Podcast
Questions and Answers
What role does free coagulase play in the tube coagulase test?
What role does free coagulase play in the tube coagulase test?
Free coagulase reacts with coagulase reacting factor (CRF) in plasma to form thrombin, which converts fibrinogen to fibrin.
What are the key observations that indicate a positive result in the tube coagulase test?
What are the key observations that indicate a positive result in the tube coagulase test?
A positive result is indicated by gelling or clotting of the plasma.
How long should the plasma tubes be observed after incubation for the tube coagulase test?
How long should the plasma tubes be observed after incubation for the tube coagulase test?
Plasma tubes should be observed at intervals of 30 minutes, 1 hour, 2 hours, and up to 4 hours.
What is the importance of using citrated mammalian plasma in the tube coagulase test?
What is the importance of using citrated mammalian plasma in the tube coagulase test?
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What would be the interpretation of a negative result in the tube coagulase test?
What would be the interpretation of a negative result in the tube coagulase test?
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The free coagulase secreted by S. aureus reacts with coagulase reacting factor (CRF) in plasma to form a complex known as ______.
The free coagulase secreted by S. aureus reacts with coagulase reacting factor (CRF) in plasma to form a complex known as ______.
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The procedure involves heavily inoculating a tube containing 0.5 ml citrated mammalian plasma with an unknown ______.
The procedure involves heavily inoculating a tube containing 0.5 ml citrated mammalian plasma with an unknown ______.
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After incubation, the plasma tube should be examined for ______ by gently tilting it.
After incubation, the plasma tube should be examined for ______ by gently tilting it.
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A positive result in the tube coagulase test shows ______ of the plasma.
A positive result in the tube coagulase test shows ______ of the plasma.
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If there is no gelling or clotting in the test, the result is considered ______.
If there is no gelling or clotting in the test, the result is considered ______.
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Study Notes
Tube Coagulase Test
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Principle: Staphylococcus aureus secretes free coagulase, which reacts with plasma's coagulase reacting factor (CRF) to form a clot. This process converts fibrinogen to fibrin.
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Procedure:
- Inoculate 0.5 ml of citrated mammalian plasma with the unknown bacteria.
- Incubate at 37°C.
- Observe the tube for clotting at 30 minutes, 1 hour, 2 hours, and up to 4 hours, gently tilting.
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Interpretation:
- Positive: Plasma clots (gelling) – indicates Staphylococcus aureus.
- Negative: No clotting (no gelling) – indicates Staphylococcus epidermidis.
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Description
This quiz covers the Tube Coagulase Test, a diagnostic procedure used to identify Staphylococcus aureus through its ability to convert fibrinogen to fibrin. Participants will learn about the principle, procedure, and interpretation of results. Test your knowledge on key aspects of this important microbiological technique.
