6 Questions
What is the total magnification when using a low-power objective lens with an ocular power of 10x?
60x
Which reagent test is used for detecting proteins and gives a positive result of purple?
Blue and purple reagent
What do the phases of mitosis follow in order from left to right?
Prophase, metaphase, anaphase, telophase
What is the purpose of protease in DNA isolation?
To digest remaining proteins in the cell
Which nitrogenous base is unique to RNA and not found in DNA?
Uracil
Which domain of life includes organisms like plants, animals, and fungi?
Eukarya
Study Notes
The Scientific Method and Microscopy
- The scientific method includes observation, hypothesis, and data analysis
- Indirect evidence is exemplified by observainer
- Total magnification is calculated using the formula: objective x ocular
- When using a microscope, always start with the 10x objective lens to view the slide
Domains of Life
- The three domains of life are eukarya, archaea, and bacteria
Biochemical Tests
- The reagent test with blue and purple liquid tests for proteins, with a positive result indicated by a purple color
- The reagent test with yellow and black results tests for starch, with a positive result indicated by a black color
- The test resulting in multiple colors tests for reducing sugars, with a blue color indicating a negative result
- The test with oil and milk tests for lipids, with a positive result indicated by the presence of lipids
- Veg oil and cream contain lipids, as indicated by the oil and milk test
Cell Biology
- The phases of mitosis, in order, are interphase, prophase, metaphase, anaphase, and telophase
- Skeletal muscle is characterized by pink rope-like shapes under a microscope
- Epithelial cells with nuclei are characterized by purple blubs under a microscope
- White blood cells are characterized by a purple dot under a microscope
- Sister chromatids separate and form in S phase and anaphase
- Alleles are alternative forms of a gene
- Heterozygous dominant (Bb) is not a possible allele pair
DNA Isolation and Structure
- Lysis buffer is used to break open the nucleus in DNA isolation
- Protease is used to digest remaining proteins in the cell during DNA isolation
- Ethanol is used to precipitate DNA during DNA isolation
- DNA is made up of a nitrogenous base, a phosphate group, and a deoxyribose sugar
- RNA nitrogenous bases include adenine, guanine, and uracil
Test your knowledge on the scientific method steps, microscope usage, and biological domains. Questions cover topics like observation, hypothesis, total magnification calculation, and the three domains of life.
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