Cell Differentiation and Neuronal Generation
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Questions and Answers

What was the key gene that the biochemist used as a reporter for pluripotency?

  • CDK2
  • BRCA1
  • Fbx15 (correct)
  • PTEN
  • Why did the biochemist choose to start with fibroblasts in his experiment?

  • They are more resistant to pluripotency transformation.
  • They are easy to obtain from the skin. (correct)
  • They lack the Fbx15 gene.
  • They are already pluripotent cells.
  • What was the main challenge the biochemist faced in his experiment with fibroblasts?

  • Activating the Fbx15 gene in pluripotent cells.
  • Ensuring proper cell differentiation.
  • Identifying the less important factors among the 24.
  • Inducing pluripotency in non-pluripotent cells. (correct)
  • What did the biochemist need to establish as step number one of his experiment?

    <p>Developing an assay to recognize pluripotency.</p> Signup and view all the answers

    Why was the Fbx15 gene crucial in the biochemist's experiment?

    <p>It was always active in pluripotent cells.</p> Signup and view all the answers

    What did the biochemist use the Fbx15 gene as in his experiment?

    <p>A reporter for pluripotency.</p> Signup and view all the answers

    What was the biochemist trying to achieve by switching on the Fbx15 gene in fibroblasts?

    <p>Making the fibroblasts pluripotent.</p> Signup and view all the answers

    Why did the biochemist consider identifying the most important factors among the 24 crucial?

    <p>To understand pluripotency better.</p> Signup and view all the answers

    What strategy did the biochemist employ to potentially induce pluripotency in fibroblasts?

    <p>Activating the Fbx15 gene.</p> Signup and view all the answers

    What role did John Gurdon play in the biochemist's experimental design?

    <p>He inspired using fibroblasts as a starting point for cell conversion.</p> Signup and view all the answers

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