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Questions and Answers
What type of spectroscopy does a UV-vis spectrophotometer measure?
What is the main requirement for a sample to be suitable for UV-Vis spectroscopy?
What parameter of interest is measured in UV-Vis spectrophotometry besides the wavelength of measurement?
What type of energy do most molecules and ions absorb in the ultraviolet or visible range?
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What does the amount of light absorbed by a sample in a UV-vis spectrophotometer indicate?
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What is the main function of a UV-vis spectrophotometer?
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What does the term 'chromophore' refer to in the context of UV-Vis spectrophotometry?
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What is the relationship between absorbance and the concentration of an absorbing compound in a sample?
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What is the complementary spectroscopy technique to absorption spectroscopy in UV-Vis spectrophotometry?
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What is the function of an ultraviolet–visible (UV-VIS) spectrophotometer?
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Study Notes
UV-Vis Spectrophotometry
- A UV-vis spectrophotometer measures the absorption of light in the ultraviolet and visible regions of the electromagnetic spectrum.
- For a sample to be suitable for UV-Vis spectroscopy, it must be able to absorb light in the UV-Vis range, typically having a chromophore, a group of atoms that absorbs light.
- In UV-Vis spectrophotometry, besides the wavelength of measurement, the parameter of interest measured is absorbance, which is a measure of the amount of light absorbed by a sample.
- Most molecules and ions absorb electromagnetic energy in the ultraviolet or visible range, resulting in electronic transitions.
- The amount of light absorbed by a sample in a UV-vis spectrophotometer indicates the presence and concentration of a particular absorbing compound.
- The main function of a UV-vis spectrophotometer is to measure the absorption of light by a sample as a function of wavelength, providing information about the sample's molecular structure.
- A chromophore refers to a group of atoms in a molecule responsible for absorbing light in the UV-Vis range, resulting in a specific absorption spectrum.
- There is a direct relationship between absorbance and the concentration of an absorbing compound in a sample, as described by the Beer-Lambert law.
- Emission spectroscopy is the complementary spectroscopy technique to absorption spectroscopy in UV-Vis spectrophotometry, measuring the emitted light instead of absorbed light.
- The function of an ultraviolet-visible (UV-VIS) spectrophotometer is to quantify the interaction between matter and electromagnetic radiation in the UV-Vis range, providing information about the sample's molecular structure and composition.
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Description
Test your knowledge of UV spectroscopy with this quiz! Explore the principles, applications, and instrumentation of UV-VIS spectrophotometry. See how well you understand this widely used analytical technique.
