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Questions and Answers
According to the law of limiting factors, if a reaction depends on multiple conditions, what determines the reaction rate?
According to the law of limiting factors, if a reaction depends on multiple conditions, what determines the reaction rate?
Which of the following cellular processes is NOT directly mentioned as being dependent on specific environmental conditions?
Which of the following cellular processes is NOT directly mentioned as being dependent on specific environmental conditions?
What is the primary function of RNA polymerase during transcription?
What is the primary function of RNA polymerase during transcription?
In the process of transcription, what dictates the sequence of the newly synthesized RNA molecule?
In the process of transcription, what dictates the sequence of the newly synthesized RNA molecule?
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If a template DNA strand has the sequence 3'-TTCAGTC-5'
, what would be the corresponding RNA sequence produced during transcription?
If a template DNA strand has the sequence 3'-TTCAGTC-5'
, what would be the corresponding RNA sequence produced during transcription?
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Which of the following BEST describes the role of the 'sense strand' (or 'coding strand') in transcription?
Which of the following BEST describes the role of the 'sense strand' (or 'coding strand') in transcription?
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What event signals the termination of transcription?
What event signals the termination of transcription?
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What is the relationship between a gene and an RNA transcript?
What is the relationship between a gene and an RNA transcript?
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How does a conformational change in an enzyme promote catalytic activity?
How does a conformational change in an enzyme promote catalytic activity?
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Which of the following methods would NOT effectively measure enzyme activity?
Which of the following methods would NOT effectively measure enzyme activity?
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An enzyme-catalyzed reaction initially proceeds slowly. Which factor change is most likely to increase the reaction rate?
An enzyme-catalyzed reaction initially proceeds slowly. Which factor change is most likely to increase the reaction rate?
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How does increasing the level of molecular motion affect enzyme activity?
How does increasing the level of molecular motion affect enzyme activity?
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What is the direct effect of enzyme denaturation on enzyme activity?
What is the direct effect of enzyme denaturation on enzyme activity?
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How does changing the pH affect enzyme activity?
How does changing the pH affect enzyme activity?
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At a certain substrate concentration, the rate of an enzyme-catalyzed reaction reaches a plateau. Why does this occur?
At a certain substrate concentration, the rate of an enzyme-catalyzed reaction reaches a plateau. Why does this occur?
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In photosynthesis, what is the role of light energy?
In photosynthesis, what is the role of light energy?
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How do absorption spectra and action spectra differ in the context of photosynthesis?
How do absorption spectra and action spectra differ in the context of photosynthesis?
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In chromatography, what determines the separation of photosynthetic pigments?
In chromatography, what determines the separation of photosynthetic pigments?
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Which of the following wavelength ranges corresponds to visible light used in photosynthesis?
Which of the following wavelength ranges corresponds to visible light used in photosynthesis?
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What is the primary role of light-dependent reactions in photosynthesis?
What is the primary role of light-dependent reactions in photosynthesis?
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Why do plants appear green?
Why do plants appear green?
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How do light-dependent reactions contribute to the subsequent light-independent reactions?
How do light-dependent reactions contribute to the subsequent light-independent reactions?
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What is the role of ATP in the light-independent reactions? Choose the best answer.
What is the role of ATP in the light-independent reactions? Choose the best answer.
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In photosynthesis, what is the immediate source of hydrogen atoms that are incorporated into organic compounds during the light-independent reactions?
In photosynthesis, what is the immediate source of hydrogen atoms that are incorporated into organic compounds during the light-independent reactions?
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How does carbon dioxide enrichment affect carbon fixation during photosynthesis?
How does carbon dioxide enrichment affect carbon fixation during photosynthesis?
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Why is the splitting of water (photolysis) essential during the light-dependent reactions of photosynthesis?
Why is the splitting of water (photolysis) essential during the light-dependent reactions of photosynthesis?
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Exposure to which of the following types of radiation is LEAST likely to significantly increase the rate of mutation in DNA?
Exposure to which of the following types of radiation is LEAST likely to significantly increase the rate of mutation in DNA?
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Which of the following best describes how viruses can induce mutations?
Which of the following best describes how viruses can induce mutations?
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A point mutation occurs in a gene sequence, resulting in a codon change from AGU to AGC. Both codons code for serine. What type of mutation is this an example of?
A point mutation occurs in a gene sequence, resulting in a codon change from AGU to AGC. Both codons code for serine. What type of mutation is this an example of?
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Which of the following is the MOST direct consequence of a frameshift mutation in a protein-coding gene?
Which of the following is the MOST direct consequence of a frameshift mutation in a protein-coding gene?
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If a cell with a mutated BRCA1 gene is exposed to a mutagen, what is the MOST likely outcome?
If a cell with a mutated BRCA1 gene is exposed to a mutagen, what is the MOST likely outcome?
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A researcher is studying a new chemical compound and wants to determine if it is a mutagen. Which of the following experimental designs would be the MOST appropriate first step?
A researcher is studying a new chemical compound and wants to determine if it is a mutagen. Which of the following experimental designs would be the MOST appropriate first step?
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If a DNA sequence originally reads 5'-GCT-3' and, after a mutation, reads 5'-GCTT-3', what type of mutation has occurred?
If a DNA sequence originally reads 5'-GCT-3' and, after a mutation, reads 5'-GCTT-3', what type of mutation has occurred?
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How does the malfunction of BRCA1 contribute to an increased risk of cancer?
How does the malfunction of BRCA1 contribute to an increased risk of cancer?
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Which of the following is the MOST direct outcome predicted by CO2 enrichment experiments?
Which of the following is the MOST direct outcome predicted by CO2 enrichment experiments?
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What is the PRIMARY difference between enclosed greenhouse experiments and free air CO2 enrichment (FACE) experiments?
What is the PRIMARY difference between enclosed greenhouse experiments and free air CO2 enrichment (FACE) experiments?
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A scientist observes that a plant's photosynthetic rate plateaus despite increasing light intensity. According to the concept of limiting factors, which of the following is the MOST likely explanation?
A scientist observes that a plant's photosynthetic rate plateaus despite increasing light intensity. According to the concept of limiting factors, which of the following is the MOST likely explanation?
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A researcher wants to indirectly measure the rate of carbon dioxide uptake by a plant in an experiment. Which of the following methods would be MOST appropriate?
A researcher wants to indirectly measure the rate of carbon dioxide uptake by a plant in an experiment. Which of the following methods would be MOST appropriate?
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Why is a control group necessary when measuring oxygen production in a plant photosynthesis experiment?
Why is a control group necessary when measuring oxygen production in a plant photosynthesis experiment?
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Which statement BEST describes how energy is stored within organic molecules?
Which statement BEST describes how energy is stored within organic molecules?
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What structural feature of ATP makes it readily usable for energy transfer within cells?
What structural feature of ATP makes it readily usable for energy transfer within cells?
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How do coenzymes facilitate enzymatic reactions?
How do coenzymes facilitate enzymatic reactions?
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What is the IMMEDIATE effect of hydrolyzing ATP to ADP and phosphate?
What is the IMMEDIATE effect of hydrolyzing ATP to ADP and phosphate?
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When ATP detaches from a protein pump, what is the DIRECT consequence?
When ATP detaches from a protein pump, what is the DIRECT consequence?
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A mutation in a somatic cell of a multicellular organism has occurred. Which of the following is the MOST likely outcome?
A mutation in a somatic cell of a multicellular organism has occurred. Which of the following is the MOST likely outcome?
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Why is genetic diversity important for a species' survival in a changing environment?
Why is genetic diversity important for a species' survival in a changing environment?
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Which of the following statements accurately describes the relationship between gametes, zygotes, and meiosis in a sexual life cycle?
Which of the following statements accurately describes the relationship between gametes, zygotes, and meiosis in a sexual life cycle?
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Consider a plant with the genotype AaBb, where A and B are dominant alleles. What is the term used to describe the possible combinations of alleles this plant inherited?
Consider a plant with the genotype AaBb, where A and B are dominant alleles. What is the term used to describe the possible combinations of alleles this plant inherited?
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In genetics, what distinguishes a heterozygous genotype from a homozygous genotype?
In genetics, what distinguishes a heterozygous genotype from a homozygous genotype?
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Which of the following BEST illustrates the concept of phenotype?
Which of the following BEST illustrates the concept of phenotype?
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What term BEST describes the phenomenon where freshwater snails develop smaller shells in the presence of predators?
What term BEST describes the phenomenon where freshwater snails develop smaller shells in the presence of predators?
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How does phenotypic plasticity differ from a change in genotype?
How does phenotypic plasticity differ from a change in genotype?
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In genetic crosses with plants, what is the purpose of transferring pollen from the anthers to the stigmas?
In genetic crosses with plants, what is the purpose of transferring pollen from the anthers to the stigmas?
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In the context of genetic crosses, what do the terms P generation, F1 generation, and F2 generation represent?
In the context of genetic crosses, what do the terms P generation, F1 generation, and F2 generation represent?
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Flashcards
Enzyme Structure Change
Enzyme Structure Change
Changes in enzyme structure enhance catalytic activity by stressing substrate bonds and lowering activation energy.
Factors Affecting Enzyme Activity
Factors Affecting Enzyme Activity
Enzyme activity can be influenced by temperature, pH, substrate concentration, and enzyme concentration.
Molecular Collisions
Molecular Collisions
Enzymes and substrates must collide correctly in orientation for a reaction to occur at the active site.
Denaturation
Denaturation
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Optimum Temperature
Optimum Temperature
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Effect of pH on Enzymes
Effect of pH on Enzymes
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Substrate Concentration Effect
Substrate Concentration Effect
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Photosynthesis Process
Photosynthesis Process
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Photosynthesizing Pigment
Photosynthesizing Pigment
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Light Spectrum in Photosynthesis
Light Spectrum in Photosynthesis
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Action Spectrum
Action Spectrum
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Absorption Spectrum
Absorption Spectrum
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Chromatography
Chromatography
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Light-Dependent Reactions
Light-Dependent Reactions
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Photolysis
Photolysis
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Light-Independent Reactions
Light-Independent Reactions
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Retardation Factor
Retardation Factor
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ATP Synthesis
ATP Synthesis
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Law of Limiting Factors
Law of Limiting Factors
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Cell Respiration Factors
Cell Respiration Factors
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Central Dogma
Central Dogma
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Transcription
Transcription
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Gene
Gene
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RNA Polymerase
RNA Polymerase
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Sense Strand
Sense Strand
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Complementary Base Pairing
Complementary Base Pairing
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Fossil Fuels
Fossil Fuels
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Photosynthesis
Photosynthesis
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Limiting Factors
Limiting Factors
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CO2 Enrichment
CO2 Enrichment
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Light Intensity
Light Intensity
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ATP - Adenosine Triphosphate
ATP - Adenosine Triphosphate
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Oxygen Production
Oxygen Production
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Coenzymes
Coenzymes
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Cell Respiration
Cell Respiration
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Enclosed Greenhouse Experiments
Enclosed Greenhouse Experiments
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Somatic Mutations
Somatic Mutations
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Germ-line Mutations
Germ-line Mutations
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Genetic Variation
Genetic Variation
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Haploid
Haploid
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Diploid
Diploid
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Genotype
Genotype
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Phenotype
Phenotype
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Polymorphism
Polymorphism
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Phenotypic Plasticity
Phenotypic Plasticity
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Punnett Squares
Punnett Squares
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Proofreading Errors
Proofreading Errors
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Mutagens
Mutagens
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Point Mutations
Point Mutations
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Base Substitutions
Base Substitutions
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Silent Mutation
Silent Mutation
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Frameshift Mutations
Frameshift Mutations
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BRCA1 Gene
BRCA1 Gene
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Nonsense Mutation
Nonsense Mutation
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Study Notes
Metabolism
- Metabolism is the totality of chemical reactions within a cell or organism
- Metabolic reactions have two key functions
- They provide energy for life processes (e.g., movement, reproduction)
- They enable the synthesis and assimilation of new organic materials
- Nutrients are converted into new materials and energy (waste is excreted)
- Metabolic reactions can be anabolic or catabolic
- Anabolism: Building complex macromolecules from simpler biomolecules; requires energy
- Catabolism: Breaking down macromolecules into simpler molecules; releases energy
Enzymes
- Enzymes are globular proteins that act as biological catalysts, increasing reaction rates
- Enzymes are not consumed by the reaction and can be reused
- Enzyme names often end in "-ase" (e.g., lipase)
- Enzymes catalyze the conversion of reactants (substrates) into products
- The active site of an enzyme is a region on its surface that is complementary in shape and charge to the substrate
- The active site is specific to the substrate
- Enzymes catalyze reactions by lowering the activation energy, threshold (EA)
- Activation energy is the energy required to start a reaction
Enzyme Activity
- Enzyme activity can be measured by the consumption of substrates or formation of products
- Methods to measure include:
- Measuring pressure change (e.g. catalase)
- Percentage weight loss (e.g. pectinase)
- Color change (e.g. pepsin)
- Mass of precipitate formed (e.g., rennin)
- Factors affecting enzyme activity include:
- Temperature
- pH
- Substrate concentration
- Enzyme concentration
Catalysis
- Enzyme's active site is not rigid and changes shape to fit the substrate (induced fit model)
- This change in structure promotes catalytic activity
- The conformational changes stress bonds in the substrate and increase reactivity (lower activation energy)
Molecular Collisions
- Enzymes and substrates must collide in the correct orientation to interact
- Increasing kinetic energy and particle concentration increases successful collisions
- Enzymes or substrates can be immobilized (e.g., embedded in a membrane)
Denaturation
- Enzyme activity depends on the 3D shape of the protein (tertiary structure)
- Denaturation breaks the bonds involved in protein folding
Temperature
- Low temperatures limit molecular motion and activation energy
- Increasing temperature increases kinetic energy and the frequency of collisions
- Peak reaction rate at optimal temperature, dependent on the enzyme
- High temperatures decrease activity due to denaturation
pH
- Changing pH alters enzyme charge, affecting both solubility and shape
- Enzymes have an optimal pH at which activity is highest
- Activity decreases outside this optimal range (denaturation)
Substrate Concentration
- Increasing substrate concentration increases enzyme activity, only up to a point
- At high substrate concentrations, the solution becomes saturated, and all active sites are occupied
Photosynthesis
- Photosynthesis is a process where organisms use light energy to synthesize organic compounds
- Photosynthesis equation: CO2 + H2O → C6H12O6 + O2
Light Spectrum
- Photosynthesis uses wavelengths (400-700 nm) within the visible spectrum
- Colors: red, orange, yellow, green, blue, indigo, violet (longest to shortest wavelength)
Photosynthetic Pigments
- Chlorophyll is the primary photosynthetic pigment
- Chlorophyll absorbs red and blue light, reflecting green light
- Absorption spectra shows wavelengths absorbed by pigments
- Action spectra shows wavelengths used in photosynthesis
- Chromatography is a technique to separate pigments by size
Photosynthetic Stages
- Light-dependent reactions convert light energy into chemical energy (ATP)
- Light-independent reactions use this energy to synthesize organic compounds (e.g., glucose)
Carbon Dioxide Enrichment
- CO2 is a substrate in photosynthesis
- Increasing atmospheric CO2 can affect global temperatures and rates of photosynthesis
- Experiments (e.g., FACE) can predict outcomes of CO2 level changes
Limiting Factors of Photosynthesis
- Factors that limit rates: light intensity, wavelengths of light, CO2 levels, temperature, pH
- Rates are limited by the factor nearest its minimum value
Photosynthesis Experiments
- Experiments measure uptake of inputs (e.g., CO2) or outputs (e.g., O2)
Cell Respiration
- Cell respiration is the controlled release of energy from organic compounds (e.g., glucose)
- This process is primarily used to produce ATP
- Respiration can be either anaerobic or aerobic
ATP
- ATP (adenosine triphosphate) is a molecule that stores chemical energy in its covalent bonds
- It is immediately available as an energy source for cells
Types of Respiration
- Anaerobic respiration partially breaks down organic compounds (e.g., glucose) producing small amounts of ATP and lactate in animals
- Aerobic respiration completely breaks down organic compounds in the presence of oxygen into carbon dioxide and water, producing a larger amount of ATP
Respiration Comparison
- Aerobic respiration produces more ATP than anaerobic
- Aerobic respiration requires oxygen and occurs in mitochondria
- Anaerobic respiration occurs in the cytoplasm without oxygen (sometimes in muscles)
Limiting Factors of Cell Respiration
- Cell respiration is dependent on temperature, pH, glucose concentration, oxygen levels
- Factors near their minimum affect its rate
Transcription and Translation
- The fundamental process of converting genetic information into protein
- Transcription produces RNA from a DNA template (gene)
- Translation produces proteins from an RNA template (mRNA)
- RNA polymerase plays a crucial role in transcription
Mutations
- A gene mutation is a change in the nucleotide structure of a gene
- Substitution: One base is replaced with another
- Silent: No change in amino acid
- Missense: Changes one amino acid
- Nonsense: Creates a stop codon
- Insertion/Deletion (frameshift): Adding or removing a base(s), altering the reading frame of codons
- Substitution: One base is replaced with another
Causes of Mutations
- Proofreading errors during DNA replication
- Mutagens (physical, chemical, biological agents) increase mutation rates
DNA Replication
- DNA replication is the process of copying DNA during cell reproduction
- The semi-conservative model describes one old strand and one new strand in each resulting DNA molecule
DNA Technologies
- PCR (polymerase chain reaction) is used to amplify DNA
- Gel electrophoresis separates DNA fragments based on size
Gene Cloning
- Gene cloning introduces a gene into a different organism by using a vector(i.e. plasmid)
Sequencing
- Sanger method is used to determine base sequence in DNA
- The modified PCR process is used to produce DNA fragments with specific lengths
Polygenic Inheritance
- Polygenic traits are influenced by multiple genes and exhibit continuous variation
- Examples include skin color, height
Sex Linkage
- Genes located on sex chromosomes often show different inheritance patterns due to sex bias in genotypes and phenotypes
Pedigree Charts
- Pedigree charts illustrate inheritance patterns across generations.
Phenotypic Plasticity/ Genetic Crosses
- Phenotypic plasticity: how physical characteristics can change due to the environment without changing the genotype
- Genetic crosses: Experiments to demonstrate inheritance principles (like Mendel's pea plant crosses)
Punnett Squares
- Graphic method to predict offspring genotypes and phenotypes from parental crosses
Modes of Inheritance
- Different patterns of how alleles interact to determine traits:
- Complete dominance, incomplete dominance, co-dominance
- Sex linkage
Genetic Variation
- Mutations are the only source of new alleles in a population
- Sexual reproduction increases genetic variation
- Genetic variation is crucial for species survival and adaptation
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Description
Explore fundamental biological concepts including limiting factors, transcription mechanisms, and enzyme functions. This quiz covers the roles of RNA polymerase, DNA template sequences in RNA synthesis, and enzyme activity measurement. Understanding these basics is crucial for grasping molecular biology.