Podcast
Questions and Answers
What method does Multistix use for urobilinogen testing?
What method does Multistix use for urobilinogen testing?
- Oxidation-reduction reaction
- Formation of a blue azo dye
- Ehrlich aldehyde reaction (correct)
- Spectrophotometric analysis
What color variation indicates the presence of urobilinogen in Multistix testing?
What color variation indicates the presence of urobilinogen in Multistix testing?
- White to dark brown
- Light yellow to shades of red-brown (correct)
- Green to blue
- Pink to purple
Which of the following substances is not reliably detected by the Multistix method?
Which of the following substances is not reliably detected by the Multistix method?
- p-aminosalicylic acid metabolites
- Porphobilinogen (correct)
- Indole
- Procaine
What is the normal range of urobilinogen levels detected by the Multistix method?
What is the normal range of urobilinogen levels detected by the Multistix method?
What compound does the Chemstrip method use to couple with urobilinogen?
What compound does the Chemstrip method use to couple with urobilinogen?
How long does it typically take to read the results from a Chemstrip urobilinogen test?
How long does it typically take to read the results from a Chemstrip urobilinogen test?
Which of the following is a reaction based on the Chemstrip method?
Which of the following is a reaction based on the Chemstrip method?
What is the primary purpose of the acid buffer solution in the Multistix method?
What is the primary purpose of the acid buffer solution in the Multistix method?
Which of the following statements about the Multistix test method is accurate?
Which of the following statements about the Multistix test method is accurate?
What is the possible detection limit of the Chemstrip urobilinogen test?
What is the possible detection limit of the Chemstrip urobilinogen test?
Flashcards
Reagent Strip Testing
Reagent Strip Testing
The process of determining the concentration or presence of a substance in a sample, often using a chemical reaction.
Ehrlich Aldehyde Reaction
Ehrlich Aldehyde Reaction
A chemical reaction used in reagent strip testing for urobilinogen. It involves reacting a sample with p-dimethylaminobenzaldehyde, producing a reddish-brown color if urobilinogen is present.
Azo Dye Formation
Azo Dye Formation
A method used in reagent strip testing involving a chemical reaction that forms a red azo dye. The dye is produced when a diazonium compound reacts with a substance in the sample.
Multistix
Multistix
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Chemstrip Urobilinogen Test
Chemstrip Urobilinogen Test
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Urobilinogen
Urobilinogen
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Porphobilinogen
Porphobilinogen
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Ehrlich Reagent Interference
Ehrlich Reagent Interference
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Reagent Strip Testing for Urobilinogen
Reagent Strip Testing for Urobilinogen
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Normal Urobilinogen Values
Normal Urobilinogen Values
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Study Notes
Reagent Strip Methods for Urobilinogen
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Principle: Urobilinogen testing relies on the Ehrlich aldehyde reaction or the formation of a red azo dye from a diazonium compound.
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Multistix Method: Multistix uses the Ehrlich aldehyde reaction. A test area is impregnated with an acidic buffer and p-dimethylaminobenzaldehyde, which reacts with urobilinogen to produce a reddish-brown colored result. Normal values range from 0.2-1 mg/dL.
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Multistix Limitations: This method is not specific to urobilinogen. It will also detect other substances that react with the Ehrlich reagent, including porphobilinogen, p-aminosalicylic acid metabolites, sulfonamides, procaine, 5-hydroxyindoleacetic acid, indole, and methyldopa. It's unreliable for detecting porphobilinogen.
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Chemstrip Method: The Chemstrip method utilizes 4-methoxybenzene-diazonium-tetrafluoroborate, which couples with urobilinogen in an acidic environment to generate a red azo dye.
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Chemstrip Results: Results are read quickly, typically within 10–30 seconds. The test has a detection limit of approximately 0.4 mg/dL.
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