Translation Initiation Process Quiz

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Questions and Answers

What is the role of the Shine-Dalgarno sequence in translation initiation?

  • It binds to release factors during termination.
  • It enhances the dissociation of the large and small ribosomal subunits.
  • It forms a complex with GTP during elongation.
  • It pairs with their complementary nucleotides in the 16S rRNA. (correct)

What is the function of initiation factor 3 (IF-3) during translation initiation?

  • Binds to the small subunit and prevents binding of the large subunit. (correct)
  • Forms a complex with initiator tRNA.
  • Facilitates translocation of the ribosome.
  • Enhances the dissociation of the large and small ribosomal subunits.

In eukaryotic cells, what plays a critical role in the initiation of protein synthesis?

  • The cap at the 3' end of mRNA.
  • The Shine-Dalgarno sequence on mRNA.
  • The cap at the 5' end of mRNA. (correct)
  • The presence of initiator tRNA.

During elongation, what is the role of elongation factor Tu (EF-Tu)?

<p>Facilitates delivery of a charged tRNA to the A site. (D)</p> Signup and view all the answers

What triggers termination of protein synthesis?

<p>Encounter of a termination codon by the ribosome. (A)</p> Signup and view all the answers

Which factor prevents the large subunit of the ribosome from binding during translation initiation?

<p>Initiation factor 2 (IF-2) (C)</p> Signup and view all the answers

What is the first step in the maturation of mRNA in eukaryotes?

<p>Capping the 5'-end with a guanosine triphosphate (D)</p> Signup and view all the answers

What is the function of the Poly(A) tail in eukaryotic mRNA?

<p>Recognition of the 3' end (B)</p> Signup and view all the answers

Which enzyme is responsible for adding a guanosine triphosphate to the 5'-end of mRNA?

<p>Capping enzyme (B)</p> Signup and view all the answers

What sequence is recognized by a specific endonuclease for cutting the growing RNA molecule in eukaryotic mRNA?

<p>AAUAAA (A)</p> Signup and view all the answers

What happens after recognition and cutting of the growing RNA molecule at AAUAAA sequence?

<p>Poly(A) tail is added (B)</p> Signup and view all the answers

What modification occurs to the guanine of the cap on eukaryotic mRNA?

<p>Methylation at the 7 position (B)</p> Signup and view all the answers

What is the significance of having multiple isoaccepting tRNAs in cells?

<p>It prevents the binding of incorrect amino acids to tRNAs. (D)</p> Signup and view all the answers

Which hypothesis explains the ability of some non-standard base pairings at the third position of a codon?

<p>Wobble hypothesis (A)</p> Signup and view all the answers

What is the role of aminoacyl-tRNA synthetases in protein synthesis?

<p>They mediate the specific attachment of amino acids to tRNAs. (B)</p> Signup and view all the answers

Where does a ribosome attach during translation on an mRNA molecule?

<p>At the 5' UTR region (D)</p> Signup and view all the answers

What provides the energy required for the attachment of tRNA to its appropriate amino acid?

<p>ATP (D)</p> Signup and view all the answers

Which part of a tRNA molecule is responsible for attaching to its corresponding amino acid?

<p>'CCA' sequence at the 3' end (C)</p> Signup and view all the answers

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