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Questions and Answers
What type of spectroscopy does a UV-vis spectrophotometer measure?
What type of spectroscopy does a UV-vis spectrophotometer measure?
What is the main requirement for a sample to be suitable for UV-Vis spectroscopy?
What is the main requirement for a sample to be suitable for UV-Vis spectroscopy?
What parameter of interest is measured in UV-Vis spectrophotometry besides the wavelength of measurement?
What parameter of interest is measured in UV-Vis spectrophotometry besides the wavelength of measurement?
What type of energy do most molecules and ions absorb in the ultraviolet or visible range?
What type of energy do most molecules and ions absorb in the ultraviolet or visible range?
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What does the amount of light absorbed by a sample in a UV-vis spectrophotometer indicate?
What does the amount of light absorbed by a sample in a UV-vis spectrophotometer indicate?
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What is the main function of a UV-vis spectrophotometer?
What is the main function of a UV-vis spectrophotometer?
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What does the term 'chromophore' refer to in the context of UV-Vis spectrophotometry?
What does the term 'chromophore' refer to in the context of UV-Vis spectrophotometry?
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What is the relationship between absorbance and the concentration of an absorbing compound in a sample?
What is the relationship between absorbance and the concentration of an absorbing compound in a sample?
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What is the complementary spectroscopy technique to absorption spectroscopy in UV-Vis spectrophotometry?
What is the complementary spectroscopy technique to absorption spectroscopy in UV-Vis spectrophotometry?
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What is the function of an ultraviolet–visible (UV-VIS) spectrophotometer?
What is the function of an ultraviolet–visible (UV-VIS) spectrophotometer?
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Study Notes
UV-Vis Spectrophotometry
- A UV-vis spectrophotometer measures the absorption of light in the ultraviolet and visible regions of the electromagnetic spectrum.
- For a sample to be suitable for UV-Vis spectroscopy, it must be able to absorb light in the UV-Vis range, typically having a chromophore, a group of atoms that absorbs light.
- In UV-Vis spectrophotometry, besides the wavelength of measurement, the parameter of interest measured is absorbance, which is a measure of the amount of light absorbed by a sample.
- Most molecules and ions absorb electromagnetic energy in the ultraviolet or visible range, resulting in electronic transitions.
- The amount of light absorbed by a sample in a UV-vis spectrophotometer indicates the presence and concentration of a particular absorbing compound.
- The main function of a UV-vis spectrophotometer is to measure the absorption of light by a sample as a function of wavelength, providing information about the sample's molecular structure.
- A chromophore refers to a group of atoms in a molecule responsible for absorbing light in the UV-Vis range, resulting in a specific absorption spectrum.
- There is a direct relationship between absorbance and the concentration of an absorbing compound in a sample, as described by the Beer-Lambert law.
- Emission spectroscopy is the complementary spectroscopy technique to absorption spectroscopy in UV-Vis spectrophotometry, measuring the emitted light instead of absorbed light.
- The function of an ultraviolet-visible (UV-VIS) spectrophotometer is to quantify the interaction between matter and electromagnetic radiation in the UV-Vis range, providing information about the sample's molecular structure and composition.
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Description
Test your knowledge of UV spectroscopy with this quiz! Explore the principles, applications, and instrumentation of UV-VIS spectrophotometry. See how well you understand this widely used analytical technique.
