Podcast
Questions and Answers
What is the primary reaction used in the Multistix urobilinogen test?
What is the primary reaction used in the Multistix urobilinogen test?
- Chromatographic separation
- Ehrlich aldehyde reaction (correct)
- Enzymatic colorimetric reaction
- Azo dye formation from a diazonium compound
What color change indicates a positive result for urobilinogen in the Multistix test?
What color change indicates a positive result for urobilinogen in the Multistix test?
- Light yellow to deep blue
- Colorless to pink
- Green to yellow
- Reddish brown (correct)
Which of the following substances can also be detected by the Ehrlich reagent apart from urobilinogen?
Which of the following substances can also be detected by the Ehrlich reagent apart from urobilinogen?
- Indole (correct)
- Creatinine
- Porphobilinogen
- Urea
What is the range of normal urobilinogen values indicated by the Multistix test?
What is the range of normal urobilinogen values indicated by the Multistix test?
How quickly should results be read in the Chemstrip urobilinogen test?
How quickly should results be read in the Chemstrip urobilinogen test?
Which method is NOT a reliable detection method for porphobilinogen?
Which method is NOT a reliable detection method for porphobilinogen?
What is the chemical compound used in Chemstrip for detecting urobilinogen?
What is the chemical compound used in Chemstrip for detecting urobilinogen?
What is a common limitation of the Ehrlich reaction in urobilinogen testing?
What is a common limitation of the Ehrlich reaction in urobilinogen testing?
Flashcards
Ehrlich aldehyde reaction
Ehrlich aldehyde reaction
A chemical reaction that involves the formation of a colored product when a compound containing an aldehyde group reacts with a reagent.
Ehrlich reagent
Ehrlich reagent
A reagent that produces a colored product when it reacts with urobilinogen in a urine sample.
Urobilinogen
Urobilinogen
A substance that produces urine with a red-brown hue and is a sign of bilirubin breakdown.
Reagent strip test for urobilinogen
Reagent strip test for urobilinogen
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Porphobilinogen
Porphobilinogen
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Chemstrip urobilinogen test
Chemstrip urobilinogen test
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Diazotization
Diazotization
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4-methoxybenzene-diazonium-tetrafluoroborate
4-methoxybenzene-diazonium-tetrafluoroborate
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Study Notes
Reagent Strip Methods for Urobilinogen Testing
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Mechanism: Urobilinogen testing relies on either the Ehrlich aldehyde reaction or the formation of a red azo dye from a diazonium compound.
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Multistix Method: Multistix uses the Ehrlich aldehyde reaction. The test area is coated with an acid buffer and p-dimethylaminobenzaldehyde. This produces a reddish-brown color with urobilinogen. Color intensity correlates to concentration. Normal values are 0.2-1 mg/dL.
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Multistix Limitations: This method isn't specific to urobilinogen. It detects other substances that react with Ehrlich's reagent, including porphobilinogen, p-aminosalicylic acid metabolites, sulfonamides, procaine, 5-hydroxyindoleacetic acid, indole, and methyldopa. It's unreliable for detecting porphobilinogen.
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Chemstrip Method: Chemstrip uses a diazonium compound, 4-methoxybenzene-diazonium-tetrafluoroborate. This compound reacts with urobilinogen in an acidic environment to create a red azo dye. Results are visible within 10-30 seconds.
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Chemstrip Sensitivity: The Chemstrip method can detect approximately 0.4 mg/dL urobilinogen.
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