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Questions and Answers

Which technique is currently used by plant scientists for targeted genome editing?

  • rDNA techniques
  • Previous GE methods
  • CRISPR-Cas system (correct)
  • Random integration

    • What does CRISPR stand for?

  • Complementary RNA Identification System
  • Clustered Regularly Interspaced Short Palindromic Repeats (correct)
  • Cas Protein Encoding System
  • Cutting and Modifying DNA

    • What is the function of CRISPRs in bacteria?

  • To produce small non-coding RNAs
  • To record previous viral infections (correct)
  • To incorporate viral DNA into the genome
  • To identify and fight bacterial infections

    • What do Cas genes encode for in bacteria?

    <p>Adaptive immune system</p> Signup and view all the answers

    What is the main difference between genome editing and genetic engineering (GE)?

    <p>GE refers to a broader category of techniques while genome editing is a specific technique</p> Signup and view all the answers

    What is the purpose of the CRISPR-Cas system in bacteria?

    <p>To detect and fight subsequent viral attacks</p> Signup and view all the answers

    Which component of the CRISPR-Cas system is needed for genome editing?

    <p>Cas9 enzyme</p> Signup and view all the answers

    What is the role of the guide RNA in the CRISPR-Cas9 process?

    <p>To locate and bind to the targeted DNA sequence</p> Signup and view all the answers

    What happens when the Cas9 enzyme cuts the DNA at the targeted location?

    <p>The plant cell's repair machinery re-attaches the cut DNA ends</p> Signup and view all the answers

    Which of the following is NOT a potential improvement that can be achieved through genome editing in crops?

    <p>Reduced water availability</p> Signup and view all the answers

    What is the purpose of including a repair template during the plant transformation process?

    <p>To direct the repair of the genomic DNA at the cut site</p> Signup and view all the answers

    Which crop was the first to have a commercially available genome-edited food product in 2019?

    <p>Soybean</p> Signup and view all the answers

    Which of the following is NOT mentioned as a potential CRISPR-Cas delivery method?

    <p>Viral-mediated delivery</p> Signup and view all the answers

    What is the purpose of using plasmid-mediated delivery in genome editing?

    <p>To carry the genes for the guide RNA and Cas that destroys the crop protein</p> Signup and view all the answers

    Which factor influences the choice of CRISPR-Cas delivery method?

    <p>The type of plant being edited</p> Signup and view all the answers

    What is the goal of direct delivery of the Cas9 protein with guide RNA into plant cells?

    <p>To provide a solution to the citrus greening disease</p> Signup and view all the answers

    What is the purpose of improving the cacao plant's ability to fight a virus in West Africa?

    <p>To protect the cacao crop in West Africa from a virus</p> Signup and view all the answers

    Which one of these is NOT mentioned as a factor influencing the choice of CRISPR-Cas delivery method?

    <p>The availability of funding</p> Signup and view all the answers

    What is the purpose of using direct delivery of the Cas9 protein with guide RNA into plant cells?

    <p>To provide a solution to the citrus greening disease</p> Signup and view all the answers

    Which one of these is mentioned as a potential CRISPR-Cas delivery method?

    <p>Plasmid-mediated delivery</p> Signup and view all the answers

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