Scientific Method and Microscope Basics Quiz
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Questions and Answers

What is the total magnification when using a low-power objective lens with an ocular power of 10x?

  • 50x
  • 25x
  • 10x
  • 60x (correct)
  • Which reagent test is used for detecting proteins and gives a positive result of purple?

  • Yellow and black reagent
  • Blue and purple reagent (correct)
  • Test with multiple colors
  • Test with oil and milk
  • What do the phases of mitosis follow in order from left to right?

  • Prophase, metaphase, anaphase, telophase (correct)
  • Interphase, prophase, metaphase, anaphase
  • Anaphase, telophase, prophase, interphase
  • Telophase, anaphase, prophase, metaphase
  • What is the purpose of protease in DNA isolation?

    <p>To digest remaining proteins in the cell</p> Signup and view all the answers

    Which nitrogenous base is unique to RNA and not found in DNA?

    <p>Uracil</p> Signup and view all the answers

    Which domain of life includes organisms like plants, animals, and fungi?

    <p>Eukarya</p> Signup and view all the answers

    Study Notes

    The Scientific Method and Microscopy

    • The scientific method includes observation, hypothesis, and data analysis
    • Indirect evidence is exemplified by observainer
    • Total magnification is calculated using the formula: objective x ocular
    • When using a microscope, always start with the 10x objective lens to view the slide

    Domains of Life

    • The three domains of life are eukarya, archaea, and bacteria

    Biochemical Tests

    • The reagent test with blue and purple liquid tests for proteins, with a positive result indicated by a purple color
    • The reagent test with yellow and black results tests for starch, with a positive result indicated by a black color
    • The test resulting in multiple colors tests for reducing sugars, with a blue color indicating a negative result
    • The test with oil and milk tests for lipids, with a positive result indicated by the presence of lipids
    • Veg oil and cream contain lipids, as indicated by the oil and milk test

    Cell Biology

    • The phases of mitosis, in order, are interphase, prophase, metaphase, anaphase, and telophase
    • Skeletal muscle is characterized by pink rope-like shapes under a microscope
    • Epithelial cells with nuclei are characterized by purple blubs under a microscope
    • White blood cells are characterized by a purple dot under a microscope
    • Sister chromatids separate and form in S phase and anaphase
    • Alleles are alternative forms of a gene
    • Heterozygous dominant (Bb) is not a possible allele pair

    DNA Isolation and Structure

    • Lysis buffer is used to break open the nucleus in DNA isolation
    • Protease is used to digest remaining proteins in the cell during DNA isolation
    • Ethanol is used to precipitate DNA during DNA isolation
    • DNA is made up of a nitrogenous base, a phosphate group, and a deoxyribose sugar
    • RNA nitrogenous bases include adenine, guanine, and uracil

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    Description

    Test your knowledge on the scientific method steps, microscope usage, and biological domains. Questions cover topics like observation, hypothesis, total magnification calculation, and the three domains of life.

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