virus with a negative-sense (-) RNA genome?

<p>The negative-sense RNA must be transcribed into a positive-sense (+) RNA. (D)</p> Signup and view all the answers

Which characteristic is NOT a primary factor used in the classification of viruses?

<p>The method of viral transmission between hosts. (A)</p> Signup and view all the answers

Which of the following viruses is MOST likely to exhibit a broad host range?

<p>A virus capable of transmitting from animals to humans but not between humans. (A)</p> Signup and view all the answers

What is the correct suffix for viral families in the hierarchical virus classification?

<p>-viridae (C)</p> Signup and view all the answers

Which characteristic of viral capsomeres is most important for viral classification and identification?

<p>The number of proteins and arrangement. (A)</p> Signup and view all the answers

What is the primary difference between a naked virus and an enveloped virus?

<p>Enveloped viruses have a bilayer membrane outside their capsid, whereas naked viruses do not. (D)</p> Signup and view all the answers

Which component of an enveloped virus is typically derived from the host cell during the budding process?

<p>The lipid membrane. (D)</p> Signup and view all the answers

What is the term for a complete virus particle, including its envelope (if present)?

<p>Virion (B)</p> Signup and view all the answers

What advantage do envelope proteins provide to viruses regarding the host's immune system?

<p>Envelope proteins allow viruses to be hidden from attack by the host’s immune system. (B)</p> Signup and view all the answers

Which environmental condition is least likely to damage enveloped viruses?

<p>Stable pH levels around 7. (D)</p> Signup and view all the answers

Viruses are considered metabolically inert outside of a host cell. What does this imply about their ability to function?

<p>Viruses rely on the host cell's metabolic processes to reproduce. (C)</p> Signup and view all the answers

What is the function of the glycoprotein spikes found on the surface of some viruses?

<p>To enable the virus to attach to specific receptor sites on host cells. (B)</p> Signup and view all the answers

Why is the property of hemagglutination useful in viral identification?

<p>It demonstrates the virus's ability to cause red blood cells to clump together. (C)</p> Signup and view all the answers

A newly discovered microorganism is non-cellular and contains DNA enclosed within a protein coat. It can only replicate inside a host cell. Which of the following is the most likely classification of this microorganism?

<p>Virus (C)</p> Signup and view all the answers

Which of the characteristics listed is NOT a feature of viruses?

<p>Possession of a nucleus (D)</p> Signup and view all the answers

Outside of a host cell, a virus is metabolically inactive. How does the virus become active?

<p>By invading a cell and directing the host machinery to synthesize new virions. (C)</p> Signup and view all the answers

A researcher is studying a virus and finds that its genetic material is single-stranded RNA. Which of the following accurately describes the genetic composition of this virus?

<p>It contains single-stranded RNA. (A)</p> Signup and view all the answers

What is the primary function of the capsid?

<p>To protect the viral nucleic acid. (A)</p> Signup and view all the answers

Which of the following components are found in ALL viruses?

<p>Nucleic acid and capsid (C)</p> Signup and view all the answers

Why are viruses not classified into any of the established biological kingdoms (e.g., Animalia, Plantae, Fungi)?

<p>Viruses do not possess the characteristics of living cells. (B)</p> Signup and view all the answers

Flashcards

Definition of Virus

An infectious agent that can multiply only in living cells.

Characteristics of Viruses

Viruses are small, non-cellular, obligate intracellular parasites.

Virus Components

Viruses contain nucleic acid, capsid, and sometimes an envelope protein.

Nucleic Acid in Viruses

Viruses have either DNA or RNA as their genetic material.

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Capsid Function

The capsid is a protein coat that protects viral nucleic acid and gives shape.

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Obligate Intracellular Parasite

Viruses can only replicate inside the living cells of hosts.

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Metabolic Activity of Viruses

Viruses are metabolically inert outside of host cells.

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Virus Replication Process

Once inside a host cell, viruses direct its machinery to create new virus particles.

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Capsomeres

Protein subunits that compose the capsid of a virus.

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Naked Virus

A virus that consists only of capsid proteins without an envelope.

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Enveloped Virus

A virus that has a lipid bilayer membrane outside the capsid.

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Virion

A complete virus particle, including its capsid and envelope.

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Nucleocapsid

The combination of the viral genome and the capsid within a virion.

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Spikes

Glycoprotein projections from the viral envelope that help attach viruses to host cells.

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Hemagglutination

The clumping of red blood cells due to viral spikes binding to them.

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Viral Size

Viruses are measured in nanometers, often too small for light microscopes.

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Smallest Virus

The poliovirus measures 28 nm, similar in size to a ribosome.

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Hierarchical Classification

A system grouping viruses by phylum, class, order, family, genus, species, and strain.

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Largest Virus

The smallpox virus is the largest, measuring 200 nm in diameter.

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Baltimore Classification System

Classifies viruses based on their method of mRNA production, focusing on RNA types.

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Helical Virus Shape

Helical viruses have a spiral shape, often with RNA surrounded by protein subunits.

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Viral Characteristics

Classified based on nucleic acid type, capsid symmetry, envelope presence, and size.

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Polyhedral Virus Shape

Polyhedral viruses are shaped like geometric figures, with flat sides; e.g., adenovirus.

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Genomic Classification

Uses DNA/RNA sequences to organize viruses more accurately within families.

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Complex Virus Structure

Complex viruses, like bacteriophage, have an isocohedral head and helical tail.

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mRNA Sense

Viruses need to produce mRNA; (+) sense is the coding strand, whereas (-) sense is complementary.

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Animal Viruses

Animal viruses infect animals, including rabies, polio, and influenza.

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Plant Viruses

Plant viruses like TMV can infect plants, causing various diseases.

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Host Range Specificity

Viruses have a limited host range, attacking specific cells and tissues.

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Broad host range

Viruses that can infect multiple host species, such as swine flu and rabies.

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Narrow host range

Viruses that infect a single species or tissue type, such as adenovirus and HIV.

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Virus specificity

Determined by specific receptors on host cells and viral attachment structures.

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Classification of viruses

Based mainly on host type, nucleic acid type, polarity of RNA, and coat morphology.

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Nucleic acid types

Viruses can have dsDNA, dsRNA, ssDNA, or ssRNA as their genetic material.

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Virus coat morphology

Describes whether a virus has an envelope or not.

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Hierarchical virus classification

Classification important mainly from the family level onward, with families ending in 'viridae'.

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Virus nomenclature history

First 60 years were named based on diseases or the first locations discovered.

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Study Notes

Virus Introduction

  • Viruses are infectious agents.
  • They are very small, requiring a microscope to see.
  • They are not part of any kingdom (not plants, animals, fungi, protists, or bacteria).
  • They are obligate intracellular parasites; they can only replicate within a host cell, relying on host metabolic processes.
  • Viral diseases result from the interaction between the virus and host cell.

Virus Characteristics

  • Viruses are too small to be seen with a light microscope.
  • They are non-cellular, lacking a nucleus, organelles, cytoplasm, or cell membrane.
  • Viruses are obligate intracellular parasites, meaning they multiply only inside living cells.
  • They cannot divide or live outside a host cell.
  • Outside a host cell (extracellular), viral metabolism is inactive, and biosynthetic functions are impossible.
  • Once inside a cell, they direct host machinery to produce new complete viral particles (virions), which complete the replication process.

Virus Components

  • Viruses contain nucleic acid (either DNA or RNA).
  • The capsid, composed of capsomeres, provides a protective protein coat.
  • Some viruses have an envelope, a lipid-protein membrane outside the capsid.
    • The envelope is derived from host cell membranes, and the material may include carbohydrates
  • The nucleocapsid contains the viral genome along with the capsid.

Viral Nucleic Acid

  • Viruses contain either DNA or RNA, but not both.
    • Nucleic acid can be double-stranded (DS), single-stranded (SS), linear, circular, or segmented.

Viral Capsids

  • The capsid (or capsids) is the surrounding protein coat around the nucleic acid.
  • Determine the shape of the virus.
  • Contains protein subunits known as capsomeres.
  • The number and arrangement of capsomeres are important in classifying and identifying viruses.

Viral Envelopes

  • Some viruses have a membrane called an envelope surrounding the capsid.
    • Consisting of lipids, proteins, and carbohydrates
  • Enveloped viruses are often derived from host cell membranes as they bud from or detach from the host cell.
  • Enveloped viruses can lose their envelope under harsh conditions (e.g., high temperature, freezing/thawing, certain chemicals).

Viral Shapes and Sizes

  • Viruses exhibit various shapes (helical, polyhedral, complex).
  • Sizes fluctuate among different viruses.
    • Measured in nanometers (nm).
    • Poliovirus (28 nm) is the smallest virus shown; smallpox viruses are large (200 nm).

Host Range and Specificity

  • Viruses have a limited host range, attacking only one or a few host types or cell types.
  • Viruses show specificity for cells/tissue—a complementary fit between external viral proteins and host cell surface proteins.
    • This specificity impacts the host cells infected.
  • Broad-range viruses can infect various host types (e.g., swine flu and rabies), while narrow-range viruses are limited to a single tissue type in a single species (e.g., HIV, phages on E. coli).

Virus Classification

  • Viruses are classified based on their genetic material, morphology of their capsid, presence or absence of an envelope, and their host range.
  • The ICTV (International Committee on Taxonomy of Viruses) maintains a systematic system for classifying viruses.
  • Historically, method classification has been developed using the following:
    • type of disease caused;
    • site of the first virus isolation;
    • scientific discoverer of the virus;
    • perceived method of contraction

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