20 Questions
Which technique is currently used by plant scientists for targeted genome editing?
CRISPR-Cas system
What does CRISPR stand for?
Clustered Regularly Interspaced Short Palindromic Repeats
What is the function of CRISPRs in bacteria?
To record previous viral infections
What do Cas genes encode for in bacteria?
Adaptive immune system
What is the main difference between genome editing and genetic engineering (GE)?
GE refers to a broader category of techniques while genome editing is a specific technique
What is the purpose of the CRISPR-Cas system in bacteria?
To detect and fight subsequent viral attacks
Which component of the CRISPR-Cas system is needed for genome editing?
Cas9 enzyme
What is the role of the guide RNA in the CRISPR-Cas9 process?
To locate and bind to the targeted DNA sequence
What happens when the Cas9 enzyme cuts the DNA at the targeted location?
The plant cell's repair machinery re-attaches the cut DNA ends
Which of the following is NOT a potential improvement that can be achieved through genome editing in crops?
Reduced water availability
What is the purpose of including a repair template during the plant transformation process?
To direct the repair of the genomic DNA at the cut site
Which crop was the first to have a commercially available genome-edited food product in 2019?
Soybean
Which of the following is NOT mentioned as a potential CRISPR-Cas delivery method?
Viral-mediated delivery
What is the purpose of using plasmid-mediated delivery in genome editing?
To carry the genes for the guide RNA and Cas that destroys the crop protein
Which factor influences the choice of CRISPR-Cas delivery method?
The type of plant being edited
What is the goal of direct delivery of the Cas9 protein with guide RNA into plant cells?
To provide a solution to the citrus greening disease
What is the purpose of improving the cacao plant's ability to fight a virus in West Africa?
To protect the cacao crop in West Africa from a virus
Which one of these is NOT mentioned as a factor influencing the choice of CRISPR-Cas delivery method?
The availability of funding
What is the purpose of using direct delivery of the Cas9 protein with guide RNA into plant cells?
To provide a solution to the citrus greening disease
Which one of these is mentioned as a potential CRISPR-Cas delivery method?
Plasmid-mediated delivery
Test your knowledge on targeted genome editing in food agriculture with this quiz. Learn about the CRISPR-Cas system and other techniques used by plant scientists to precisely edit genes in the genome.
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