Vector-Borne Pathogens and Disease Development
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Questions and Answers

  1. What are the factors that affect the spatial spread of vector-borne diseases?

Factors affecting the spatial spread of vector-borne diseases include vector movement and aggregation, wind direction affecting aphid dispersal, the presence of alternate hosts around the fields, and disease gradients estimated from observed frequencies at different distances from the source plant.

  1. What is the 'multiple infection transformation' used for in estimating the number of inoculations?

The 'multiple infection transformation' is used to estimate the number of inoculations by transforming the observed disease frequencies close to the source plant. It is represented by the equation $ln[1/(1-x)]$, where x is the proportion of diseased plants or diseased area.

  1. What control methods are recommended for vector-borne diseases?

Control methods for vector-borne diseases depend on the pathogen, vector, and host. Prevention of vector landing on the crop, host plant resistance, reflective mulches, and a combination of different control tactics are recommended. Spraying of the vector may not always be effective, especially for non-persistent vectors.

  1. How does the spatial spread of black currant reversion virus transmitted by aphids relate to the 'multiple infection transformation'?

<p>The spatial spread of black currant reversion virus transmitted by aphids is observed and then transformed using the 'multiple infection transformation' to estimate the number of inoculations close to the source. The solid line represents the observed spread, while the dotted line represents the transformed spread.</p> Signup and view all the answers

  1. What is the effect of roguing of infected plants in controlling vector-borne diseases?

<p>Roguing of infected plants may be effective in controlling vector-borne diseases, especially when the incubation period is short. It is dependent on the stage of disease development of the rogued plants, and roguing of infectious symptomatic and asymptomatic plants can be effective.</p> Signup and view all the answers

  1. How does the incidence of tomato yellow leaf curl change when a barrier with insecticide is used compared to a barrier without insecticide?

<p>The incidence of tomato yellow leaf curl, transmitted by Bemisia tabaci whiteflies, is lower when a barrier with insecticide is used compared to a barrier without insecticide, as shown by the solid line with barrier being below the dotted line without barrier.</p> Signup and view all the answers

  1. What is the significance of roguing of symptomatic plants on the incidence of cassava mosaic virus, transmitted by whiteflies over time?

<p>Roguing of symptomatic plants has a significant impact on the incidence of cassava mosaic virus, as indicated by the difference in incidence over time between the group with no roguing (closed dots) and the group with roguing (open dots).</p> Signup and view all the answers

  1. Why is a combination of different control tactics needed to reduce epidemic development of vector-borne diseases?

<p>A combination of different control tactics is needed to reduce epidemic development of vector-borne diseases because control methods depend on various factors such as the specific pathogen, vector, host, and the presence of other means of dispersal. Additionally, spraying of the vector may not always be effective, and prevention of vector landing on the crop, host plant resistance, and reflective mulches are among the recommended control methods.</p> Signup and view all the answers

Explain the influence of pathogen population size on disease development.

<p>Pathogen population size, or the number of propagules, can influence disease development by affecting the likelihood of successful transmission to a host plant and the establishment of infection.</p> Signup and view all the answers

Describe the vector relationships that can influence disease development.

<p>Vector relationships, including specificity and persistence, play a crucial role in disease development as they determine the ability of the pathogen to be transmitted and maintained within the vector population.</p> Signup and view all the answers

What are the key characteristics of pathogens that can influence disease development?

<p>The level of virulence, replication, and movement inside plants, as well as ecological factors such as soilborne or foliar survival, are key characteristics of pathogens that can significantly influence disease development.</p> Signup and view all the answers

How are plant pathogenic fungi transmitted to different parts of plants?

<p>Spores of plant pathogenic fungi can be transmitted to flowers by thrips and various pollinators, or to the bark and wood of trees by bark boring beetles.</p> Signup and view all the answers

How are viruses and other pathogens quantified in vectors?

<p>For many plant virus diseases, the epidemiologically important propagule is often the vector. The same is true for phloem-limited bacteria or mollicutes.</p> Signup and view all the answers

What are some examples of insect vectors that transmit viruses, bacteria, and mollicutes?

<p>Insects belonging to the order Homoptera, such as aphids, whiteflies, psyllids, planthoppers, and leafhoppers, are the main insect vectors that transmit viruses, bacteria, and mollicutes.</p> Signup and view all the answers

Explain the importance of trap design in quantifying insect vectors.

<p>Trap design affects catch by influencing the effectiveness of the trap in capturing specific insect species, with factors such as size, color, height, location, and position playing crucial roles in the quantification process.</p> Signup and view all the answers

What are the different methods used for identifying and quantifying viruses in plants or vectors?

<p>Real-time quantitative PCR methods and amplicon sequence specific and non-specific methods are utilized for identifying and quantifying viruses in plants or vectors.</p> Signup and view all the answers

Describe the concept of vector relationships and its significance in epidemic development.

<p>Vector relationships encompass specificity and persistence, with specificity referring to the transmission by particular species or non-specifically, and persistence affecting epidemic development based on non-persistence, semi-persistence, circulative persistence, or circulative persistence and propagative traits.</p> Signup and view all the answers

Discuss the five steps involved in vector transmission and their relevance in disease spread.

<p>The five steps in vector transmission include acquisition, movement inside the vector, multiplication, inoculation, and movement/replication in the host plant, and they play a crucial role in disease spread and epidemic development.</p> Signup and view all the answers

Explain the factors that affect virus transmission and epidemic development.

<p>Factors affecting transmission and epidemic development include the proportion of infected source plants, pathogen content, vector density, vector aggregation, vector movement, and the presence of alternate hosts.</p> Signup and view all the answers

How can plant virus infection impact vector behavior and disease transmission?

<p>Plant virus infection can affect vector multiplication rate and may also lead to vectors being more attracted to diseased plants, indicating co-evolution between the virus and the vectors.</p> Signup and view all the answers

Study Notes

  • Insect vectors used for quantification: Landing and impaction traps (water pan trap, yellow sticky traps), vertical net, suction traps
  • Yellow sticky traps more effective for Homoptera than water pans
  • Trap design affects catch: size, color, height, location, position
  • Suction traps useful for measuring vector density, but expensive and labor-intensive
  • Real-time quantitative PCR methods for identifying and quantifying viruses in plants or vectors
  • Amplicon sequence non-specific methods: SYBR Green I, fluorescence proportional to total dsDNA
  • Amplicon sequence specific methods: TaqMan, Molecular beacons, Scorpion PCR, probe labeled with a donor fluorophore and acceptor dye
  • Vector relationships: specificity (transmission by particular species or non-specifically), persistence (non-persistent, semi-persistent, circulative persistent, or circulative persistent and propagative)
  • Persistence in the vector affects epidemic development
  • Five steps in vector transmission: acquisition, movement inside the vector, multiplication, inoculation, and movement/replication in the host plant
  • Factors affecting transmission and epidemic development: proportion of infected source plants, pathogen content, vector density, vector aggregation, vector movement, presence of alternate hosts
  • Modeled virus transmission: acquisition and inoculation over time, disease incidence over time for non-persistent, semi-persistent, circulative persistent, and propagative persistent viruses
  • Multiplication and movement of pathogens inside host plants affect epidemic development
  • Plant virus infection can affect vector multiplication rate
  • Vectors can be more attracted to diseased plants, indicating co-evolution.

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Explore the influence of vector-borne pathogens, including viruses, bacteria, and fungi, on disease development. Learn about the diverse range of vectors involved in transmitting these pathogens, from insects to higher animals.

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