Podcast
Questions and Answers
What is the main aim of animal breeders in enhancing productive performance traits?
What is the main aim of animal breeders in enhancing productive performance traits?
- To eliminate all genetic disorders in animals
- To create entirely new species of animals
- To increase the lifespan of domestic animals
- To improve growth, milk yield, and feeding efficiency (correct)
Which of the following describes transgenesis?
Which of the following describes transgenesis?
- The observation of inherited characteristics to determine genetics
- The process of using spontaneous mutations to improve traits
- Insertion of cDNA into cells through human intervention (correct)
- Manipulation of an animal's phenotype using selective breeding
What is targeted mutation in the context of genetic engineering?
What is targeted mutation in the context of genetic engineering?
- It refers to making a specific gene nonfunctional or functional. (correct)
- It requires the addition of new species to the gene pool.
- It is a method for enhancing the growth rate of animals.
- It involves introducing random genes into the genome.
Which statement best describes the current routine use of transgenic animals?
Which statement best describes the current routine use of transgenic animals?
What is one of the contributions of Developmental Biology and Genetic Engineering?
What is one of the contributions of Developmental Biology and Genetic Engineering?
What is one potential agricultural application of genetic engineering in animals?
What is one potential agricultural application of genetic engineering in animals?
Before the advent of molecular genetics, how was the regulation and function of mammalian genes understood?
Before the advent of molecular genetics, how was the regulation and function of mammalian genes understood?
Which option best characterizes the outcomes of DNA transfer methods in genetic engineering?
Which option best characterizes the outcomes of DNA transfer methods in genetic engineering?
What defines a transgenic animal?
What defines a transgenic animal?
Which of the following was the first transgenic organism?
Which of the following was the first transgenic organism?
What is the primary method used for creating transgenic animals?
What is the primary method used for creating transgenic animals?
Why is it challenging to create functional transgenic animals?
Why is it challenging to create functional transgenic animals?
Which of the following describes one of the strategies in producing transgenic animals?
Which of the following describes one of the strategies in producing transgenic animals?
What must accompany the gene to be transferred for it to function correctly?
What must accompany the gene to be transferred for it to function correctly?
Which of the following is NOT a method used in the production of transgenic animals?
Which of the following is NOT a method used in the production of transgenic animals?
What application does transgenic animal technology primarily focus on?
What application does transgenic animal technology primarily focus on?
Flashcards
Transgenic animal
Transgenic animal
An animal altered by adding recombinant DNA.
Transgenesis
Transgenesis
Inserting cDNA into animal cells.
Targeted mutation
Targeted mutation
Making a specific gene non-functional or less functional.
Selective breeding
Selective breeding
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Molecular genetics
Molecular genetics
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Genetic improvement
Genetic improvement
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DNA transfer methods
DNA transfer methods
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Phenotypic selective breeding
Phenotypic selective breeding
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Recombinant DNA
Recombinant DNA
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Transgene expression
Transgene expression
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Transgenic animal production methods
Transgenic animal production methods
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Promoter and regulatory sequences
Promoter and regulatory sequences
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Production trait enhancement
Production trait enhancement
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Commercial product biosynthesis
Commercial product biosynthesis
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First transgenic organism
First transgenic organism
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Study Notes
Selective Breeding and Transgenic Animals
- Animal breeders have long sought to improve traits like growth, milk yield, and feeding efficiency in farm animals.
- Phenotypic selective breeding, enhancing superior genetic traits, increased domestic animal productivity.
- Before molecular genetics, genetic regulation & function were observed through inherited characteristics or spontaneous mutations.
- Selective breeding was a pre-existing practice for trait enhancement.
Defining Transgenic Animals
- Transgenic animal: An animal altered by the introduction of recombinant DNA through human intervention.
- Transgenesis: Insertion of cDNA (from mRNA) into animal cells, directly manipulating DNA for gene expression.
- Developmental Biology and Genetic Engineering facilitated transgenic animal creation.
- Early gene transfers into mice showed foreign DNA integration & phenotypic modification was achievable.
DNA Transfer Methods and Applications
- Some DNA transfer methods cause random gene addition, targeted gene integration (homologous recombination/gene replacement), and mutation.
- Targeted mutation: Making a gene nonfunctional (knock-out) or less functional (knock-in).
- Current routine use of transgenic animals (mostly mice) is in human disease research.
- Potential agricultural applications include improved feed use, faster growth, disease resistance, leaner meat/other desirable qualities, and environmentally friendly animal waste.
Transgenic Animal Production & Design
- Transgenic animals carry a foreign gene (recombinant DNA) in their genome.
- Transgenic DNA usually includes sequences for incorporation & expression by host cells.
- Transgenic animals are modified to enhance traits.
- The first transgenic organism was E. coli, followed by a mouse with SV-40 viral DNA. SV-40 transgene was not expressed. The first expressing transgene animal was a mouse with cloned growth hormone.
Methods for Transgenic Livestock Production
- Various methods exist for producing transgenic livestock, including micro-injection, embryonic stem-cell, retrovirus, sperm-mediated, transposon-mediated, and nuclear transfer.
Ensuring Transgene Functionality
- For a transgene to function properly, integration of the appropriate promoter and regulatory sequences is needed.
- Some genes require an enhancer, potentially located away from the promoter.
- Correct fusion of promoters/enhancers is important for targeted gene expression.
Transgenic Animal Strategies
- Enhancing animal value for production traits and conventional animal products (e.g., milk, meat).
- Generating animals for biosynthesizing non-conventional commercial products.
Overall Applications
- Transgenic animal technology: Identifying/manipulating genes and their applications in domesticated farm animals.
- Improve animal productivity.
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Description
Explore the concepts of selective breeding and the creation of transgenic animals. Understand how genetic engineering has influenced animal traits and productivity through various DNA transfer methods. This quiz will test your knowledge on these fundamental biotechnological practices.