10 Questions
Where does transcription occur in prokaryotes?
Cytoplasm
What is the direction of RNA synthesis during transcription in prokaryotes?
5'-3'
What is the function of the promoter site in prokaryotic transcription?
Unwinds the DNA strand and initiates transcription
What is the role of the 3'-OH group of the growing RNA chain during transcription?
Interacts with the α phosphate group of the incoming nucleotide to form a phosphodiester bond
What triggers the termination of transcription in prokaryotes?
When the RNA polymerase detects a termination sequence
What is the purpose of the promoter sequence during transcription in prokaryotes?
To unwind the DNA strand and initiate transcription
What is the template for RNA synthesis during transcription in prokaryotes?
The antisense strand of DNA
What type of bond is formed between the incoming nucleotide and the growing RNA chain during transcription?
Phosphodiester bond
What happens to the RNA polymerase when it detects a termination sequence on the DNA?
It stops transcription and releases the RNA transcript
What is the main enzyme responsible for transcription in prokaryotes?
RNA polymerase
Study Notes
Transcription in Prokaryotes
- Transcription occurs in the cytoplasm in prokaryotes
- The process of transcription consists of three phases: initiation, elongation, and termination
Initiation Phase
- RNA polymerase recognizes a promoter site on the DNA strand during initiation
- The promoter sequence is read, unwinding the DNA strand and initiating transcription
- The core enzyme of RNA polymerase then proceeds to synthesize RNA in the 5'-3' direction
RNA Synthesis
- The antisense strand of DNA is used as the template for RNA synthesis
- The 3'-OH group of the growing RNA chain interacts with the α phosphate group of the incoming nucleotide to form a phosphodiester bond
Termination Phase
- Transcription occurs until a termination sequence on the DNA is found
- When the RNA polymerase detects a termination sequence, it stops the transcription process
- The synthesized RNA transcript is released, and the RNA polymerase dissociates from the DNA
Transcription in Prokaryotes
- Transcription occurs in the cytoplasm in prokaryotes
- The process of transcription consists of three phases: initiation, elongation, and termination
Initiation Phase
- RNA polymerase recognizes a promoter site on the DNA strand during initiation
- The promoter sequence is read, unwinding the DNA strand and initiating transcription
- The core enzyme of RNA polymerase then proceeds to synthesize RNA in the 5'-3' direction
RNA Synthesis
- The antisense strand of DNA is used as the template for RNA synthesis
- The 3'-OH group of the growing RNA chain interacts with the α phosphate group of the incoming nucleotide to form a phosphodiester bond
Termination Phase
- Transcription occurs until a termination sequence on the DNA is found
- When the RNA polymerase detects a termination sequence, it stops the transcription process
- The synthesized RNA transcript is released, and the RNA polymerase dissociates from the DNA
This quiz covers the process of transcription in prokaryotes, including the three phases of initiation, elongation, and termination, and the role of RNA polymerase.
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