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Questions and Answers

Which technique is currently used by plant scientists for targeted genome editing?

  • rDNA techniques
  • Previous GE methods
  • CRISPR-Cas system (correct)
  • Random integration

What does CRISPR stand for?

  • Complementary RNA Identification System
  • Clustered Regularly Interspaced Short Palindromic Repeats (correct)
  • Cas Protein Encoding System
  • Cutting and Modifying DNA

What is the function of CRISPRs in bacteria?

  • To produce small non-coding RNAs
  • To record previous viral infections (correct)
  • To incorporate viral DNA into the genome
  • To identify and fight bacterial infections

What do Cas genes encode for in bacteria?

<p>Adaptive immune system (A)</p> Signup and view all the answers

What is the main difference between genome editing and genetic engineering (GE)?

<p>GE refers to a broader category of techniques while genome editing is a specific technique (A)</p> Signup and view all the answers

What is the purpose of the CRISPR-Cas system in bacteria?

<p>To detect and fight subsequent viral attacks (C)</p> Signup and view all the answers

Which component of the CRISPR-Cas system is needed for genome editing?

<p>Cas9 enzyme (B)</p> Signup and view all the answers

What is the role of the guide RNA in the CRISPR-Cas9 process?

<p>To locate and bind to the targeted DNA sequence (D)</p> Signup and view all the answers

What happens when the Cas9 enzyme cuts the DNA at the targeted location?

<p>The plant cell's repair machinery re-attaches the cut DNA ends (D)</p> Signup and view all the answers

Which of the following is NOT a potential improvement that can be achieved through genome editing in crops?

<p>Reduced water availability (B)</p> Signup and view all the answers

What is the purpose of including a repair template during the plant transformation process?

<p>To direct the repair of the genomic DNA at the cut site (C)</p> Signup and view all the answers

Which crop was the first to have a commercially available genome-edited food product in 2019?

<p>Soybean (C)</p> Signup and view all the answers

Which of the following is NOT mentioned as a potential CRISPR-Cas delivery method?

<p>Viral-mediated delivery (D)</p> Signup and view all the answers

What is the purpose of using plasmid-mediated delivery in genome editing?

<p>To carry the genes for the guide RNA and Cas that destroys the crop protein (B)</p> Signup and view all the answers

Which factor influences the choice of CRISPR-Cas delivery method?

<p>The type of plant being edited (A)</p> Signup and view all the answers

What is the goal of direct delivery of the Cas9 protein with guide RNA into plant cells?

<p>To provide a solution to the citrus greening disease (D)</p> Signup and view all the answers

What is the purpose of improving the cacao plant's ability to fight a virus in West Africa?

<p>To protect the cacao crop in West Africa from a virus (D)</p> Signup and view all the answers

Which one of these is NOT mentioned as a factor influencing the choice of CRISPR-Cas delivery method?

<p>The availability of funding (B)</p> Signup and view all the answers

What is the purpose of using direct delivery of the Cas9 protein with guide RNA into plant cells?

<p>To provide a solution to the citrus greening disease (D)</p> Signup and view all the answers

Which one of these is mentioned as a potential CRISPR-Cas delivery method?

<p>Plasmid-mediated delivery (A)</p> Signup and view all the answers

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