Podcast
Questions and Answers
Where does transcription occur in prokaryotes?
Where does transcription occur in prokaryotes?
- Nucleus
- Cytoplasm (correct)
- Mitochondria
- Nucleolus
What is the direction of RNA synthesis during transcription in prokaryotes?
What is the direction of RNA synthesis during transcription in prokaryotes?
- 5'-3' (correct)
- 3'-5'
- 5'-5'
- 3'-3'
What is the function of the promoter site in prokaryotic transcription?
What is the function of the promoter site in prokaryotic transcription?
- Unwinds the DNA strand and initiates transcription (correct)
- Unwinds the DNA strand and terminates transcription
- Winds the DNA strand and terminates transcription
- Winds the DNA strand and initiates transcription
What is the role of the 3'-OH group of the growing RNA chain during transcription?
What is the role of the 3'-OH group of the growing RNA chain during transcription?
What triggers the termination of transcription in prokaryotes?
What triggers the termination of transcription in prokaryotes?
What is the purpose of the promoter sequence during transcription in prokaryotes?
What is the purpose of the promoter sequence during transcription in prokaryotes?
What is the template for RNA synthesis during transcription in prokaryotes?
What is the template for RNA synthesis during transcription in prokaryotes?
What type of bond is formed between the incoming nucleotide and the growing RNA chain during transcription?
What type of bond is formed between the incoming nucleotide and the growing RNA chain during transcription?
What happens to the RNA polymerase when it detects a termination sequence on the DNA?
What happens to the RNA polymerase when it detects a termination sequence on the DNA?
What is the main enzyme responsible for transcription in prokaryotes?
What is the main enzyme responsible for transcription in prokaryotes?
Study Notes
Transcription in Prokaryotes
- Transcription occurs in the cytoplasm in prokaryotes
- The process of transcription consists of three phases: initiation, elongation, and termination
Initiation Phase
- RNA polymerase recognizes a promoter site on the DNA strand during initiation
- The promoter sequence is read, unwinding the DNA strand and initiating transcription
- The core enzyme of RNA polymerase then proceeds to synthesize RNA in the 5'-3' direction
RNA Synthesis
- The antisense strand of DNA is used as the template for RNA synthesis
- The 3'-OH group of the growing RNA chain interacts with the α phosphate group of the incoming nucleotide to form a phosphodiester bond
Termination Phase
- Transcription occurs until a termination sequence on the DNA is found
- When the RNA polymerase detects a termination sequence, it stops the transcription process
- The synthesized RNA transcript is released, and the RNA polymerase dissociates from the DNA
Transcription in Prokaryotes
- Transcription occurs in the cytoplasm in prokaryotes
- The process of transcription consists of three phases: initiation, elongation, and termination
Initiation Phase
- RNA polymerase recognizes a promoter site on the DNA strand during initiation
- The promoter sequence is read, unwinding the DNA strand and initiating transcription
- The core enzyme of RNA polymerase then proceeds to synthesize RNA in the 5'-3' direction
RNA Synthesis
- The antisense strand of DNA is used as the template for RNA synthesis
- The 3'-OH group of the growing RNA chain interacts with the α phosphate group of the incoming nucleotide to form a phosphodiester bond
Termination Phase
- Transcription occurs until a termination sequence on the DNA is found
- When the RNA polymerase detects a termination sequence, it stops the transcription process
- The synthesized RNA transcript is released, and the RNA polymerase dissociates from the DNA
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Description
This quiz covers the process of transcription in prokaryotes, including the three phases of initiation, elongation, and termination, and the role of RNA polymerase.
