Urine Sediment Examination Quiz

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Questions and Answers

Which type of cell is primarily associated with renal transplant rejection?

  • Lymphocytes (correct)
  • Monocytes
  • Epithelial Cells
  • Neutrophils

What characteristic is used to differentiate Candida albicans from other yeasts in urine sediment?

  • Budding structures (correct)
  • Formation of chains
  • Presence of hyphae
  • Large cell size

Which type of epithelial cells are larger than red blood cells and clinically significant due to the presence of lipids?

  • Squamous Epithelial Cells
  • Renal Tubular Epithelial Cells (correct)
  • Glitter Cells
  • Transitional Epithelial Cells

Which bacteria are commonly noted for their motility in urine sediment examination?

<p>Enterobacteriaceae (D)</p> Signup and view all the answers

What effect does the Sternheimer-Malbin stain have on urine sediment constituents?

<p>Enhances visualization of cellular details (D)</p> Signup and view all the answers

Which characteristic is associated with Trichomonas vaginalis?

<p>Pear-shaped with jerky motility (B)</p> Signup and view all the answers

What is an indicator of bladder cancer found in urine analysis?

<p>BTA (Bladder Tumor Antigen) (C)</p> Signup and view all the answers

How many epithelial cells would classify as 'few' in a urine sample according to the quantification table?

<p>0-5 (A)</p> Signup and view all the answers

What is the minimum number of mucus threads that characterizes the 'rare' category in the quantification table?

<p>0 (C)</p> Signup and view all the answers

What type of casts are noted as the starting point for all types of casts?

<p>Hyaline casts (A)</p> Signup and view all the answers

What is the minimum volume of urine required for microscopic examination without centrifugation?

<p>1 ml (B)</p> Signup and view all the answers

Which objective lenses are recommended for further identification of abnormalities during microscopic examination?

<p>10x LPO and 10x HPO (B)</p> Signup and view all the answers

Which of the following substances can lead to false positives when conducting a reagent strip reaction for leukocytes?

<p>Nitrofurantoin (A)</p> Signup and view all the answers

What is the primary advantage of using a parfocal microscope?

<p>Minimal adjustments are needed when changing objectives. (D)</p> Signup and view all the answers

What is the best method for cleaning contaminated optical surfaces of a microscope?

<p>Commercial lens cleaner (C)</p> Signup and view all the answers

Which of the following correctly describes ascorbic acid in relation to urine analysis?

<p>It interferes with some reagent strips. (B)</p> Signup and view all the answers

What is the recommended time duration for the reagent strip reaction for leukocytes?

<p>120 seconds (B)</p> Signup and view all the answers

What is the normal upper limit for hyaline casts in a 12-hour urine sample?

<p>5,000/12-hr urine (C)</p> Signup and view all the answers

What is the primary function of the coarse adjustment knob on a binocular microscope?

<p>To perform initial focusing when using low power objectives (D)</p> Signup and view all the answers

Which part of the microscope is responsible for adjusting the size of the light beam reaching the slide?

<p>Field Diaphragm Control Ring (D)</p> Signup and view all the answers

What microscopy technique is best suited for enhancing visualization of translucent elements in specimens?

<p>Phase-contrast Microscopy (C)</p> Signup and view all the answers

Which stain or dye is used primarily to differentiate between Gram-positive and Gram-negative bacteria?

<p>Gram Stain (D)</p> Signup and view all the answers

Which microscopy technique requires the insertion of two polarizing filters?

<p>Polarizing Microscopy (C)</p> Signup and view all the answers

What is the role of the rheostat in a microscope?

<p>Regulates the intensity of the light (D)</p> Signup and view all the answers

Which component is utilized to enhance clarity when using high power or oil immersion objectives?

<p>Fine Adjustment Knob (B)</p> Signup and view all the answers

Which stain is useful for identifying urinary eosinophils?

<p>Hansel Stain (B)</p> Signup and view all the answers

What does the condenser do in a microscope?

<p>Focuses and controls light on the specimen (B)</p> Signup and view all the answers

Which of the following microscopy techniques is primarily used to visualize fluorescent substances?

<p>Fluorescence Microscopy (D)</p> Signup and view all the answers

What is the appearance of amorphous urates in urine?

<p>Fluffy orange or pink sediment (D)</p> Signup and view all the answers

Which type of crystal is described as having a 'cigarette-butt appearance'?

<p>Calcium Sulfate (A)</p> Signup and view all the answers

What happens to ammonium biurate in the presence of acetic acid?

<p>It turns into uric acid (D)</p> Signup and view all the answers

What is a common characteristic of triple phosphate crystals?

<p>Colorless elongated rectangular or rhomboid plates (A)</p> Signup and view all the answers

Which type of crystal is characterized by the presence of urea-splitting bacteria in urine?

<p>Ammonium Biurate (C)</p> Signup and view all the answers

Which crystal form is considered rare and resembles 'thorny apples'?

<p>Ammonium Biurate (A)</p> Signup and view all the answers

How do amorphous phosphates typically appear in alkaline urine?

<p>Granular or lacy white precipitate (C)</p> Signup and view all the answers

What is the likely shape of monosodium urates found in urine?

<p>Small, slender colorless needles (B)</p> Signup and view all the answers

What is the primary significance of RBC casts in urine analysis?

<p>Indicates bleeding within the nephron (B)</p> Signup and view all the answers

Which type of cast is suggested by the presence of inflammation or infection in the nephron?

<p>WBC cast (A)</p> Signup and view all the answers

What distinguishes a pseudoleukocyte cast from other casts?

<p>It is noted in lower urinary tract infections but not reported as a cast (D)</p> Signup and view all the answers

Which type of cast is characterized by a 'sandpaper appearance' and indicates diverse renal conditions?

<p>Granular cast (B)</p> Signup and view all the answers

What type of cast indicates extreme urine stasis and renal failure?

<p>Broad cast (A)</p> Signup and view all the answers

Which of the following casts contains fat globules not stained by Sternheimer-Malbin stain?

<p>Fatty cast (B)</p> Signup and view all the answers

What is the identifying feature of a waxy cast?

<p>Highly refractile with jagged ends (A)</p> Signup and view all the answers

What causes the formation of crystals in urine?

<p>Precipitation of urine solutes (D)</p> Signup and view all the answers

Which cast is best confirmed through a Gram stain?

<p>Bacterial cast (B)</p> Signup and view all the answers

What does the presence of abnormal crystals in urine often suggest?

<p>Concentrated urine specimens (C)</p> Signup and view all the answers

What is the color and typical morphology of cystine crystals?

<p>Colorless, often laminated hexagonal plates (A)</p> Signup and view all the answers

Which crystal type is associated with liver disease and commonly presents in rosettes?

<p>Tyrosine (D)</p> Signup and view all the answers

What are the identifying characteristics of leuine crystals?

<p>Yellow-brown ovoid spheres with concentric circles (A)</p> Signup and view all the answers

Which of the following crystal types is not typically associated with liver disease?

<p>Uric Acid (B)</p> Signup and view all the answers

What is a distinguishing feature of sulfonamide crystals?

<p>Fan-shaped or arrowhead shaped crystals (B)</p> Signup and view all the answers

Indinavir sulfate crystals are best characterized by which of the following features?

<p>Colorless needles or rectangular plates (C)</p> Signup and view all the answers

Which of the following crystals can often be mistaken for calcium phosphate due to their morphology?

<p>Sulfonamides (A)</p> Signup and view all the answers

What is a unique characteristic of hemosiderin crystals under a microscope?

<p>Coarse, yellow-brown granules (B)</p> Signup and view all the answers

Which abnormal acid crystal has a characteristic appearance of colorless, refractile hexagonal plates?

<p>Cystine (D)</p> Signup and view all the answers

What is the solubility characteristic of cholesterol crystals in urine?

<p>Soluble in chloroform (A)</p> Signup and view all the answers

Which type of crystal is associated with liver disease and appears as fine colorless to yellow needles forming clumps or rosettes?

<p>Tyrosine (A)</p> Signup and view all the answers

Indinavir Sulfate crystals are associated with which clinical condition?

<p>Renal blockage in HIV-positive individuals (A)</p> Signup and view all the answers

What gives leuicine crystals their unique appearance?

<p>Oily spheres with concentric circles (C)</p> Signup and view all the answers

Which crystal type is not soluble in water and requires specific solvents like acetic acid for solubility?

<p>Bilirubin (C)</p> Signup and view all the answers

Which abnormal crystal type is specifically noted for forming a Maltese Cross under polarization microscopy?

<p>Cholesterol (C)</p> Signup and view all the answers

What condition is most commonly linked to the presence of uric acid crystals in urine?

<p>Cystinuria (C)</p> Signup and view all the answers

What shape do sulfamethoxazole crystals typically exhibit?

<p>Brown rosettes or spheres (B)</p> Signup and view all the answers

Which abnormal crystal type can lead to observations resembling artifacts, causing potential errors in urine sample analysis?

<p>Uric Acid (C)</p> Signup and view all the answers

Flashcards

Urine Microscopic Examination

A microscopic examination technique that focuses on analyzing solid particles (sediment) in urine. This helps to identify various cells, casts, and other components that can indicate underlying health conditions.

Urine Sediment Preparation

The process of separating urine into layers using a centrifuge, allowing the sediment to be collected for microscopic analysis.

Addis Count

A method of accurately quantifying specific components in a 12-hour urine sample, such as red blood cells, white blood cells, and casts.

Reagent Strip Test for Leukocytes

A reagent strip test that measures the activity of leukocyte esterase, an enzyme present in white blood cells.

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Ascorbic Acid (Vitamin C)

A water-soluble vitamin that can interfere with certain urine reagent strips, potentially causing inaccurate results.

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Objective Lens

The part of a microscope that focuses on the specimen, allowing for detailed examination of the sediment.

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Resolution

The ability of a microscope to distinguish between two closely spaced objects.

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Dust Removal from Microscope

A technique for removing dust from the optical surfaces of a microscope using a specialized brush.

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What are microscope objectives?

The primary lenses that magnify the specimen and form the initial image.

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What does the coarse adjustment knob do?

A knob used for initial focusing, especially with lower-power objectives. It moves the stage up and down noticeably, bringing the specimen into view.

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What does the fine adjustment knob do?

A knob used for fine adjustments, especially for higher-power objectives. It makes subtle movements to sharpen the image.

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What is phase-contrast microscopy?

A technique that enhances visualization of translucent elements by converting bright-field light into phase contrast. Requires a special objective and condenser.

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What is polarizing microscopy?

A technique used to identify birefringent substances, often revealing the presence of cholesterol or specific crystals. Requires two polarizing filters.

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What is fluorescence microscopy?

A technique used to visualize fluorescent substances or specimens. It requires a special condenser that blocks incident light and allows only exciting light to reach the sample.

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What is the Gram stain used for?

A stain used to differentiate Gram-positive and Gram-negative bacteria based on their cell wall structure.

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What is the Toluidine blue stain used for?

A stain commonly used in urine microscopy to highlight the nuclei of cells. It also lyses red blood cells, making it easier to distinguish white blood cells.

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What is 2% acetic acid used for in urine analysis?

A stain often used in urine microscopy to differentiate red blood cells from white blood cells, yeast, oil droplets and crystals.

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What is the Hansel stain used for?

A stain used for identifying urinary eosinophils.

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Hematuria

Red blood cells in the urine, indicating blood in the urinary tract.

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Leukocyturia (Pyuria)

White blood cells in the urine, suggesting infection or inflammation.

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Transitional Epithelial Cells (TECs)

Derived from the renal pelvis, calyces, ureter, bladder, and upper male urethra. Can vary in shape and size. Abnormal morphology can indicate malignancy or viral infection.

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Renal Tubular Epithelial Cells (RTEs)

Larger than RBCs, often clinically significant. May contain lipids and have diverse morphologies.

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Squamous Epithelial Cells (SECs)

Largest epithelial cells, derived from vagina, female urethra, and lower male urethra. May be folded.

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What is Trichomonas vaginalis?

A pear-shaped, single-celled parasite with a characteristic undulating membrane, often seen moving in a jerky fashion in urine specimens. This organism is linked to sexually transmitted infections.

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What is Schistosoma haematobium?

A type of blood fluke, often found in urine specimens as eggs with a terminal spine. These eggs can cause hematuria (blood in the urine) and are associated with bladder cancer.

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What are casts in urine?

These are cylindrical structures, often found in urine sediment, that form in the renal tubules of the kidneys. They are composed of protein and can contain various cellular elements, providing valuable clues to kidney health.

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What is NMP22?

A protein marker used to detect the presence of bladder cancer. Elevated levels of this marker can indicate a potential malignancy.

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What are Hyaline casts?

Hyaline casts, are the simplest type of urinary cast, composed primarily of protein. They can be seen in people with different levels of kidney health. However, they are considered the foundation for the formation of all other types of casts.

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Amorphous urates

Fluffy orange or pink sediment found in urine. Often resembles brick dust.

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Uric acid

A major product of purine metabolism, appearing in various shapes like diamonds, plates, or football-like structures.

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Weddelite

A type of calcium oxalate crystal found in urine, recognized by its distinctive emerald-cut diamond shape.

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Whewellite

A type of calcium oxalate crystal found in urine; its shape is not as easily recognizable as Weddelite.

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Monosodium urates

Rare, smaller needle-like or spherical clusters of urates found in urine. Often associated with gout.

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Amorphous phosphates

The most common cause of urine turbidity in alkaline conditions. It appears as a fine, white precipitate.

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Ammonium biurate

A characteristic 'thorny apple' shaped crystal often seen in alkaline urine.

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Triple phosphate

A clear, rectangular-shaped crystal common in alkaline urine. Also known as magnesium ammonium phosphate.

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RBC Cast

Most fragile cast, signifying bleeding within the nephron. Easily identified by its orange-red color, often associated with glomerulonephritis or strenuous exercise.

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WBC Cast

Indicates inflammation or infection within the nephron, resembling RTE cast. Differentiation requires phase microscopy and supravital stain.

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Pseudoleukocyte Cast

A clump of leukocytes, commonly observed in lower urinary tract infections, not considered a true cast.

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Epithelial Cast

Indicates advanced tubular damage or destruction, featuring smaller, round, and oval cells visible on the cast matrix.

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Bacterial Cast

Presence confirms bacterial infection within the nephron, typically associated with pyelonephritis. Requires Gram stain for confirmation.

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Granular Cast

Derived from lysosomes of renal tubular epithelial cells during normal metabolism. Cells disintegrate when the cast is retained in the tubule. Coarse casts contain larger granules.

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Fatty Cast

Contains fat globules, not stained by Sternheimer-Malbin stain. Requires lipid stains or a polarizing microscope for identification.

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Waxy Cast

The final degenerative form of various casts, brittle, highly refractile, with jagged ends. Indicates chronic renal failure or end-stage renal disease.

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Broad Cast

Indicates destruction or widening of the tubular walls, often referred to as a 'Renal Failure' cast.

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Crystals in Urine

Crystals formed by precipitation of urine solutes, often seen in concentrated urine specimens. Abnormal crystals can indicate specific conditions.

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Cystine Crystals

Colorless, often laminated hexagonal plates that can be mistaken for hexagonal uric acid crystals. These crystals are associated with cystinuria, a rare metabolic disorder where the body cannot properly reabsorb cystine.

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Uric Acid Crystals

Yellow-brown crystals with various shapes, including rhombic, barrel-shaped, and rosettes. Associated with high levels of uric acid in the urine, which can be a sign of gout, kidney stones, or other conditions.

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Cholesterol Crystals

Colorless, refractile hexagonal plates that resemble flat, shiny shards of glass. Often associated with high cholesterol levels, possibly indicating a medical condition.

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Tyrosine Crystals

Fine, colorless to yellow needles that often appear in clumps or rosettes. Frequently associated with liver disease and other conditions.

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Leucine Crystals

Yellow-brown, ovoid spheres with concentric circles and radial striations (lines radiating from the center). Frequently associated with liver disease.

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Bilirubin Crystals

Clumped granules or needles, varying in color (yellowish-green to brown). Associated with liver disease and elevated bilirubin levels in the urine.

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Sulfonamide Crystals

Fan-shaped, wheat-shaped, or arrowhead-shaped crystals that can be mistaken for calcium phosphate. Their solubility properties can help distinguish them. Associated with medication use, specifically sulfonamide antibiotics.

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Ampicillin Crystals

Colorless needles that may form clusters or bundles. Associated with the use of ampicillin, a penicillin antibiotic.

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What are cystine crystals?

Colorless, highly refractile hexagonal plates often with lamination, sometimes hexagonal sides. They are soluble in alkali and heat.

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What are uric acid crystals?

Yellow-brown, often seen as rosettes or dumbbells. Insoluble in acetic acid but soluble in alkali. Their presence can signify elevated uric acid levels.

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What are cholesterol crystals?

Colorless, flat, rectangular plates with notched corners. They are soluble in chloroform but insoluble in water.

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What are tyrosine crystals?

Colorless to yellow needles, often arranged in clumps or rosettes. They readily dissolve in alkali.

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What are leucine crystals?

Yellow-brown, oily spheres with concentric circles. They are soluble in hot alkali and alcohol.

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What are bilirubin crystals?

Bright yellow, clumped granules or needles, or reddish-brown needles clustered in spheres. They are soluble in acetic acid, hydrochloric acid, sodium hydroxide, acetone, and chloroform.

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What are sulfamethoxazole crystals?

Brown rosettes or spheres with irregular radial striations.

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What are acetylsulfadiazine crystals?

Yellow-brown sheaves of wheat with eccentric binding, associated with the use of specific antibiotics.

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What are sulfadiazine crystals?

Yellow-brown sheaves of wheat with eccentric binding.

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What are ampicillin crystals?

Colorless needles that tend to form bundles when refrigerated.

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