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Questions and Answers
What is the primary difference between a single beam and a double beam spectrophotometer?
What is the primary difference between a single beam and a double beam spectrophotometer?
- The wavelength of light used
- The type of sample being analyzed
- The number of light beams used (correct)
- The intensity of the light beam
What is the purpose of a beam splitter in a double beam spectrophotometer?
What is the purpose of a beam splitter in a double beam spectrophotometer?
- To filter out unwanted wavelengths
- To increase the intensity of the light beam
- To reduce the noise in the signal
- To split the light beam into two separate beams (correct)
What is the advantage of using a double beam spectrophotometer over a single beam spectrophotometer?
What is the advantage of using a double beam spectrophotometer over a single beam spectrophotometer?
- Increased accuracy (correct)
- Faster measurement times
- Reduced cost
- Improved sensitivity
What is the application of UV-VIS spectrophotometry in quantitative analysis of a single component?
What is the application of UV-VIS spectrophotometry in quantitative analysis of a single component?
What is the purpose of constructing a calibration curve in UV-VIS spectrophotometry?
What is the purpose of constructing a calibration curve in UV-VIS spectrophotometry?
What is the relationship between absorbance and concentration of a sample according to Beer-Lambert's law?
What is the relationship between absorbance and concentration of a sample according to Beer-Lambert's law?
What is the requirement for the sample and blank in a single beam spectrophotometer?
What is the requirement for the sample and blank in a single beam spectrophotometer?
What is the advantage of using a single beam spectrophotometer over a double beam spectrophotometer?
What is the advantage of using a single beam spectrophotometer over a double beam spectrophotometer?
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Study Notes
Complexation Measurement
- The composition of a stable complex can be determined by two techniques: the mole-ratio method and the method of continuous variations.
UV-VIS Spectrophotometry
- Two types of spectrophotometers: Single Beam Spectrometer and Double Beam Spectrometer.
- Single Beam Spectrometer:
- Uses one light beam for the sample or the blank.
- Less accurate.
- The sample and blank must be placed alternately in the light path.
- Does not contain a beam splitter.
- Double Beam Spectrometer:
- Uses two light beams: one for the sample and the other for the reference (blank).
- More accurate.
- The sample and blank are placed at the same time in the light path.
- Contains a beam splitter.
Applications of UV-VIS Spectrophotometry
- Quantitative analysis of a single component:
- A calibration curve (Beer-Lambert's) is constructed by determining the absorbance values of a series of solutions at the selected λmax.
- Useful for estimating the maximum wavelength at which the quantitative measurement should be done.
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