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Questions and Answers
What information is essential to include on urine specimen labels immediately after collection?
What information is essential to include on urine specimen labels immediately after collection?
Which of the following is the primarily purpose of including the date and time of urine collection on requisition forms?
Which of the following is the primarily purpose of including the date and time of urine collection on requisition forms?
In a urinalysis laboratory, what is the importance of conical bottom tubes?
In a urinalysis laboratory, what is the importance of conical bottom tubes?
Why is it important to state specific information on specimen collection and handling at the beginning of each procedure listed in the manual?
Why is it important to state specific information on specimen collection and handling at the beginning of each procedure listed in the manual?
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What type of urine collection container is most appropriate for automated reagent strip testing?
What type of urine collection container is most appropriate for automated reagent strip testing?
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What is the primary advantage of using a collection container that holds 12 mL of specimen for microscopic analysis?
What is the primary advantage of using a collection container that holds 12 mL of specimen for microscopic analysis?
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For which type of urine analysis is it most crucial to use individually packaged sterile containers with secure closures?
For which type of urine analysis is it most crucial to use individually packaged sterile containers with secure closures?
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What is the recommended action to preserve a urine specimen that cannot be tested within 2 hours of collection?
What is the recommended action to preserve a urine specimen that cannot be tested within 2 hours of collection?
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What is the primary function of the BD Vacutainer Urine Transfer Straw?
What is the primary function of the BD Vacutainer Urine Transfer Straw?
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What is the most significant consequence of allowing a urine specimen to remain unpreserved at room temperature for more than 2 hours?
What is the most significant consequence of allowing a urine specimen to remain unpreserved at room temperature for more than 2 hours?
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Which urine preservative is LEAST suitable when performing chemical tests for glucose, blood, and leukocyte esterase?
Which urine preservative is LEAST suitable when performing chemical tests for glucose, blood, and leukocyte esterase?
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A urine specimen requires preservation for drug analysis. Which preservative is MOST appropriate?
A urine specimen requires preservation for drug analysis. Which preservative is MOST appropriate?
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Which of the following is a disadvantage of using hydrochloric acid as a preservative for urine samples?
Which of the following is a disadvantage of using hydrochloric acid as a preservative for urine samples?
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Which type of urine specimen is MOST susceptible to showing erroneous results due to dietary intake or physical activity right before collection?
Which type of urine specimen is MOST susceptible to showing erroneous results due to dietary intake or physical activity right before collection?
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For general urine screening, which specimen type is considered the ideal choice?
For general urine screening, which specimen type is considered the ideal choice?
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Which of the following is a key advantage of using formalin as a urine preservative?
Which of the following is a key advantage of using formalin as a urine preservative?
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What is a primary advantage of using hydrochloric acid or acetic acid as a urine preservative?
What is a primary advantage of using hydrochloric acid or acetic acid as a urine preservative?
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A physician orders a urine culture transport. Based on the materials, which would be best?
A physician orders a urine culture transport. Based on the materials, which would be best?
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What change occurs in the urine's odor when it is left unpreserved for a long time?
What change occurs in the urine's odor when it is left unpreserved for a long time?
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Which of the following best describes the effect of long-standing, unpreserved urine on the concentration of glucose?
Which of the following best describes the effect of long-standing, unpreserved urine on the concentration of glucose?
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Why does the pH of an unpreserved urine sample typically increase over time?
Why does the pH of an unpreserved urine sample typically increase over time?
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How does exposure to light affect the bilirubin levels in an unpreserved urine sample?
How does exposure to light affect the bilirubin levels in an unpreserved urine sample?
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What is the primary reason for refrigerating urine specimens?
What is the primary reason for refrigerating urine specimens?
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What effect does refrigeration have on amorphous urate and phosphate crystals in urine?
What effect does refrigeration have on amorphous urate and phosphate crystals in urine?
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A urine specimen is preserved using commercial preservative tablets. What is a major consideration when using this method?
A urine specimen is preserved using commercial preservative tablets. What is a major consideration when using this method?
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What happens to red blood cell (RBC) and white blood cell (WBC) casts in an unpreserved urine sample that has been standing for an extended period?
What happens to red blood cell (RBC) and white blood cell (WBC) casts in an unpreserved urine sample that has been standing for an extended period?
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What is the primary reason for refrigerating urine specimens during the collection period?
What is the primary reason for refrigerating urine specimens during the collection period?
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In a pre- and post-massage test (PPMT), a positive result for significant bacteriuria is indicated when the post-massage specimen exhibits how many times the bacterial count of the pre-massage specimen?
In a pre- and post-massage test (PPMT), a positive result for significant bacteriuria is indicated when the post-massage specimen exhibits how many times the bacterial count of the pre-massage specimen?
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What error in urine collection is specifically associated with a timed collection?
What error in urine collection is specifically associated with a timed collection?
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In the Stamey-Meares test for prostatitis, which specimen represents the urethral specimen?
In the Stamey-Meares test for prostatitis, which specimen represents the urethral specimen?
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If a urine specimen requires a chemical preservative, what characteristic should the preservative possess?
If a urine specimen requires a chemical preservative, what characteristic should the preservative possess?
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Which of the following describes the correct method for collecting a catheterized urine specimen?
Which of the following describes the correct method for collecting a catheterized urine specimen?
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In the Stamey-Meares test, what is the correct order for collection?
In the Stamey-Meares test, what is the correct order for collection?
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What volume of urine is collected for VB1 and VB2 in the Stamey-Meares test?
What volume of urine is collected for VB1 and VB2 in the Stamey-Meares test?
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What is the primary reason for maintaining a strict chain of custody for urine specimens?
What is the primary reason for maintaining a strict chain of custody for urine specimens?
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Which type of identification is acceptable for the individual providing a urine specimen?
Which type of identification is acceptable for the individual providing a urine specimen?
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When is a 'witnessed' urine specimen collection typically indicated?
When is a 'witnessed' urine specimen collection typically indicated?
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If a witnessed urine specimen collection is required, who should observe the collection?
If a witnessed urine specimen collection is required, who should observe the collection?
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What volume of urine is typically collected during a witnessed urine specimen collection?
What volume of urine is typically collected during a witnessed urine specimen collection?
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After a witnessed or unwitnessed urine specimen collection, what is the immediate next step?
After a witnessed or unwitnessed urine specimen collection, what is the immediate next step?
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What is the main purpose of the 'chain of custody' documentation in drug testing?
What is the main purpose of the 'chain of custody' documentation in drug testing?
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What does the absence of a complete chain of custody documentation for a urine specimen imply?
What does the absence of a complete chain of custody documentation for a urine specimen imply?
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Flashcards
Urinalysis Introduction
Urinalysis Introduction
The study of urine specimens for diagnostic purposes.
Specimen Labeling
Specimen Labeling
Process of labeling urine specimens immediately after collection.
Types of Urine Specimens
Types of Urine Specimens
Different urine samples collected for specific testing needs.
Requisition Forms
Requisition Forms
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Specimen Handling
Specimen Handling
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Specimen Volume for Analysis
Specimen Volume for Analysis
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In vivo vs. In vitro
In vivo vs. In vitro
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Microbiological Studies
Microbiological Studies
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Time Sensitivity of Urine Specimens
Time Sensitivity of Urine Specimens
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Changes in Urine Color
Changes in Urine Color
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Decreased Clarity in Urine
Decreased Clarity in Urine
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Increased Ammonia Odor
Increased Ammonia Odor
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Increase in Urine pH
Increase in Urine pH
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Decreased Glucose in Urine
Decreased Glucose in Urine
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Decreased Bilirubin in Urine
Decreased Bilirubin in Urine
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Increased Nitrite in Urine
Increased Nitrite in Urine
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Specimen Preservation Method
Specimen Preservation Method
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Hydrochloric Acid
Hydrochloric Acid
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Acetic Acid
Acetic Acid
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Tartaric Acid
Tartaric Acid
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Formalin (Formaldehyde)
Formalin (Formaldehyde)
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Sodium Fluoride
Sodium Fluoride
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Random Specimen
Random Specimen
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First Morning Specimen
First Morning Specimen
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Midstream Clean-Catch Specimen
Midstream Clean-Catch Specimen
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Invalid Third Specimen Result
Invalid Third Specimen Result
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Specimen Refrigeration
Specimen Refrigeration
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Pre-and Post-Massage Test (PPMT)
Pre-and Post-Massage Test (PPMT)
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Significant Bacteriuria
Significant Bacteriuria
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Timed Collections Errors
Timed Collections Errors
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Stamey-Meares Test
Stamey-Meares Test
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Voided Bladder Samples
Voided Bladder Samples
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Expressed Prostatic Specimen (EPS)
Expressed Prostatic Specimen (EPS)
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Midstream Clean-Catch
Midstream Clean-Catch
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Bacterial Culture
Bacterial Culture
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Suprapubic Aspiration
Suprapubic Aspiration
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Cytologic Examination
Cytologic Examination
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Two-Hour Post Prandial
Two-Hour Post Prandial
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Glucose Tolerance Test
Glucose Tolerance Test
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Witnessed vs. Unwitnessed Collections
Witnessed vs. Unwitnessed Collections
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Identification Requirements
Identification Requirements
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Study Notes
Urine Specimen Analysis
- Urine analysis is a biohazardous substance, requiring Standard Precautions (gloves).
- Specimen collection containers must be clean, dry, leakproof, and ideally disposable.
- Screw-top lids are preferable to snap-on lids to prevent leaks.
- Wide-mouthed containers are recommended for ease of collection, especially from females.
- Containers should be clear for visual assessment of color and clarity.
- A recommended capacity of 50ml is sufficient for analysis and microscopic examination.
- Sterile containers are essential for microbiological studies.
- Specimens must be labeled with patient details, collection date and time, method of collection, and preservative(s) used.
Specimen Rejection Criteria
- Improperly labeled or unlabeled specimens.
- Mismatched labels and requisition forms.
- Specimens contaminated with feces or toilet paper.
- Contaminated container exteriors.
- Insufficient specimen volume.
- Improper transport or handling.
Specimen Handling and Preservation
- Deliver specimens to the laboratory promptly, ideally within 2 hours of collection.
- Refrigerate specimens if testing cannot be performed within 2 hours.
- Chemical preservatives can be used for longer-term storage (i.e., 24 hours).
- Refrigeration can induce precipitation of amorphous urates and phosphates.
- Preservatives must not interfere with analysis.
Types of Urine Specimens
- Random specimen: Collected at any time; convenient for routine screening but can be affected by recent food or activity.
- First morning specimen: Collected upon arising; highly concentrated; useful for detecting trace elements or for pregnancy tests.
- 24-hour specimen: Collected over a 24-hour period; used to measure substances with varying diurnal patterns.
- Catheterized specimen: Collected through a catheter; sterile; useful for bacterial culture and microbiological testing.
- Midstream clean-catch specimen: Collected in the middle of urination, after cleansing to minimize contamination.
- Suprapubic aspiration: Collected through a needle inserted into the bladder; sterile; often used in infants or when a specimen for bacterial culture is required.
- Three-glass collection: Collected using midstream clean-catch technique following prostate massage for detecting prostatitis.
- Four-glass collection: (Meares-Stamey test) A modified three-glass collection to detect prostatitis.
- Fasting (second morning) specimen: Collected after an overnight fast; used for monitoring glucose levels in diabetes.
- 2-hour post-prandial specimen: Collected 2 hours after eating, used for monitoring glucose levels in diabetes.
Additional Notes
- Urine temperature: The temperature of urine should be between 32.5°C and 37.7°C upon arrival in the laboratory; otherwise, repeat collection may be required.
- Urine Color: Important for detecting impurities or unusual substances in the urine.
- Urine pH: Urine pH greater than 9 may likely require a re-collection of the specimen.
- Chemical tests: Some chemical tests may be affected by improper specimen handling and storage.
- Preservation: Different methods of preservation exist and must be specified and applied accordingly.
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Description
This quiz covers essential guidelines for urine specimen collection and handling, highlighting the importance of cleanliness and appropriate container selection. It also outlines the criteria for specimen rejection, ensuring the integrity of the analysis process. Perfect for students and professionals in healthcare and laboratory settings.