Specimen Collection Techniques

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Questions and Answers

What is the primary purpose of a urine culture and sensitivity (C&S) test?

  • Measure glucose levels in urine
  • Check for the presence of blood in urine
  • Detect microorganisms and determine effective antibiotics (correct)
  • Analyze the color and clarity of urine

Which urine specimen collection method is recommended for a culture and sensitivity test?

  • First urine sample of the day
  • 24-hour urine collection
  • Clean catch mid-stream urine (correct)
  • Random urine sample

How should sputum specimens be collected for a C&S test?

  • Three morning specimens collected on consecutive days (correct)
  • One specimen in the afternoon after eating
  • Three specimens over three days, each in the evening
  • One specimen at any time after brushing teeth

What does the 24-hour urine collection primarily assess?

<p>Renal function and urine content (B)</p> Signup and view all the answers

When collecting a 24-hour urine sample, what is the first step that should be taken?

<p>Discard the first urine specimen collected at 7 am (C)</p> Signup and view all the answers

Which of the following tests is NOT indicated for urine specimens?

<p>Acid-fast bacillus test for tuberculosis (A)</p> Signup and view all the answers

What is a key principle when collecting a stool specimen for the occult blood test?

<p>Avoiding certain foods prior to collection is essential (A)</p> Signup and view all the answers

What should be avoided before collecting sputum specimens for AFB testing?

<p>Brushing teeth with toothpaste (C)</p> Signup and view all the answers

What is indicated by a glucosetronic test on blood?

<p>Blood glucose levels (D)</p> Signup and view all the answers

Flashcards

FEME (Full Examination Microscopic Examination)

A type of urine test that involves examining the urine under a microscope to identify any abnormalities.

C & S (Culture & Sensitivity)

A type of urine test that involves culturing urine samples to identify the type of bacteria present and then testing its sensitivity to different antibiotics.

Dipstick Urine Test

A urine test that measures the amount of glucose in the urine.

24-Hour Urine Collection

A type of urine test that involves collecting all the urine produced over a 24-hour period to assess kidney function and urine composition.

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Occult Blood Test

A stool test that measures the presence of blood in the stool.

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Ova & Cyst Test

A stool test that examines the stool for parasites like eggs and cysts.

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Sputum C & S (Culture & Sensitivity)

A sputum test that involves culturing sputum samples to identify the type of bacteria present and then testing its sensitivity to different antibiotics.

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AFB (Acid-Fast Bacillus) Test

A sputum test performed to detect the presence of Mycobacterium tuberculosis, the bacteria responsible for Tuberculosis.

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Dextrostic Blood Test

A blood test used to measure the level of glucose (sugar) in the blood.

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Swab C & S (Culture & Sensitivity)

A test that involves collecting a swab sample from a mucous membrane or discharge (e.g., throat, nose, wound) to culture the bacteria and determine its sensitivity to antibiotics.

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Study Notes

Specimen Collection

  • Specimen collection is crucial for investigations
  • Different specimens include urine, stool, sputum, blood, and swabs.

Urine Specimen Collection

  • FEME (Full Examination): Involves a full microscopic examination
  • C&S (Culture & Sensitivity): Identifies microorganisms and determines antibiotic susceptibility
  • Urinalysis dipstick: A quick test for various substances in urine
  • 24-hour urine collection: Collects urine over a 24-hour period
  • Urinalysis: Measurements of urine pH, specific gravity (SG), protein, glucose, blood, and other substances

Stool Specimen Collection

  • Collection methods include using a specimen pot and spoon
  • Faeces should be semi-formed or liquid
  • Fill specimen pot to approximately a quarter full
  • Hand hygiene is essential after collection

Sputum Specimen Collection

  • C&S: One sputum specimen collected
  • AFB-PTB: Collecting 3 specimens over 3 days
  • Early morning collection is recommended
  • Sputum should be collected before brushing teeth, taking food, use of tooth paste or antiseptic
  • Sputa collection uses a sputum trap and a suction catheter
  • Must be different from saliva, characterized by a thick and sticky nature

Blood Specimen Collection

  • Blood tests: Glucose level is a common test using a glucometer or dextrostic device.
  • Swab test (C&S): Assess mucous membranes for infections (throat, nasal, wound discharge)
  • To determine levels of glucose in the blood

Swab Collection

  • Sterile swabs are crucial for collecting samples
  • Throat swabs involve swabbing the tonsillar areas and posterior pharynx, targeting inflamed areas

Urinometer

  • Measures the specific gravity of urine to analyze the concentration of solutes
  • Reference ranges for urine specific gravity are 1.005-1.030
  • Elevated SG may indicate dehydration, diarrhea, vomiting, excessive sweating, kidney/bladder infections, or diabetes.

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