Transcription and Translation

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Questions and Answers

During transcription, what is the primary role of the template strand?

  • To signal the start of a gene for transcription.
  • To add a guanine with methyl group to the 5' end of transcript.
  • To serve as the strand from which RNA polymerase builds mRNA. (correct)
  • To stop RNA polymerase from transcription.

Which of the following best describes the function of the promoter during transcription?

  • It binds to the mRNA and tells amino acid sequence.
  • It is the strand on which RNA polymerase builds mRNA.
  • It terminates the process of transcription by signaling RNA polymerase to detach.
  • It signals the start of a gene, initiating the transcription process. (correct)

What is the main function of RNA polymerase in transcription?

  • To remove introns and link exons in the mRNA transcript.
  • To add a poly-adenine tail to the 3' end of the mRNA transcript.
  • To bind to the promoter, unwind the DNA helix, and build mRNA. (correct)
  • To signal the end of transcription.

What is the role of the terminator sequence in transcription?

<p>It signals RNA polymerase to stop transcription. (D)</p> Signup and view all the answers

Which of the following modifications must occur to mRNA in a eukaryotic cell before it can be translated?

<p>Addition of a 5' methyl group, addition of a poly-A tail, and removal of introns. (A)</p> Signup and view all the answers

What is the function of mRNA in translation?

<p>Binds to a small ribosomal subunit to initiate translation. (A)</p> Signup and view all the answers

What role does tRNA play during translation?

<p>It binds to mRNA at the start codon and carries amino acids to the ribosome. (C)</p> Signup and view all the answers

Which of the following describes the function of ribosomes in translation?

<p>They read the RNA code and link appropriate amino acids together. (D)</p> Signup and view all the answers

What is the start codon's function in translation?

<p>It signals the start of translation. (B)</p> Signup and view all the answers

What is the function of the stop codon in translation?

<p>Signals to end translation. (C)</p> Signup and view all the answers

Which of the following is a key difference in mRNA processing between eukaryotic and prokaryotic cells?

<p>Eukaryotic mRNA undergoes modifications such as the addition of a 5' cap and a poly-A tail. (B)</p> Signup and view all the answers

How does the addition of a poly-adenine tail to mRNA in eukaryotic cells affect its function?

<p>It protects the mRNA from degradation and enhances translation efficiency. (D)</p> Signup and view all the answers

What would be the most likely effect of a mutation in the promoter region of a gene?

<p>Decreased or absent transcription of the gene. (A)</p> Signup and view all the answers

How do ribosomes identify the correct start codon on the mRNA to initiate translation?

<p>By recognizing a specific sequence of bases surrounding the start codon. (C)</p> Signup and view all the answers

A mutation occurs in a gene such that the stop codon is eliminated. What is the most likely consequence of this mutation?

<p>The ribosome will continue to translate the mRNA, producing an elongated polypeptide. (A)</p> Signup and view all the answers

Which of the following is a direct product of transcription?

<p>A primary RNA transcript that requires processing. (D)</p> Signup and view all the answers

What property of the genetic code allows a single tRNA to recognize multiple codons?

<p>Wobble base pairing. (C)</p> Signup and view all the answers

What is the significance of dehydration synthesis in translation?

<p>The formation of peptide bonds between amino acids. (B)</p> Signup and view all the answers

In what cellular component does translation primarily take place?

<p>The ribosome. (D)</p> Signup and view all the answers

If a cell lacks the enzyme that adds the methyl group to guanine during mRNA processing, what is the most likely consequence?

<p>Reduced translation efficiency and increased mRNA degradation. (A)</p> Signup and view all the answers

Flashcards

Template strand

Strand where RNA polymerase begins building mRNA.

Promoter

Signals the start of gene transcription.

RNA polymerase

Binds to the promoter and builds mRNA.

Terminator

Signals RNA polymerase to stop transcription.

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mRNA Modification 1

Addition of guanine with methyl group to 5' end.

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mRNA Modification 2

mRNA modification with poly-adenine tail.

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mRNA Modification 3

The removal of introns and linking of exons.

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mRNA function

Binds to small ribosomal subunit and tells amino acid sequence.

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tRNA function

Binds to mRNA at the start codon and holds the growing peptide chain.

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Ribosomes function

Reads RNA code and links appropriate amino acids through dehydration synthesis.

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Start codon function

Signals the start of translation.

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Stop codon function

Signals to end translation.

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Study Notes

  • Transcription involves several key components each with a specific function

Template Strand

  • It is the strand that the RNA polymerase uses as a template to create mRNA
  • RNA polymerase starts making mRNA on it

Promoter

  • Signals the start of a gene for transcription

RNA Polymerase

  • It binds to the promoter, unwinding the helix
  • Builds mRNA accordingly

Terminator

  • It's a part of promoter DNA
  • Signals RNA polymerase to stop transcription

mRNA Processing in Eukaryotes

  • The mRNA needs to undergo several modifications before it can be translated in eukaryotic cells
  • A guanine with a methyl group is added to the 5' end of the transcript
  • A poly-adenine tail is added to the 3' end
  • Introns are removed to link exons

mRNA

  • Binds to the small ribosomal subunit and tells amino acid sequence

tRNA

  • Binds to mRNA at the start codon
  • Holds the growing peptide chain
  • Brings amino acids back to the ribosome

Ribosomes

  • Reads the RNA code
  • Links appropriate amino acids together through dehydration synthesis

Start Codon

  • Signals the start of translation

Stop Codon

  • Signals to end translation

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