Transformation in Bacteria Quiz
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Questions and Answers

Which scientist published the double helical model of the DNA structure in 1953?

  • Hershey & Chase
  • Erwin Chargaff
  • Watson & Crick (correct)
  • Phage T2

    • What is the main reason protein is considered rich in nitrogen?

  • High nitrogen content (correct)
  • Low hydrogen content
  • High phosphorus content
  • Low sulfur content

    • What is the function of a nucleoside in DNA?

  • Initiate protein synthesis
  • Form sugar-phosphate backbone
  • Provide energy for cellular processes
  • Carry genetic information (correct)

    • What was the organism used in the Hershey-Chase experiment to study DNA replication?

    <p>Phage T2</p> Signup and view all the answers

    What did Erwin Chargaff's research reveal about DNA bases?

    <p>Variation among different species</p> Signup and view all the answers

    What was the transforming material identified as in the 1944 experiment conducted by Oswald Avery, Colin MacLeod, and Maclyn McCarty?

    <p>DNA</p> Signup and view all the answers

    Which analytical tool was used to determine the high molecular weight of the transforming material?

    <p>Ultracentrifugation</p> Signup and view all the answers

    What characteristic of DNA helped differentiate it from proteins in the experiment?

    <p>Nitrogen/phosphorus ratio</p> Signup and view all the answers

    Which wavelength of light absorption is most strongly associated with DNA?

    <p>260 nm</p> Signup and view all the answers

    What did the average nitrogen/phosphorus ratio of 1.67 suggest about the transforming material?

    <p>It's DNA</p> Signup and view all the answers

    Which analytical tool was used to match the absorption spectrum of the transforming material to that of DNA?

    <p>UV absorption spectrophotometry</p> Signup and view all the answers

    Which technique did Maurice Wilkins and Rosalind Franklin use to analyze the three-dimensional structure of DNA?

    <p>X-ray diffraction</p> Signup and view all the answers

    What does the arrangement of spots in an X-shape in X-ray diffraction analysis of DNA indicate?

    <p>Simple structure</p> Signup and view all the answers

    Why is DNA considered simple when analyzed using X-ray diffraction?

    <p>Its large size implies a regular repeating structure</p> Signup and view all the answers

    According to Crick and Watson, why are purines paired with pyrimidines in DNA?

    <p>To ensure uniform base pairs in double-stranded DNA</p> Signup and view all the answers

    What is the significance of the spacing between adjacent spots in the X-ray diffraction pattern of DNA?

    <p>Correlates with overall helix length</p> Signup and view all the answers

    How did Rosalind Franklin's X-ray results contribute to understanding DNA?

    <p>Gave information about DNA size and shape</p> Signup and view all the answers

    Which of the following nitrogenous bases is found in DNA but not in RNA?

    <p>Thymine</p> Signup and view all the answers

    What is the major structural difference between ribose and deoxyribose sugars?

    <p>Deoxyribose has an oxygen atom on the 2nd carbon atom while ribose doesn't.</p> Signup and view all the answers

    Which of the following statements is true about nucleotides?

    <p>Thymidic acid is a pyrimidine nucleotide.</p> Signup and view all the answers

    What structural feature gives nucleotide chains polarity?

    <p>Phosphate group location</p> Signup and view all the answers

    Which scientist was known for discovering the Chargaff's rules for DNA base pairing?

    <p>Erwin Chargaff</p> Signup and view all the answers

    What does Chargaff's rule regarding DNA structure state?

    <p>'A+T' equals 'G+C'</p> Signup and view all the answers

    What is the correct number of hydrogen bonds between adenine and thymine in DNA?

    <p>2 hydrogen bonds</p> Signup and view all the answers

    What type of bonding occurs between guanine and cytosine in DNA?

    <p>Hydrogen bonding</p> Signup and view all the answers

    What is the distance between base pairs in the DNA molecule?

    <p>3.4 Å</p> Signup and view all the answers

    How many base pairs are there approximately per turn of the DNA helix?

    <p>10 base pairs</p> Signup and view all the answers

    What is the approximate diameter of the DNA molecule?

    <p>20 Å</p> Signup and view all the answers

    In DNA, why must one strand have a 5'-3' polarity if the other has a 3'-5’ polarity?

    <p>To allow for complementary base pairing</p> Signup and view all the answers

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