Questions and Answers
Which paraffin wax substitute is correctly matched to its melting point?
What is the first and most important procedure in the preparation of tissues for microscopic examination?
Fixation
Which of the following describes the reduction of body temperature and loss of skin elasticity that occurs after death?
Algor mortis
Which of the following is not an adhesive?
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Which of the following is not a clearing agent?
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What term refers to the ratio of speed of light in air and speed of light in a specific medium?
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Who serves as the chairman of the council for Medical Technology Education?
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Which of the following is used for the localization of vaginal adenosis?
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What is the best probable cause of a fixation problem?
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What is the ideal volume or ratio of fixative to specimen?
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What is the reversible change of one cell type to another?
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What is the ideal thickness of tissue paraffin sections for routine histologic sectioning?
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Study Notes
Tissue Preparation Process
- Paraplast: A paraffin wax substitute with a melting point of 56-57°C.
- Fixation: The critical first step in tissue preparation for microscopic examination, preserving the structure and composition of the tissue.
- Adhesives: Gelatin formaldehyde is identified as not being an adhesive.
Clearing Agents
- Clearing agent: Acetone is acknowledged as not a clearing agent in histological processing.
Post-Mortem Changes
- Algor mortis: Refers to the reduction of body temperature and loss of skin elasticity post-death.
Optical Properties
- Refractive index: Represents the ratio of the speed of light in air compared to its speed in a specific medium, important for understanding light behavior in tissues.
Medical Education Governance
- Chairman of the Council for Medical Technology Education: The position is held by the CHED Secretary, overseeing standards in medical technology education.
Fixation Issues
- Common fixation problem: The best probable cause is failure to fix the tissue immediately, which can compromise tissue integrity.
Histological Techniques
- Vaginal adenosis localization: Achieved through a 4 quadrant vaginal scrape for better sample analysis.
- Fixative to specimen ratio: An ideal ratio of 20:1 is essential for effective fixation of tissue samples.
- Tissue section thickness: Routine histological sections should be 5-10 micrometers thick for optimal examination.
- Hyperplasia: Defined as the reversible change where one cell type transforms into another, often as a response to stimuli.
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Description
Test your knowledge on tissue preparation techniques and histology. This quiz covers important procedures, materials used in histology, and common misconceptions in the field. Explore key concepts related to the preparation of tissues for microscopic examination.
