Transcription, Enzymes, and Limiting Factors
54 Questions
8 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

According to the law of limiting factors, if a reaction depends on multiple conditions, what determines the reaction rate?

  • The factor nearest to its minimum value. (correct)
  • The average of all essential conditions.
  • The sum of all essential conditions.
  • The factor present in the highest concentration.
  • Which of the following cellular processes is NOT directly mentioned as being dependent on specific environmental conditions?

  • DNA replication
  • Transcription
  • Translation (correct)
  • Cell respiration
  • What is the primary function of RNA polymerase during transcription?

  • To synthesize proteins from RNA transcripts.
  • To initiate DNA replication by adding complementary DNA nucleotides.
  • To position RNA nucleotides and link them to form a continuous RNA strand. (correct)
  • To unwind the entire DNA molecule for replication.
  • In the process of transcription, what dictates the sequence of the newly synthesized RNA molecule?

    <p>Complementary base pairing with the template DNA strand. (B)</p> Signup and view all the answers

    If a template DNA strand has the sequence 3'-TTCAGTC-5', what would be the corresponding RNA sequence produced during transcription?

    <p><code>5'-AAGUCAA-3'</code> (D)</p> Signup and view all the answers

    Which of the following BEST describes the role of the 'sense strand' (or 'coding strand') in transcription?

    <p>It is identical in sequence to the synthesized RNA, except with uracil replacing thymine. (D)</p> Signup and view all the answers

    What event signals the termination of transcription?

    <p>RNA polymerase encounters a specific sequence indicating the end of the gene. (D)</p> Signup and view all the answers

    What is the relationship between a gene and an RNA transcript?

    <p>A gene is a sequence of DNA that is transcribed into RNA. (A)</p> Signup and view all the answers

    How does a conformational change in an enzyme promote catalytic activity?

    <p>By stressing bonds in the substrate, thus lowering the activation energy. (C)</p> Signup and view all the answers

    Which of the following methods would NOT effectively measure enzyme activity?

    <p>Measuring the total enzyme concentration in the solution. (A)</p> Signup and view all the answers

    An enzyme-catalyzed reaction initially proceeds slowly. Which factor change is most likely to increase the reaction rate?

    <p>Increasing the substrate concentration significantly. (C)</p> Signup and view all the answers

    How does increasing the level of molecular motion affect enzyme activity?

    <p>It increases the frequency of successful collisions between enzyme and substrate. (D)</p> Signup and view all the answers

    What is the direct effect of enzyme denaturation on enzyme activity?

    <p>It alters the enzyme's active site, hindering substrate binding. (D)</p> Signup and view all the answers

    How does changing the pH affect enzyme activity?

    <p>It alters the charge of the enzyme, affecting both its solubility and shape. (C)</p> Signup and view all the answers

    At a certain substrate concentration, the rate of an enzyme-catalyzed reaction reaches a plateau. Why does this occur?

    <p>All active sites are occupied by the substrate. (A)</p> Signup and view all the answers

    In photosynthesis, what is the role of light energy?

    <p>To synthesize organic compounds from carbon dioxide and water. (A)</p> Signup and view all the answers

    How do absorption spectra and action spectra differ in the context of photosynthesis?

    <p>Absorption spectra identify wavelengths absorbed by photosynthetic pigments, whereas action spectra indicate wavelengths effectively used in photosynthesis. (C)</p> Signup and view all the answers

    In chromatography, what determines the separation of photosynthetic pigments?

    <p>The pigments' differing affinities for the mobile and stationary phases. (A)</p> Signup and view all the answers

    Which of the following wavelength ranges corresponds to visible light used in photosynthesis?

    <p>400nm to 700nm (B)</p> Signup and view all the answers

    What is the primary role of light-dependent reactions in photosynthesis?

    <p>To convert light energy into chemical energy in the form of ATP. (A)</p> Signup and view all the answers

    Why do plants appear green?

    <p>Chlorophyll absorbs red and blue light, reflecting/transmitting green light. (C)</p> Signup and view all the answers

    How do light-dependent reactions contribute to the subsequent light-independent reactions?

    <p>By producing the ATP and hydrogen required to fix carbon. (B)</p> Signup and view all the answers

    What is the role of ATP in the light-independent reactions? Choose the best answer.

    <p>It provides the energy required to fix carbon dioxide and synthesize new organic compounds. (B)</p> Signup and view all the answers

    In photosynthesis, what is the immediate source of hydrogen atoms that are incorporated into organic compounds during the light-independent reactions?

    <p>Splitting of water molecules during the light-dependent reactions. (C)</p> Signup and view all the answers

    How does carbon dioxide enrichment affect carbon fixation during photosynthesis?

    <p>It allows more carbon atoms to be combined into organic compounds. (A)</p> Signup and view all the answers

    Why is the splitting of water (photolysis) essential during the light-dependent reactions of photosynthesis?

    <p>It provides electrons to replace those lost by chlorophyll and generates hydrogen ions for ATP synthesis. (A)</p> Signup and view all the answers

    Exposure to which of the following types of radiation is LEAST likely to significantly increase the rate of mutation in DNA?

    <p>Infrared radiation (D)</p> Signup and view all the answers

    Which of the following best describes how viruses can induce mutations?

    <p>By incorporating their genetic material into the host cell's genome, disrupting normal gene function. (A)</p> Signup and view all the answers

    A point mutation occurs in a gene sequence, resulting in a codon change from AGU to AGC. Both codons code for serine. What type of mutation is this an example of?

    <p>Silent mutation (D)</p> Signup and view all the answers

    Which of the following is the MOST direct consequence of a frameshift mutation in a protein-coding gene?

    <p>An altered reading frame leading to a significantly different amino acid sequence from the point of mutation onward. (C)</p> Signup and view all the answers

    If a cell with a mutated BRCA1 gene is exposed to a mutagen, what is the MOST likely outcome?

    <p>The cell will have a reduced capacity to repair DNA damage, potentially leading to further mutations and increased cancer risk. (B)</p> Signup and view all the answers

    A researcher is studying a new chemical compound and wants to determine if it is a mutagen. Which of the following experimental designs would be the MOST appropriate first step?

    <p>Expose the compound to cells and observe any changes in DNA sequence. (D)</p> Signup and view all the answers

    If a DNA sequence originally reads 5'-GCT-3' and, after a mutation, reads 5'-GCTT-3', what type of mutation has occurred?

    <p>A frameshift mutation due to insertion (C)</p> Signup and view all the answers

    How does the malfunction of BRCA1 contribute to an increased risk of cancer?

    <p>By impairing the repair of DNA damage, leading to accumulation of mutations. (D)</p> Signup and view all the answers

    Which of the following is the MOST direct outcome predicted by CO2 enrichment experiments?

    <p>The effects of altered CO2 levels on plant growth and photosynthesis rates. (B)</p> Signup and view all the answers

    What is the PRIMARY difference between enclosed greenhouse experiments and free air CO2 enrichment (FACE) experiments?

    <p>Greenhouse experiments allow for greater control of variables, while FACE experiments involve natural conditions. (D)</p> Signup and view all the answers

    A scientist observes that a plant's photosynthetic rate plateaus despite increasing light intensity. According to the concept of limiting factors, which of the following is the MOST likely explanation?

    <p>Another factor, such as carbon dioxide levels or temperature, is limiting the reaction rate. (D)</p> Signup and view all the answers

    A researcher wants to indirectly measure the rate of carbon dioxide uptake by a plant in an experiment. Which of the following methods would be MOST appropriate?

    <p>Measuring the dry weight of the plant over time. (C)</p> Signup and view all the answers

    Why is a control group necessary when measuring oxygen production in a plant photosynthesis experiment?

    <p>To establish the basal levels of cell respiration in the plant. (A)</p> Signup and view all the answers

    Which statement BEST describes how energy is stored within organic molecules?

    <p>Energy is stored as chemical energy in the covalent bonds (as high-energy electrons). (B)</p> Signup and view all the answers

    What structural feature of ATP makes it readily usable for energy transfer within cells?

    <p>The three phosphate groups, especially the terminal phosphate. (C)</p> Signup and view all the answers

    How do coenzymes facilitate enzymatic reactions?

    <p>By shuttling components needed for the reaction. (B)</p> Signup and view all the answers

    What is the IMMEDIATE effect of hydrolyzing ATP to ADP and phosphate?

    <p>It releases a relatively small amount of energy. (C)</p> Signup and view all the answers

    When ATP detaches from a protein pump, what is the DIRECT consequence?

    <p>Energy is released, causing a change in the molecule. (B)</p> Signup and view all the answers

    A mutation in a somatic cell of a multicellular organism has occurred. Which of the following is the MOST likely outcome?

    <p>The mutation may contribute to the development of cancer in the organism. (C)</p> Signup and view all the answers

    Why is genetic diversity important for a species' survival in a changing environment?

    <p>It provides the raw material for natural selection to act upon, allowing the population to evolve and adapt. (C)</p> Signup and view all the answers

    Which of the following statements accurately describes the relationship between gametes, zygotes, and meiosis in a sexual life cycle?

    <p>Gametes are haploid cells formed through meiosis, and they fuse to form diploid zygotes. (D)</p> Signup and view all the answers

    Consider a plant with the genotype AaBb, where A and B are dominant alleles. What is the term used to describe the possible combinations of alleles this plant inherited?

    <p>Genotype (D)</p> Signup and view all the answers

    In genetics, what distinguishes a heterozygous genotype from a homozygous genotype?

    <p>A heterozygous genotype has two different alleles, while a homozygous genotype has two identical alleles. (A)</p> Signup and view all the answers

    Which of the following BEST illustrates the concept of phenotype?

    <p>The observable characteristics of an organism, such as height or hair color. (C)</p> Signup and view all the answers

    What term BEST describes the phenomenon where freshwater snails develop smaller shells in the presence of predators?

    <p>Phenotypic plasticity (D)</p> Signup and view all the answers

    How does phenotypic plasticity differ from a change in genotype?

    <p>Phenotypic plasticity involves changes in gene expression without altering the DNA sequence, while a change in genotype involves an alteration of the DNA sequence. (D)</p> Signup and view all the answers

    In genetic crosses with plants, what is the purpose of transferring pollen from the anthers to the stigmas?

    <p>To facilitate fertilization and control breeding between specific plants. (B)</p> Signup and view all the answers

    In the context of genetic crosses, what do the terms P generation, F1 generation, and F2 generation represent?

    <p>Parental generation, first filial generation (offspring), and second filial generation, respectively. (A)</p> Signup and view all the answers

    Flashcards

    Enzyme Structure Change

    Changes in enzyme structure enhance catalytic activity by stressing substrate bonds and lowering activation energy.

    Factors Affecting Enzyme Activity

    Enzyme activity can be influenced by temperature, pH, substrate concentration, and enzyme concentration.

    Molecular Collisions

    Enzymes and substrates must collide correctly in orientation for a reaction to occur at the active site.

    Denaturation

    Denaturation unfolds proteins, disrupting the active site and diminishing enzyme activity.

    Signup and view all the flashcards

    Optimum Temperature

    Each enzyme has a specific temperature at which its activity peaks before denaturation occurs.

    Signup and view all the flashcards

    Effect of pH on Enzymes

    Changes in pH alter an enzyme's charge, solubility, and shape, impacting reaction rates.

    Signup and view all the flashcards

    Substrate Concentration Effect

    Increasing substrate concentration raises enzyme activity until saturation occurs, where all active sites are occupied.

    Signup and view all the flashcards

    Photosynthesis Process

    Photosynthesis converts solar energy into chemical energy in organic compounds for plants.

    Signup and view all the flashcards

    Photosynthesizing Pigment

    Chlorophyll is the main pigment that absorbs red and blue light for photosynthesis.

    Signup and view all the flashcards

    Light Spectrum in Photosynthesis

    Photosynthesis utilizes visible light wavelengths (400-700nm) for energy absorption, seen as colors.

    Signup and view all the flashcards

    Action Spectrum

    Wavelengths used by pigments in photosynthesis.

    Signup and view all the flashcards

    Absorption Spectrum

    Wavelengths absorbed by photosynthetic pigments.

    Signup and view all the flashcards

    Chromatography

    Technique used to separate pigments by size.

    Signup and view all the flashcards

    Light-Dependent Reactions

    Convert light energy into ATP.

    Signup and view all the flashcards

    Photolysis

    Splitting of water molecules during photosynthesis.

    Signup and view all the flashcards

    Light-Independent Reactions

    Use ATP to synthesize organic compounds.

    Signup and view all the flashcards

    Retardation Factor

    Value used to identify separated pigments.

    Signup and view all the flashcards

    ATP Synthesis

    Conversion of ADP + Pi into ATP via light energy.

    Signup and view all the flashcards

    Law of Limiting Factors

    The reaction rate is constrained by the most limiting condition.

    Signup and view all the flashcards

    Cell Respiration Factors

    Conditions such as temperature, pH, glucose, and oxygen affect respiration rates.

    Signup and view all the flashcards

    Central Dogma

    The flow of genetic information from DNA to RNA to protein.

    Signup and view all the flashcards

    Transcription

    The process of synthesizing RNA from a DNA template.

    Signup and view all the flashcards

    Gene

    A DNA sequence that is transcribed into RNA.

    Signup and view all the flashcards

    RNA Polymerase

    The enzyme that binds to DNA and synthesizes RNA during transcription.

    Signup and view all the flashcards

    Sense Strand

    The DNA strand that has the base sequence copied into RNA.

    Signup and view all the flashcards

    Complementary Base Pairing

    Bases pair through hydrogen bonds, ensuring correct RNA formation.

    Signup and view all the flashcards

    Fossil Fuels

    Natural substances used to produce energy, releasing CO2.

    Signup and view all the flashcards

    Photosynthesis

    Process by which plants convert CO2 and sunlight into glucose.

    Signup and view all the flashcards

    Limiting Factors

    Conditions that limit the rate of photosynthesis.

    Signup and view all the flashcards

    CO2 Enrichment

    Experiments that increase CO2 to study its effects on plants.

    Signup and view all the flashcards

    Light Intensity

    The amount of light energy hitting a surface, affecting photosynthesis.

    Signup and view all the flashcards

    ATP - Adenosine Triphosphate

    Molecule storing and transferring energy within cells.

    Signup and view all the flashcards

    Oxygen Production

    The release of O2 during photosynthesis, measurable via gas volume.

    Signup and view all the flashcards

    Coenzymes

    Organic molecules that assist enzymes in catalyzing reactions.

    Signup and view all the flashcards

    Cell Respiration

    Process by which cells convert glucose and oxygen into energy.

    Signup and view all the flashcards

    Enclosed Greenhouse Experiments

    Controlled settings to study plant growth and CO2 effects.

    Signup and view all the flashcards

    Somatic Mutations

    Mutations occurring in body cells, not inherited.

    Signup and view all the flashcards

    Germ-line Mutations

    Mutations in gametes that can be inherited.

    Signup and view all the flashcards

    Genetic Variation

    Diversity in gene alleles within a population.

    Signup and view all the flashcards

    Haploid

    Cells with one copy of each chromosome.

    Signup and view all the flashcards

    Diploid

    Cells with two copies of each chromosome.

    Signup and view all the flashcards

    Genotype

    Combination of alleles inherited by an organism.

    Signup and view all the flashcards

    Phenotype

    Observable characteristics of an organism.

    Signup and view all the flashcards

    Polymorphism

    Multiple distinct phenotypes existing for a trait.

    Signup and view all the flashcards

    Phenotypic Plasticity

    Alteration of traits by environmental conditions.

    Signup and view all the flashcards

    Punnett Squares

    Tool to represent genetic crosses visually.

    Signup and view all the flashcards

    Proofreading Errors

    Mistakes during DNA replication that can lead to mutations.

    Signup and view all the flashcards

    Mutagens

    Agents that induce permanent changes in genetic material.

    Signup and view all the flashcards

    Point Mutations

    Modification of a single nucleotide in DNA sequence.

    Signup and view all the flashcards

    Base Substitutions

    Replacing one base in DNA with another.

    Signup and view all the flashcards

    Silent Mutation

    Base substitution that does not change the amino acid produced.

    Signup and view all the flashcards

    Frameshift Mutations

    Insertions or deletions that shift the reading frame of DNA.

    Signup and view all the flashcards

    BRCA1 Gene

    A tumor suppressor gene that repairs DNA and prevents mutations.

    Signup and view all the flashcards

    Nonsense Mutation

    Base substitution that creates a STOP codon, terminating protein synthesis early.

    Signup and view all the flashcards

    Study Notes

    Metabolism

    • Metabolism is the totality of chemical reactions within a cell or organism
    • Metabolic reactions have two key functions
      • They provide energy for life processes (e.g., movement, reproduction)
      • They enable the synthesis and assimilation of new organic materials
    • Nutrients are converted into new materials and energy (waste is excreted)
    • Metabolic reactions can be anabolic or catabolic
      • Anabolism: Building complex macromolecules from simpler biomolecules; requires energy
      • Catabolism: Breaking down macromolecules into simpler molecules; releases energy

    Enzymes

    • Enzymes are globular proteins that act as biological catalysts, increasing reaction rates
    • Enzymes are not consumed by the reaction and can be reused
    • Enzyme names often end in "-ase" (e.g., lipase)
    • Enzymes catalyze the conversion of reactants (substrates) into products
    • The active site of an enzyme is a region on its surface that is complementary in shape and charge to the substrate
    • The active site is specific to the substrate
    • Enzymes catalyze reactions by lowering the activation energy, threshold (EA)
    • Activation energy is the energy required to start a reaction

    Enzyme Activity

    • Enzyme activity can be measured by the consumption of substrates or formation of products
    • Methods to measure include:
      • Measuring pressure change (e.g. catalase)
      • Percentage weight loss (e.g. pectinase)
      • Color change (e.g. pepsin)
      • Mass of precipitate formed (e.g., rennin)
    • Factors affecting enzyme activity include:
      • Temperature
      • pH
      • Substrate concentration
      • Enzyme concentration

    Catalysis

    • Enzyme's active site is not rigid and changes shape to fit the substrate (induced fit model)
    • This change in structure promotes catalytic activity
    • The conformational changes stress bonds in the substrate and increase reactivity (lower activation energy)

    Molecular Collisions

    • Enzymes and substrates must collide in the correct orientation to interact
    • Increasing kinetic energy and particle concentration increases successful collisions
    • Enzymes or substrates can be immobilized (e.g., embedded in a membrane)

    Denaturation

    • Enzyme activity depends on the 3D shape of the protein (tertiary structure)
    • Denaturation breaks the bonds involved in protein folding

    Temperature

    • Low temperatures limit molecular motion and activation energy
    • Increasing temperature increases kinetic energy and the frequency of collisions
      • Peak reaction rate at optimal temperature, dependent on the enzyme
    • High temperatures decrease activity due to denaturation

    pH

    • Changing pH alters enzyme charge, affecting both solubility and shape
    • Enzymes have an optimal pH at which activity is highest
    • Activity decreases outside this optimal range (denaturation)

    Substrate Concentration

    • Increasing substrate concentration increases enzyme activity, only up to a point
    • At high substrate concentrations, the solution becomes saturated, and all active sites are occupied

    Photosynthesis

    • Photosynthesis is a process where organisms use light energy to synthesize organic compounds
    • Photosynthesis equation: CO2 + H2O → C6H12O6 + O2

    Light Spectrum

    • Photosynthesis uses wavelengths (400-700 nm) within the visible spectrum
    • Colors: red, orange, yellow, green, blue, indigo, violet (longest to shortest wavelength)

    Photosynthetic Pigments

    • Chlorophyll is the primary photosynthetic pigment
    • Chlorophyll absorbs red and blue light, reflecting green light
    • Absorption spectra shows wavelengths absorbed by pigments
    • Action spectra shows wavelengths used in photosynthesis
    • Chromatography is a technique to separate pigments by size

    Photosynthetic Stages

    • Light-dependent reactions convert light energy into chemical energy (ATP)
    • Light-independent reactions use this energy to synthesize organic compounds (e.g., glucose)

    Carbon Dioxide Enrichment

    • CO2 is a substrate in photosynthesis
    • Increasing atmospheric CO2 can affect global temperatures and rates of photosynthesis
    • Experiments (e.g., FACE) can predict outcomes of CO2 level changes

    Limiting Factors of Photosynthesis

    • Factors that limit rates: light intensity, wavelengths of light, CO2 levels, temperature, pH
    • Rates are limited by the factor nearest its minimum value

    Photosynthesis Experiments

    • Experiments measure uptake of inputs (e.g., CO2) or outputs (e.g., O2)

    Cell Respiration

    • Cell respiration is the controlled release of energy from organic compounds (e.g., glucose)
      • This process is primarily used to produce ATP
    • Respiration can be either anaerobic or aerobic

    ATP

    • ATP (adenosine triphosphate) is a molecule that stores chemical energy in its covalent bonds
    • It is immediately available as an energy source for cells

    Types of Respiration

    • Anaerobic respiration partially breaks down organic compounds (e.g., glucose) producing small amounts of ATP and lactate in animals
    • Aerobic respiration completely breaks down organic compounds in the presence of oxygen into carbon dioxide and water, producing a larger amount of ATP

    Respiration Comparison

    • Aerobic respiration produces more ATP than anaerobic
    • Aerobic respiration requires oxygen and occurs in mitochondria
    • Anaerobic respiration occurs in the cytoplasm without oxygen (sometimes in muscles)

    Limiting Factors of Cell Respiration

    • Cell respiration is dependent on temperature, pH, glucose concentration, oxygen levels
    • Factors near their minimum affect its rate

    Transcription and Translation

    • The fundamental process of converting genetic information into protein
    • Transcription produces RNA from a DNA template (gene)
    • Translation produces proteins from an RNA template (mRNA)
    • RNA polymerase plays a crucial role in transcription

    Mutations

    • A gene mutation is a change in the nucleotide structure of a gene
      • Substitution: One base is replaced with another
        • Silent: No change in amino acid
        • Missense: Changes one amino acid
        • Nonsense: Creates a stop codon
      • Insertion/Deletion (frameshift): Adding or removing a base(s), altering the reading frame of codons

    Causes of Mutations

    • Proofreading errors during DNA replication
    • Mutagens (physical, chemical, biological agents) increase mutation rates

    DNA Replication

    • DNA replication is the process of copying DNA during cell reproduction
    • The semi-conservative model describes one old strand and one new strand in each resulting DNA molecule

    DNA Technologies

    • PCR (polymerase chain reaction) is used to amplify DNA
    • Gel electrophoresis separates DNA fragments based on size

    Gene Cloning

    • Gene cloning introduces a gene into a different organism by using a vector(i.e. plasmid)

    Sequencing

    • Sanger method is used to determine base sequence in DNA
    • The modified PCR process is used to produce DNA fragments with specific lengths

    Polygenic Inheritance

    • Polygenic traits are influenced by multiple genes and exhibit continuous variation
    • Examples include skin color, height

    Sex Linkage

    • Genes located on sex chromosomes often show different inheritance patterns due to sex bias in genotypes and phenotypes

    Pedigree Charts

    • Pedigree charts illustrate inheritance patterns across generations.

    Phenotypic Plasticity/ Genetic Crosses

    • Phenotypic plasticity: how physical characteristics can change due to the environment without changing the genotype
    • Genetic crosses: Experiments to demonstrate inheritance principles (like Mendel's pea plant crosses)

    Punnett Squares

    • Graphic method to predict offspring genotypes and phenotypes from parental crosses

    Modes of Inheritance

    • Different patterns of how alleles interact to determine traits:
      • Complete dominance, incomplete dominance, co-dominance
      • Sex linkage

    Genetic Variation

    • Mutations are the only source of new alleles in a population
    • Sexual reproduction increases genetic variation
    • Genetic variation is crucial for species survival and adaptation

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    5th Part Bio Exam PDF

    Description

    Explore fundamental biological concepts including limiting factors, transcription mechanisms, and enzyme functions. This quiz covers the roles of RNA polymerase, DNA template sequences in RNA synthesis, and enzyme activity measurement. Understanding these basics is crucial for grasping molecular biology.

    More Like This

    RNA Synthesis
    30 questions

    RNA Synthesis

    EffectualJubilation avatar
    EffectualJubilation
    2M03 Chapter 11
    40 questions

    2M03 Chapter 11

    EarnestLake avatar
    EarnestLake
    Transcription of DNA and Types of RNA
    10 questions
    RNA Transcription Quiz
    10 questions

    RNA Transcription Quiz

    PeaceableIslamicArt avatar
    PeaceableIslamicArt
    Use Quizgecko on...
    Browser
    Browser