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Questions and Answers
What is the primary reaction used in Multistix for urobilinogen testing?
What is the primary reaction used in Multistix for urobilinogen testing?
- Ehrlich aldehyde reaction (correct)
- Formation of chloroform
- Hydrolysis of urobilinogen
- Oxidation of urobilinogen
What color variation indicates the presence of urobilinogen in Multistix testing?
What color variation indicates the presence of urobilinogen in Multistix testing?
- Colorless to purple
- Light green to dark blue
- Clear to pinkish
- Light yellow to shades of red-brown (correct)
What substances can be detected by the Ehrlich reagent aside from urobilinogen?
What substances can be detected by the Ehrlich reagent aside from urobilinogen?
- Only indole and indole derivatives
- Only p-aminosalicylic acid
- Only porphobilinogen
- A range of metabolites including sulfonamides (correct)
How is urobilinogen detected using the Chemstrip method?
How is urobilinogen detected using the Chemstrip method?
What is the normal range for urobilinogen values in deciliters according to Multistix testing?
What is the normal range for urobilinogen values in deciliters according to Multistix testing?
What is a limitation of the multistix method for detecting porphobilinogen?
What is a limitation of the multistix method for detecting porphobilinogen?
What is the primary indicator of urobilinogen presence in the Chemstrip test?
What is the primary indicator of urobilinogen presence in the Chemstrip test?
What is the detection limit for urobilinogen in the Chemstrip test?
What is the detection limit for urobilinogen in the Chemstrip test?
Which of the following methods primarily relies on the formation of an azo dye?
Which of the following methods primarily relies on the formation of an azo dye?
What compound is used in Multistix testing to produce a reddish brown color?
What compound is used in Multistix testing to produce a reddish brown color?
Flashcards
Ehrlich aldehyde reaction
Ehrlich aldehyde reaction
A test used to detect urobilinogen in urine, based on a chemical reaction that produces a reddish-brown color.
Multistix urobilinogen test
Multistix urobilinogen test
A reagent strip test that uses the Ehrlich aldehyde reaction to detect urobilinogen in urine.
Ehrlich reagent cross-reactant
Ehrlich reagent cross-reactant
A substance that reacts with the Ehrlich reagent, leading to false-positive results in the urobilinogen test.
Chemstrip urobilinogen test
Chemstrip urobilinogen test
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4-methoxybenzene-diazonium-tetrafluoroborate
4-methoxybenzene-diazonium-tetrafluoroborate
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Diazonium coupling reaction
Diazonium coupling reaction
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Normal urobilinogen range
Normal urobilinogen range
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False-positive substances for urobilinogen
False-positive substances for urobilinogen
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Porphobilinogen
Porphobilinogen
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Reagent strip method for urobilinogen
Reagent strip method for urobilinogen
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Study Notes
Reagent Strip Methods for Urobilinogen Testing
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Principle: Urobilinogen testing relies on either the Ehrlich aldehyde reaction or a diazonium compound reaction to form a colored product.
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Multistix Method: Multistix uses the Ehrlich aldehyde reaction. Its test area contains an acid buffer and p-dimethylaminobenzaldehyde. Urobilinogen reacts with this reagent, producing a reddish-brown color. Normal values range from 0.2 to 1 mg/dL.
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Color Interpretation: Color varies from light yellow to shades of red-brown.
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Limitations: The Multistix method isn't specific to urobilinogen. It detects substances that react with the Ehrlich reagent, including porphobilinogen, certain drug metabolites (e.g., p-aminosalicylic acid, sulfonamides), procaine, 5-hydroxyindoleacetic acid, indole, and methyldopa. This method is unreliable for detecting porphobilinogen.
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Chemstrip Method: Chemstrip uses a diazonium compound (4-methoxybenzene-diazonium-tetrafluoroborate) to detect urobilinogen. This compound reacts with urobilinogen in an acidic environment to form a red azo dye.
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Chemstrip Results: Results are read quickly (10-30 seconds). The test detects approximately 0.4 mg/dL of urobilinogen.
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