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Questions and Answers
What is the purpose of performing a total leukocyte count?
What is the purpose of performing a total leukocyte count?
- To determine the number of different types of erythrocytes in one cubic millimeter of blood.
- To count the number of different types of leukocytes in one cubic millimeter of circulating blood. (correct)
- To assess the level of glucose in the blood.
- To measure the pH of the blood.
In a total leukocyte count, the blood sample is diluted 10 times with a fluid that lyses red blood cells.
In a total leukocyte count, the blood sample is diluted 10 times with a fluid that lyses red blood cells.
False (B)
What type of chamber is required for performing a total leukocyte count?
What type of chamber is required for performing a total leukocyte count?
Neubauer chamber
The diluting fluid used in a total leukocyte count contains glacial acetic acid and a drop of ______ to stain the nuclei of WBCs.
The diluting fluid used in a total leukocyte count contains glacial acetic acid and a drop of ______ to stain the nuclei of WBCs.
Match the condition with its effect on leukocyte count:
Match the condition with its effect on leukocyte count:
Which of the following conditions is associated with leukocytosis?
Which of the following conditions is associated with leukocytosis?
Radiotherapy is associated with leukocytosis.
Radiotherapy is associated with leukocytosis.
What is the normal range of leukocyte count in adults (cells/mm³)?
What is the normal range of leukocyte count in adults (cells/mm³)?
A decreased leukocyte number is also known as ______.
A decreased leukocyte number is also known as ______.
Which of the following materials is NOT required for performing a total leukocyte count?
Which of the following materials is NOT required for performing a total leukocyte count?
A bacterial infection typically leads to leukopenia.
A bacterial infection typically leads to leukopenia.
What is the function of the diluting fluid in a total leukocyte count?
What is the function of the diluting fluid in a total leukocyte count?
An increased number of leukocytes in the blood is referred to as ______.
An increased number of leukocytes in the blood is referred to as ______.
Which condition is LEAST likely to cause leukocytosis?
Which condition is LEAST likely to cause leukocytosis?
The Sahli pipette is used to measure 2 ml of blood sample.
The Sahli pipette is used to measure 2 ml of blood sample.
What is the role of glacial acetic acid in the diluting fluid?
What is the role of glacial acetic acid in the diluting fluid?
The normal leukocyte count for newborns is the same as for adults.
The normal leukocyte count for newborns is the same as for adults.
Which of the following conditions is associated with Leukopenia or Leukocytopenia?
Which of the following conditions is associated with Leukopenia or Leukocytopenia?
What is the role of the microscope in total leukocyte count?
What is the role of the microscope in total leukocyte count?
If a child between 1 to 10 years has a leukocyte count of 15,000/c.mm, this would be considered ______.
If a child between 1 to 10 years has a leukocyte count of 15,000/c.mm, this would be considered ______.
Flashcards
Total Leukocyte Count
Total Leukocyte Count
Counting the number of different types of leukocytes in one cubic millimeter of circulating blood.
Principle of Leukocyte Count
Principle of Leukocyte Count
Blood is diluted 20 times, lysing red blood cells and leaving white blood cells for counting.
Materials required for Leukocyte Count
Materials required for Leukocyte Count
Includes blood sample, Neubauer chamber, Sahli pipette, Khan tube, microscope and 2% glacial acetic acid
Diluting Fluid Composition
Diluting Fluid Composition
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Normal Adult Leukocyte Value
Normal Adult Leukocyte Value
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Leukocytosis
Leukocytosis
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Causes of Leukocytosis
Causes of Leukocytosis
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Leukopenia
Leukopenia
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Causes of Leukopenia
Causes of Leukopenia
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Study Notes
- Total leukocyte count involves counting the different types of leukocytes in one cubic millimeter of circulating blood.
- Blood is diluted 20 times with a white blood cell diluting fluid.
- Diluting fluid causes the lysis of red blood cells, leaving the white blood cells.
Materials required
- Blood sample
- Improved Neubauer chamber with cover slide
- Sahli pipette (0.02 ml)
- Khan tube
- Microscope
- 2% glacial acetic acid as diluting fluid
Diluting fluid preparation
- Diluting fluid is prepared with 2% glacial acetic acid and a drop of methylene blue.
- Methylene blue stains the nuclei of WBC with a blue color.
Normal leukocyte values
- Adult: 4000-11000/c.mm
- Children (1-10): 4,500-13,000/c.mm
- Newborn: 10,000-25000/c.mm
Leukocytosis
- Leukocytosis indicates an increased number of leukocytes.
- Leukocytosis can occur in acute inflammatory processes.
- Leukocytosis can occur in to bacterial, fungal, and parasitic infections.
- Leukemia can cause Leukocytosis.
- Cancer can cause Leukocytosis.
- Exposure to chemicals can result in Leukocytosis.
Leukopenia or Leukocytopenia
- Leukopenia indicates a decreased number of leukocytes.
- Viral infections can cause Leukopenia.
- Chemotherapy can cause Leukopenia.
- Radiotherapy can cause Leukopenia.
- Aplastic anemia can cause Leukopenia.
- Mylofibrosis can cause Leukopenia.
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