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Questions and Answers
What role does free coagulase play in the tube coagulase test?
What role does free coagulase play in the tube coagulase test?
Free coagulase reacts with coagulase reacting factor (CRF) in plasma to form thrombin, which converts fibrinogen to fibrin.
What are the key observations that indicate a positive result in the tube coagulase test?
What are the key observations that indicate a positive result in the tube coagulase test?
A positive result is indicated by gelling or clotting of the plasma.
How long should the plasma tubes be observed after incubation for the tube coagulase test?
How long should the plasma tubes be observed after incubation for the tube coagulase test?
Plasma tubes should be observed at intervals of 30 minutes, 1 hour, 2 hours, and up to 4 hours.
What is the importance of using citrated mammalian plasma in the tube coagulase test?
What is the importance of using citrated mammalian plasma in the tube coagulase test?
What would be the interpretation of a negative result in the tube coagulase test?
What would be the interpretation of a negative result in the tube coagulase test?
The free coagulase secreted by S. aureus reacts with coagulase reacting factor (CRF) in plasma to form a complex known as ______.
The free coagulase secreted by S. aureus reacts with coagulase reacting factor (CRF) in plasma to form a complex known as ______.
The procedure involves heavily inoculating a tube containing 0.5 ml citrated mammalian plasma with an unknown ______.
The procedure involves heavily inoculating a tube containing 0.5 ml citrated mammalian plasma with an unknown ______.
After incubation, the plasma tube should be examined for ______ by gently tilting it.
After incubation, the plasma tube should be examined for ______ by gently tilting it.
A positive result in the tube coagulase test shows ______ of the plasma.
A positive result in the tube coagulase test shows ______ of the plasma.
If there is no gelling or clotting in the test, the result is considered ______.
If there is no gelling or clotting in the test, the result is considered ______.
Flashcards
Tube Coagulase Test
Tube Coagulase Test
A test to detect the presence of free coagulase produced by Staphylococcus aureus.
Free Coagulase
Free Coagulase
An enzyme secreted by S. aureus that causes plasma clotting.
Positive Result
Positive Result
Plasma clotting observed in the tube.
Negative Result
Negative Result
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Incubation Time
Incubation Time
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What causes plasma clotting in the tube coagulase test?
What causes plasma clotting in the tube coagulase test?
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What is a positive result in the tube coagulase test?
What is a positive result in the tube coagulase test?
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What is a negative result in the tube coagulase test?
What is a negative result in the tube coagulase test?
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Why is citrated plasma used?
Why is citrated plasma used?
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What's the role of the coagulase reacting factor (CRF)?
What's the role of the coagulase reacting factor (CRF)?
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Study Notes
Tube Coagulase Test
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Principle: Staphylococcus aureus secretes free coagulase, which reacts with plasma's coagulase reacting factor (CRF) to form a clot. This process converts fibrinogen to fibrin.
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Procedure:
- Inoculate 0.5 ml of citrated mammalian plasma with the unknown bacteria.
- Incubate at 37°C.
- Observe the tube for clotting at 30 minutes, 1 hour, 2 hours, and up to 4 hours, gently tilting.
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Interpretation:
- Positive: Plasma clots (gelling) – indicates Staphylococcus aureus.
- Negative: No clotting (no gelling) – indicates Staphylococcus epidermidis.
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