Transcription Process in Prokaryotes
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Questions and Answers

What is the role of the σ subunit in RNA transcription?

  • To unwind DNA and form a transcription bubble
  • To recognize the terminator sequence
  • To elongate the newly synthesized RNA strand
  • To recognize the promoter region to initiate RNA synthesis (correct)

What occurs at the +1 site during RNA transcription?

  • The RNA polymerase holoenzyme binds nonspecifically to DNA
  • The transcription bubble is formed and the σ subunit leaves (correct)
  • The σ subunit recognizes the promoter region
  • The RNA polymerase core enzyme elongates the RNA strand

What is the function of TFIIH in the transcription process?

  • It initiates the transcription process
  • It phosphorylates the CTD of RNA polymerase II (correct)
  • It enhances the rate of transcription
  • It binds to the TBP

What is the function of the core enzyme in RNA transcription?

<p>To elongate the newly synthesized RNA strand (A)</p> Signup and view all the answers

What is the purpose of the 5' cap in mRNA processing?

<p>To stabilize the mRNA (C)</p> Signup and view all the answers

What type of terminator sequence is characterized by a hairpin loop followed by a sequence of U's?

<p>ρ-independent (D)</p> Signup and view all the answers

What is the role of ρ in terminator sequences?

<p>To destabilize the DNA-RNA hybrid in the transcription bubble (C)</p> Signup and view all the answers

What is the consensus sequence for polyadenylation?

<p>AAUAAA (C)</p> Signup and view all the answers

What is the function of the spliceosome in mRNA processing?

<p>To remove introns and join exons (B)</p> Signup and view all the answers

What is the composition of the RNA polymerase holoenzyme?

<p>Core enzyme + σ subunit (B)</p> Signup and view all the answers

What is the purpose of the 2' OH group in the branch site of the intron?

<p>To facilitate splicing (A)</p> Signup and view all the answers

What is the result of the coupling of transcription and post-transcription?

<p>The formation of mature mRNA (A)</p> Signup and view all the answers

What is the function of the σ subunit of holoenzyme during RNA transcription?

<p>To recognize the Pribnow box and –35 region (D)</p> Signup and view all the answers

What is the difference between the coding strand and the template strand of DNA?

<p>The coding strand is identical to the mRNA strand, except U in mRNA is replaced by T (A)</p> Signup and view all the answers

What is the function of tRNA during protein synthesis?

<p>To synthesize protein from mRNA (B)</p> Signup and view all the answers

What is the direction of transcription during RNA synthesis?

<p>5' → 3' (C)</p> Signup and view all the answers

What is the function of the Pribnow box and –35 region during RNA transcription?

<p>To recognize the promoter region by σ (B)</p> Signup and view all the answers

What is the difference between rho-dependent and rho-independent termination?

<p>Rho-dependent termination requires a protein, while rho-independent termination does not (C)</p> Signup and view all the answers

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