Tissue Preparation Process

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Match the tissue preparation steps with their correct order:

Fixation = 1st step Processing = 2nd step Embedding = 3rd step Sectioning = 4th step

Match the following actions with their importance in tissue preparation:

Cross checking for patient details = Ensuring correct identification Processing (dehydration, clearing, infiltration) = Preparing tissue for further steps Embedding = Preparing tissue for sectioning Mounting = Finalizing preparation for microscopic viewing

Match the following terms with their definitions:

Fixative = Chemical preserving tissue structure Dehydration = Removal of water from tissue Sectioning = Cutting tissue into thin slices Mounting = Placing tissue on a slide for viewing

Match the important checks with their purpose in tissue preparation:

Verifying test requisition form = Ensuring correct testing is performed Delivery to 'trimming' area = Preparing tissue for sectioning Cross checking for patient name and history = Avoiding errors in identification Receiving sample in fixative or fresh = Determining initial state of tissue Signup and view all the answers

Match the sources with their description of tissue preparation:

Apz_Grossing.(2021, January 28).Histology - Grossing station. = Describes steps involved in grossing specimens Klatt, E.C., MD.(n.d.).Histotechniques. = Provides information on histological specimen processing techniques Signup and view all the answers

Match the type of fixation with its description:

Heat fixation = Preserves overall morphology but not internal structures, used for single-cell organisms Freezing = Provides rapid results but lacks fine details, preservation occurs in milliseconds Chemical Fixation = Preserves structures as close to living tissue as possible, requires a stabilizing fixative Formalin Fixation = Most commonly used fixative in histology, neutral buffered formaldehyde in water Signup and view all the answers

Match the purpose of fixation with its description:

Preserving cells and tissue components = Objective is to keep them as close to normal as possible, prepare thin and stained sections Disabling proteolytic enzymes = Fixative acts to prevent digestion or damage by intrinsic biomolecules Increasing mechanical strength = Fixative alters cells/tissues on a molecular level to enhance stability during processing Protecting from extrinsic damage = Fixative shields the sample from external harm, toxic to common microorganisms Signup and view all the answers

Match the fixation protocol with its use case:

Immunohistochemistry = 'Quick fix' method using cold formalin for around 24 hours, prevents antibody binding Heat fixation = Mainly used for single-cell organisms like bacteria and archaea on microscope slides Freezing fixation = Preserves structures hydrated and close to native state, lacks fine details Chemical fixation = Stabilizes proteins, nucleic acids, and microsubstances using a fixative making them insoluble Signup and view all the answers

Match the type of tissue preparation with its outcome:

Embedding in paraffin = Followed by cutting into thin slices using a microtome for further analysis Staining and coverslipping = Occurs after cutting into thin slices, enhances visualization under microscopy Cassette placement = For tissue fixation before embedding in paraffin for preservation Fresh lung specimen in formalin container = Initial step before embedding in paraffin for preservation Signup and view all the answers

Match the fixative with its effect:

Neutral Buffered Formalin = Commonly used fixative that preserves tissues and cells in histology Paraformaldehyde = Another common fixative that stabilizes proteins and nucleic acids Osmium tetroxide = Not mentioned in the text but known to be a fixative used primarily for electron microscopy Glutaraldehyde = A fixative known for cross-linking proteins and preserving cell ultrastructure Signup and view all the answers