Questions and Answers
The stage of a microscope contains clips to hold a microscope slide and has a hole in the center called the ______.
aperture
Users can make two types of adjustments on a microscope: coarse adjustment and ______ adjustment.
fine
The diaphragm controls the amount of light that is let through the ______.
aperture
The ______ illuminator is the light source of a microscope.
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A microscope slide typically measures 75 by 26 mm (3 by 1 inches) and about ______ mm thick.
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A prepared slide is typically mounted by a ______ who is a professional in the field.
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Cover slips typically measure between 18 and 25 mm on a side and are ______ to 0.25 mm thick.
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Users should only use the coarse adjustment on the lowest power objective lens, typically the ______ lens.
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A microscope is a scientific instrument used to see things that the naked eye cannot ______.
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The part of the microscope that helps to magnify the object is called the ______.
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The ______ of the microscope is the bottom, solid structure used for support.
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When carrying a microscope, one should hold the arm in one hand and the ______ in the other.
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There are two types of microscope lenses: the objective lens and the ______ lens.
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The typical magnification of the 10x eyepiece lens combined with a 40x objective lens produces an overall magnification of ______.
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The ______ piece holds the objective lenses and helps them rotate.
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The oil immersion lens is unique because it requires special ______ to help focus.
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Always remove oil from the ______ objective lens after its use.
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Keep the stage of the microscope ______ and dry.
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Never force the ______ when using the microscope.
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When not in use, store your microscope in its ______.
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Always incline your head to the side with eyes ______ to the slide.
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Store slides in a dry place with constant room ______ to prevent condensation.
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The main methods of placing samples onto microscope slides are wet mount, dry mount, smear, squash and ______.
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In a dry mount, a thinly sliced section is placed on the center of the slide and a ______ is used to cover the sample.
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Wet mounts are used for aquatic samples, living organisms, and require fluids like water, brine, ______, and immersion oil.
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To prepare a wet mount, first, place a drop of fluid in the ______ of the slide.
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A ______ slide is created by smearing the sample with the edge of one slide onto another.
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To prepare a squash slide, a wet mount is first prepared and then a lens tissue is used to gently ______ down the cover slip.
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When carrying the microscope, use both hands: one grasping the arm, the other beneath the ______.
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Study Notes
Microscope Overview
- A microscope enables observation of objects not visible to the naked eye, revealing details at cellular and atomic levels.
- Special lenses in microscopes enhance the clarity and size of these small structures.
Parts of a Microscope
- Lenses: Magnify the observed object, essential for clarity.
- Head: Located below ocular lenses, houses the revolving nosepiece with internal prisms.
- Base: The solid structure that provides stability and support.
- Arms: Serve as carrying handles connecting the head to the base, used for secure transport.
Microscope Lenses
- Objective Lenses: Positioned below the nosepiece, usually consist of three with varying magnification levels (4x, 10x, 40x, 100x).
- Eyepiece (Ocular Lens): Located at the top, typically offers 10x magnification, enhancing the object's view in conjunction with objective lenses.
- Magnification Calculations: Combining objective and ocular lenses results in magnifications of 40x, 100x, 400x, and 1000x.
Microscope Components
- Nosepiece: Holds and allows rotation of objective lenses.
- Stage: Contains clips for securing slides; features an aperture for light passage.
- Aperture: Adjustable hole for controlling light intensity through specimens.
- Adjustments: Include coarse adjustment for quick focus at low power and fine adjustment for detailed focus.
Slide Usage in Laboratories
- Slides: Standard size approximately 75 x 26 mm; used to hold specimens for microscopic examination.
- Cover Slips: Smaller glass squares used to flatten specimens, enhancing clarity and resolution in viewing.
Prepared Slides
- Prepared Slides: Professionally mounted slides for consistent viewing; must be stored in a dry, stable environment to avoid condensation.
Sample Preparation Techniques
- Dry Mount: Sample placed on a slide, covered by a slip; ideal for solid specimens.
- Wet Mount: Used for aquatic samples; involves suspending specimens in liquids.
- Smear Slides: Produce a thin layer of sample using another slide; appropriate for blood cell observation.
- Squash Slides: A wet mount is gently pressed to view cell division in soft samples.
Microscope Maintenance and Care
- Always carry with both hands; one on the arm and the other on the base.
- Keep lenses clean using lens tissue; avoid touching them.
- Remove slides when not in use; do not leave oil on immersion lenses.
- Ensure stage cleanliness; address spills promptly.
- Proper storage includes lowering the objective lens and securing electrical cords.
- Avoid forced adjustments and improper handling; notify instructors of issues.
- Maintain distance between objective lenses and slides to prevent damage.
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Description
This quiz explores the fundamentals of microscopes, including their purpose and the different types available. Learn about the components that make microscopes essential for viewing microscopic and atomic structures. Test your knowledge on how lenses and other parts contribute to magnification.
