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Questions and Answers
What is the biolistic method used for in plant genetic modification?
Which natural plant parasite is used as a vector for transporting foreign genes into plants?
What is the purpose of genetically modifying plants to produce vaccines in their fruit?
What is the main application of transgenesis in biotechnology?
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Which method is used to integrate DNA into the plant genome inside the nucleus?
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What is the method used for creation of transgenic animals through introduction of foreign DNA?
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Which technique involves transplantation of living cells, tissues, or organs from one species to another?
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What is the first FDA-approved transgenically produced therapeutic protein?
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What method is used to produce genetically modified plants by altering their genetic makeup?
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Which method has a high frequency of generating transgenic animals and lacks constraints on DNA constructs?
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What is the potential drawback of retrovirus-mediated gene transfer?
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Which method involves the establishment of ova culture media for embryo manipulation?
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What is the use of fine glass microinjection pipette primarily associated with in this context?
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What does xenotransplantation involve overcoming?
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What is the differentiation between cloned and transgenic animals related to?
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What is the term 'transgenic animal' coined to describe?
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What is one of the requirements for creating a transgenic animal?
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What is the role of a promoter and stop codons in recombinant DNA technology?
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What is the purpose of microinjection in transgenic animal technology?
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What can be done using recombinant DNA technology in transgenic animals?
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What can be selectively removed from the host genome in transgenic animals?
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Which technology involves using a 'DNA vector' to enable insertion into the host DNA?
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'Knockout' mice are produced by selectively removing which of the following from the host genome?
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'Transgenic' has been extended to include what type of experimentation in animals?
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Study Notes
- Use of fine glass microinjection pipette for gene delivery into embryos
- In vitro manipulation of fertilized eggs requires proper culture system for embryo viability
- Establishment of ova culture media for embryo manipulation
- Derivation of embryonic stem cells (blastocysts) from early preimplantation embryos for in vitro manipulations
- Creation of transgenic animals through DNA microinjection, retrovirus-mediated gene transfer, or cloning
- DNA microinjection: high frequency of generating transgenic animals, lack of constraint on DNA constructs, stable transgene transmission, random integration of gene, potential for undesired insertional mutagenesis, variation in gene expression levels, time and expense requirement
- Retrovirus-mediated gene transfer: high gene transfer efficiency, limitations on foreign DNA size, potential for undesired genetic recombination, production difficulties
- Transgenic animals used as lab models for human disease, production of pharmaceuticals, and xenotransplantation
- Transgenic animals used in lab to study human diseases, therapeutic strategies for Alzheimer's disease
- Production of therapeutic proteins from transgenic animals, first FDA-approved transgenically produced therapeutic protein (ATryn)
- Xenotransplantation: transplantation of living cells, tissues, or organs from one species to another, immunological barriers leading to rejection, methods to overcome immunological barriers through pig modifications
- Cloning as alternative breeding methodology, difference between cloned and transgenic animals
- Genetically modified plants created through laboratories by altering their genetic makeup, most commonly through biolistic method or Agrobacterium tumefaciens bacteria.
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Description
Test your knowledge of transgenic animals, including the definition of transgenesis, the process of creating transgenic animals, the applications of transgenesis, and the differences between transgenic and cloned animals. Explore the history and significance of transgenic animals and plants.
