Understanding Retroviruses and Reverse Transcription
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Questions and Answers

What is the diameter of the spherical enveloped particle of retroviruses?

  • ~80-100nm (correct)
  • ~10-20nm
  • ~200-250nm
  • ~50-60nm

Which proteins are derived from the pol gene in retroviruses?

  • MA (matrix), CA (capsid), NC (nucleocapsid)
  • SU (external surface protein, also called gp120) and TM (transmembrane protein, also called gp24)
  • MA (matrix), CA (capsid), NC (nucleocapsid)
  • PR (protease), RT (reverse transcriptase), IN (integrase) (correct)

What is the function of the gag gene in retroviruses?

  • Encoding of envelope proteins: SU (external surface protein, also called gp120) and TM (transmembrane protein, also called gp24)
  • Involvement in genome replication and integration
  • Cleavage of polyprotein into mature proteins
  • Production of group-specific antigen proteins: MA (matrix), CA (capsid), NC (nucleocapsid) (correct)

What is the initial step in the unique replication cycle of retroviruses?

<p>Reverse transcription to produce a DNA copy of the RNA genome (A)</p> Signup and view all the answers

What is the role of reverse transcriptase in retroviruses?

<p>To produce a DNA copy of the RNA genome (B)</p> Signup and view all the answers

Where does the DNA enter after being made from the ssRNA genome by viral enzyme integrase?

<p>Nucleus with the viral enzyme integrase (D)</p> Signup and view all the answers

What is bound to genomic RNA by base pairing with primer binding sequences downstream of U5?

<p>A specific cellular tRNA (D)</p> Signup and view all the answers

What are the two subfamilies into which retroviruses were reclassified?

<p>Orthoretrovirinae and Spumaretrovirinae subfamilies (B)</p> Signup and view all the answers

What is the function of Ppt in retroviruses?

<p>It is used during reverse transcription (D)</p> Signup and view all the answers

What is the main basis for the tropism of retroviruses?

<p>Interaction of SU with cellular receptor (D)</p> Signup and view all the answers

What is the role of ribonuclease H (RNAse H) in reverse transcription?

<p>Specifically degrades the RNA part of the RNA:DNA hybrid (D)</p> Signup and view all the answers

What facilitates the DNA polymerase activity during reverse transcription?

<p>Virus-associated cellular tRNA (B)</p> Signup and view all the answers

Which type of genome does HIV possess?

<p>+ssRNA (positive sense single-stranded RNA) (C)</p> Signup and view all the answers

What causes depletion of CD4+ T cells in HIV-infected individuals?

<p>All of the above (D)</p> Signup and view all the answers

What is the main basis for the tropism of retroviruses?

<p>Interaction of SU with cellular receptor (A)</p> Signup and view all the answers

What is the diameter of the spherical enveloped particle of retroviruses?

<p>~100nm (C)</p> Signup and view all the answers

What facilitates the DNA polymerase activity during reverse transcription?

<p>Virus-associated cellular tRNA (D)</p> Signup and view all the answers

What is the role of ribonuclease H (RNAse H) in reverse transcription?

<p>Specifically degrades the RNA part of the RNA:DNA hybrid (D)</p> Signup and view all the answers

What is bound to genomic RNA by base pairing with primer binding sequences downstream of U5?

<p>Virus-associated cellular tRNA (B)</p> Signup and view all the answers

Which proteins are derived from the pol gene in retroviruses?

<p>Reverse transcriptase and integrase (A)</p> Signup and view all the answers

What causes depletion of CD4+ T cells in HIV-infected individuals?

<p>HIV replicating in and killing lymphocytes and macrophages (D)</p> Signup and view all the answers

What is the function of Ppt in retroviruses?

<p>Ppt is polypurine tract used during reverse transcription (C)</p> Signup and view all the answers

What is the function of the env gene in retroviruses?

<p>Codes for external surface protein and transmembrane protein (C)</p> Signup and view all the answers

What is the role of the U3 region in retroviruses?

<p>It is a splice site (B)</p> Signup and view all the answers

What is the function of the protease (PR) protein in retroviruses?

<p>Cleaves the polyprotein into mature proteins (B)</p> Signup and view all the answers

What is the specific cellular component bound to genomic RNA by base pairing with primer binding sequences (PBS) downstream of U5?

<p>Transfer RNA (tRNA) (B)</p> Signup and view all the answers

What is the primary function of reverse transcriptase (RT) in retroviruses?

<p>Catalyzes the synthesis of DNA from RNA template (D)</p> Signup and view all the answers

What is the role of nucleocapsid protein in retroviruses?

<p>Packages viral RNA genome with nucleic acid (D)</p> Signup and view all the answers

What is the function of integrase (IN) enzyme in retroviruses?

<p>Facilitates integration of viral DNA into host cell DNA (D)</p> Signup and view all the answers

Which proteins are derived from the gag gene in retroviruses?

<p>Matrix, Capsid, Nucleocapsid proteins (A)</p> Signup and view all the answers

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