Podcast
Questions and Answers
What was the key gene that the biochemist used as a reporter for pluripotency?
What was the key gene that the biochemist used as a reporter for pluripotency?
- CDK2
- BRCA1
- Fbx15 (correct)
- PTEN
Why did the biochemist choose to start with fibroblasts in his experiment?
Why did the biochemist choose to start with fibroblasts in his experiment?
- They are more resistant to pluripotency transformation.
- They are easy to obtain from the skin. (correct)
- They lack the Fbx15 gene.
- They are already pluripotent cells.
What was the main challenge the biochemist faced in his experiment with fibroblasts?
What was the main challenge the biochemist faced in his experiment with fibroblasts?
- Activating the Fbx15 gene in pluripotent cells.
- Ensuring proper cell differentiation.
- Identifying the less important factors among the 24.
- Inducing pluripotency in non-pluripotent cells. (correct)
What did the biochemist need to establish as step number one of his experiment?
What did the biochemist need to establish as step number one of his experiment?
Why was the Fbx15 gene crucial in the biochemist's experiment?
Why was the Fbx15 gene crucial in the biochemist's experiment?
What did the biochemist use the Fbx15 gene as in his experiment?
What did the biochemist use the Fbx15 gene as in his experiment?
What was the biochemist trying to achieve by switching on the Fbx15 gene in fibroblasts?
What was the biochemist trying to achieve by switching on the Fbx15 gene in fibroblasts?
Why did the biochemist consider identifying the most important factors among the 24 crucial?
Why did the biochemist consider identifying the most important factors among the 24 crucial?
What strategy did the biochemist employ to potentially induce pluripotency in fibroblasts?
What strategy did the biochemist employ to potentially induce pluripotency in fibroblasts?
What role did John Gurdon play in the biochemist's experimental design?
What role did John Gurdon play in the biochemist's experimental design?
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