Traditional Culture in Food Testing Laboratories
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Questions and Answers

What is the focus of the article by Anany et al. (2018)?

  • The viable but non-culturable state in food safety
  • Specific assays for bacteria using adenylate kinase release
  • Detecting foodborne pathogens using phage-based dipstick assay (correct)
  • Efficacy of plant-derived antimicrobials on E. coli virulence
  • What does the article by Bhunia (2014) discuss?

  • Detection speed of foodborne pathogens from one day to one hour (correct)
  • Rapid and ultrasensitive phage-based dipstick assay for foodborne pathogens
  • The viable but non-culturable state in food safety
  • Efficacy of plant-derived antimicrobials on E. coli virulence
  • What is the main topic of the article by Botsaris et al. (2013)?

  • Detecting Mycobacterium avium subspecies paratuberculosis in milk and cheese (correct)
  • Specific assays for bacteria using phage mediated adenylate kinase release
  • A matter of bacterial life and death
  • Efficacy of plant-derived antimicrobials on E. coli virulence
  • Which article emphasizes the importance of the viable but non-culturable state?

    <p>Ayrapetyan &amp; Oliver (2016)</p> Signup and view all the answers

    What aspect does the article by Bogosian & Bourneuf (2001) focus on?

    <p>Understanding bacterial life and death</p> Signup and view all the answers

    Which article discusses controlling E. coli virulence with plant-derived antimicrobials?

    <p>Baskaran et al. (2016)</p> Signup and view all the answers

    What is the main advantage of traditional culture-based methods for food testing?

    <p>They are inexpensive and easy to use</p> Signup and view all the answers

    What is the main limitation of culture-based methods for food testing?

    <p>They require a long time to obtain final confirmation of the species isolated</p> Signup and view all the answers

    What factors can influence the accuracy of culture-based results for food testing?

    <p>The non-uniform distribution and low abundance of pathogens in food samples</p> Signup and view all the answers

    What is the typical timeline for obtaining final confirmation of the species isolated using culture-based methods?

    <p>1 week</p> Signup and view all the answers

    What is a potential limitation of culture-based methods in detecting certain microorganisms in food?

    <p>They are not suitable for detecting injured or VBNC microorganisms</p> Signup and view all the answers

    What are the key steps involved in the culture-based analysis of food samples?

    <p>Selective enrichment, plating on selective media, and biochemical or serological confirmatory tests</p> Signup and view all the answers

    What technique was discussed for detecting viable cells using nucleic acid amplification?

    <p>Preferential detection using novel approaches</p> Signup and view all the answers

    Which of the following statements about the viable but nonculturable (VBNC) state in pathogenic bacteria is true?

    <p>VBNC state allows bacteria to survive in mature apple fruit calyces</p> Signup and view all the answers

    Which technique was used to detect Listeria spp. in the study by Oliveira et al. (2012)?

    <p>Bacteriophage amplification assay with viricidal laser treatment</p> Signup and view all the answers

    Which of the following factors was investigated in the study by Omori et al. (2017) for its influence on the heat resistance of Listeria monocytogenes?

    <p>Lactic acid treatment and post-treatment recovery time</p> Signup and view all the answers

    Which technique was used by Nogva et al. (2003) for DNA-based differentiation of viable and dead bacteria?

    <p>Ethidium monoazide treatment followed by 5'-nuclease PCR</p> Signup and view all the answers

    Which of the following statements about the viable but nonculturable (VBNC) state is false?

    <p>Recent findings on VBNC state in pathogenic bacteria are conclusive</p> Signup and view all the answers

    What does the abbreviation CDC stand for?

    <p>Centers for Disease Control and Prevention</p> Signup and view all the answers

    What is the primary focus of the study by Terio et al. (2017)?

    <p>Investigating the occurrence of hepatitis A, E, and norovirus in ready-to-eat vegetables in Italy</p> Signup and view all the answers

    What is the focus of the study by Trevors (2012)?

    <p>Investigating the expression of genes in dead bacterial cells</p> Signup and view all the answers

    Which of the following is NOT a potential source of foodborne illness mentioned in the text?

    <p>Salmonella</p> Signup and view all the answers

    What is the focus of the study by Wu et al. (2017)?

    <p>Investigating the formation of persister cells in Listeria monocytogenes in response to nisin</p> Signup and view all the answers

    Which of the following is NOT mentioned as a potential source of foodborne illness in the text?

    <p>Hepatitis B virus</p> Signup and view all the answers

    What is the main purpose of the phage-PCR assay mentioned in the text?

    <p>To detect the presence of Mycobacterium avium subsp. paratuberculosis in bulk tank milk</p> Signup and view all the answers

    Which of the following is a key finding from the study on detecting Mycobacterium avium subsp. paratuberculosis in powdered infant formula?

    <p>Viable Mycobacterium avium subsp. paratuberculosis was detected in powdered infant formula using phage-PCR and confirmed by culture</p> Signup and view all the answers

    What is the main focus of the study on rapid on-site phage-mediated detection of generic Escherichia coli in water?

    <p>Using luminescent and visual readout methods</p> Signup and view all the answers

    What is the main purpose of the T7 bacteriophage-conjugated magnetic probe discussed in the text?

    <p>To detect the presence of generic Escherichia coli in water</p> Signup and view all the answers

    What is the main purpose of the information provided on food microbiology in the text?

    <p>To provide background information on the fundamentals and frontiers of food microbiology</p> Signup and view all the answers

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