Tissue Preparation Process

10 Questions

Match the tissue preparation steps with their correct order:

Fixation = 1st step Processing = 2nd step Embedding = 3rd step Sectioning = 4th step

Match the following actions with their importance in tissue preparation:

Cross checking for patient details = Ensuring correct identification Processing (dehydration, clearing, infiltration) = Preparing tissue for further steps Embedding = Preparing tissue for sectioning Mounting = Finalizing preparation for microscopic viewing

Match the following terms with their definitions:

Fixative = Chemical preserving tissue structure Dehydration = Removal of water from tissue Sectioning = Cutting tissue into thin slices Mounting = Placing tissue on a slide for viewing

Match the important checks with their purpose in tissue preparation:

Verifying test requisition form = Ensuring correct testing is performed Delivery to 'trimming' area = Preparing tissue for sectioning Cross checking for patient name and history = Avoiding errors in identification Receiving sample in fixative or fresh = Determining initial state of tissue

Match the sources with their description of tissue preparation:

Apz_Grossing.(2021, January 28).Histology - Grossing station. = Describes steps involved in grossing specimens Klatt, E.C., MD.(n.d.).Histotechniques. = Provides information on histological specimen processing techniques

Match the type of fixation with its description:

Heat fixation = Preserves overall morphology but not internal structures, used for single-cell organisms Freezing = Provides rapid results but lacks fine details, preservation occurs in milliseconds Chemical Fixation = Preserves structures as close to living tissue as possible, requires a stabilizing fixative Formalin Fixation = Most commonly used fixative in histology, neutral buffered formaldehyde in water

Match the purpose of fixation with its description:

Preserving cells and tissue components = Objective is to keep them as close to normal as possible, prepare thin and stained sections Disabling proteolytic enzymes = Fixative acts to prevent digestion or damage by intrinsic biomolecules Increasing mechanical strength = Fixative alters cells/tissues on a molecular level to enhance stability during processing Protecting from extrinsic damage = Fixative shields the sample from external harm, toxic to common microorganisms

Match the fixation protocol with its use case:

Immunohistochemistry = 'Quick fix' method using cold formalin for around 24 hours, prevents antibody binding Heat fixation = Mainly used for single-cell organisms like bacteria and archaea on microscope slides Freezing fixation = Preserves structures hydrated and close to native state, lacks fine details Chemical fixation = Stabilizes proteins, nucleic acids, and microsubstances using a fixative making them insoluble

Match the type of tissue preparation with its outcome:

Embedding in paraffin = Followed by cutting into thin slices using a microtome for further analysis Staining and coverslipping = Occurs after cutting into thin slices, enhances visualization under microscopy Cassette placement = For tissue fixation before embedding in paraffin for preservation Fresh lung specimen in formalin container = Initial step before embedding in paraffin for preservation

Match the fixative with its effect:

Neutral Buffered Formalin = Commonly used fixative that preserves tissues and cells in histology Paraformaldehyde = Another common fixative that stabilizes proteins and nucleic acids Osmium tetroxide = Not mentioned in the text but known to be a fixative used primarily for electron microscopy Glutaraldehyde = A fixative known for cross-linking proteins and preserving cell ultrastructure

Learn about the steps and importance of preparing tissues for scientific or diagnostic purposes. Understand why fresh tissue specimens need to undergo significant alterations before being viewed under a microscope.