Transgenic Animals Quiz

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Questions and Answers

What is the biolistic method used for in plant genetic modification?

Generating transgenic animals

Cloning plants for medical and pharmaceutical use

Introducing DNA into plant tissue using accelerated particles (correct)

Producing vaccines in plant fruit

Which natural plant parasite is used as a vector for transporting foreign genes into plants?

Tungsten

Betacarotene

Polio vaccine

Agrobacteria (correct)

What is the purpose of genetically modifying plants to produce vaccines in their fruit?

To enable plants to produce vaccines like the polio vaccine (correct)

To make plants resistant to disease and pests

To reduce the need for pesticides and herbicides

To increase the nutritional value of plants

What is the main application of transgenesis in biotechnology?

Generating transgenic animals for medical and pharmaceutical use Signup and view all the answers

Which method is used to integrate DNA into the plant genome inside the nucleus?

Biolistic method Signup and view all the answers

What is the method used for creation of transgenic animals through introduction of foreign DNA?

Retrovirus-mediated gene transfer Signup and view all the answers

Which technique involves transplantation of living cells, tissues, or organs from one species to another?

Xenotransplantation Signup and view all the answers

What is the first FDA-approved transgenically produced therapeutic protein?

ATryn Signup and view all the answers

What method is used to produce genetically modified plants by altering their genetic makeup?

Biolistic method Signup and view all the answers

Which method has a high frequency of generating transgenic animals and lacks constraints on DNA constructs?

DNA microinjection Signup and view all the answers

What is the potential drawback of retrovirus-mediated gene transfer?

Potential for undesired genetic recombination Signup and view all the answers

Which method involves the establishment of ova culture media for embryo manipulation?

In vitro manipulation of fertilized eggs Signup and view all the answers

What is the use of fine glass microinjection pipette primarily associated with in this context?

DNA microinjection Signup and view all the answers

What does xenotransplantation involve overcoming?

Immunological barriers Signup and view all the answers

What is the differentiation between cloned and transgenic animals related to?

Breeding methodology Signup and view all the answers

What is the term 'transgenic animal' coined to describe?

An animal with an exogenous gene introduced into its genome Signup and view all the answers

What is one of the requirements for creating a transgenic animal?

Using recombinant DNA technology Signup and view all the answers

What is the role of a promoter and stop codons in recombinant DNA technology?

To enable the gene to be expressed in the host cells Signup and view all the answers

What is the purpose of microinjection in transgenic animal technology?

To insert the recombinant DNA into the host DNA Signup and view all the answers

What can be done using recombinant DNA technology in transgenic animals?

Inserting extra genes (transgenes) Signup and view all the answers

What can be selectively removed from the host genome in transgenic animals?

Gene (or genes) Signup and view all the answers

Which technology involves using a 'DNA vector' to enable insertion into the host DNA?

Recombinant DNA technology Signup and view all the answers

'Knockout' mice are produced by selectively removing which of the following from the host genome?

Gene (or genes) Signup and view all the answers

'Transgenic' has been extended to include what type of experimentation in animals?

'Gene-targeting' experimentation Signup and view all the answers

Study Notes

Use of fine glass microinjection pipette for gene delivery into embryos
In vitro manipulation of fertilized eggs requires proper culture system for embryo viability
Establishment of ova culture media for embryo manipulation
Derivation of embryonic stem cells (blastocysts) from early preimplantation embryos for in vitro manipulations
Creation of transgenic animals through DNA microinjection, retrovirus-mediated gene transfer, or cloning
DNA microinjection: high frequency of generating transgenic animals, lack of constraint on DNA constructs, stable transgene transmission, random integration of gene, potential for undesired insertional mutagenesis, variation in gene expression levels, time and expense requirement
Retrovirus-mediated gene transfer: high gene transfer efficiency, limitations on foreign DNA size, potential for undesired genetic recombination, production difficulties
Transgenic animals used as lab models for human disease, production of pharmaceuticals, and xenotransplantation
Transgenic animals used in lab to study human diseases, therapeutic strategies for Alzheimer's disease
Production of therapeutic proteins from transgenic animals, first FDA-approved transgenically produced therapeutic protein (ATryn)
Xenotransplantation: transplantation of living cells, tissues, or organs from one species to another, immunological barriers leading to rejection, methods to overcome immunological barriers through pig modifications
Cloning as alternative breeding methodology, difference between cloned and transgenic animals
Genetically modified plants created through laboratories by altering their genetic makeup, most commonly through biolistic method or Agrobacterium tumefaciens bacteria.

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Description

Test your knowledge of transgenic animals, including the definition of transgenesis, the process of creating transgenic animals, the applications of transgenesis, and the differences between transgenic and cloned animals. Explore the history and significance of transgenic animals and plants.