Tissue Impregnation Quiz
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Questions and Answers

Which of the following statements about alcohol is correct?

  • Alcohol evaporates quickly
  • Alcohol can dehydrate tissues (correct)
  • Alcohol is non-polar and does not mix with water
  • Adding water to alcohol reduces dehydration

    • What happens when alcohol is added to chicharon?

  • It becomes milky
  • It dissolves completely
  • It becomes non-toxic
  • It turns turbid (correct)

    • What is the best solvent for chicharon?

  • Gasoline
  • Water
  • Alcohol
  • Ether (correct)

    • Which principle explains why alcohol mixes with water?

    <p>Like dissolves like</p> Signup and view all the answers

    Which dehydrating agent is considered the fastest?

    <p>Acetone</p> Signup and view all the answers

    Which dehydrating agent is recommended for blood films?

    <p>Methyl</p> Signup and view all the answers

    Which clearing agent should not be used due to tissue shrinkage and hardening?

    <p>Dioxane</p> Signup and view all the answers

    Which clearing agent is miscible with alcohol and paraffin?

    <p>Alcohol</p> Signup and view all the answers

    Which clearing agent is not recommended for the nervous system and lymph nodes?

    <p>chloroform</p> Signup and view all the answers

    Which clearing agent is completely removed from the tissue and replaced by a medium that fills all the tissue cavities?

    <p>THF</p> Signup and view all the answers

    Which clearing agent is slow acting but used for double embedding?

    <p>Aniline oil</p> Signup and view all the answers

    Which clearing agent is toxic to the liver upon prolonged inhalation and does not make tissues transparent?

    <p>Chloroform</p> Signup and view all the answers

    Which type of wax is recommended for impregnation without prior clearing?

    <p>Ester Wax</p> Signup and view all the answers

    Which type of wax is recommended for enzyme histochemistry procedures?

    <p>Water Soluble Wax</p> Signup and view all the answers

    What is the purpose of impregnation in the embedding process?

    <p>To replace the clearing agent with a medium that fills tissue cavities</p> Signup and view all the answers

    Why is the use of celloidin recommended for specimens with large hollow cavities?

    <p>It prevents tissue crumbling</p> Signup and view all the answers

    Which of the following is NOT a type of impregnation and embedding medium mentioned in the text?

    <p>Nitrocellulose</p> Signup and view all the answers

    What is the recommended temperature for paraffin wax impregnation?

    <p>56°C to 58°C</p> Signup and view all the answers

    Which of the following is an advantage of paraffin wax impregnation?

    <p>Allows for rapid preparation of sections</p> Signup and view all the answers

    What is the purpose of embedding medium in tissue processing?

    <p>To provide support for the tissue during sectioning</p> Signup and view all the answers

    Which of the following tissues require long immersion for proper support during impregnation?

    <p>Bones</p> Signup and view all the answers

    What happens to tissues that are inadequately impregnated?

    <p>They become soft and shrunken</p> Signup and view all the answers

    Why is paraffin processing not recommended for fatty tissues?

    <p>Paraffin dissolves and removes fat from the tissues</p> Signup and view all the answers

    Which wax is most commonly used in the laboratory for routine work?

    <p>56°C wax</p> Signup and view all the answers

    Which step in the tissue processing schedule takes the longest amount of time?

    <p>Wax (2)</p> Signup and view all the answers

    What is the purpose of the delay mechanism in the processing machine?

    <p>To account for processing time exceeding 24 hours</p> Signup and view all the answers

    Which tissues are particularly recommended for vacuum infiltration?

    <p>Connective tissues, decalcified bones, and spleen</p> Signup and view all the answers

    What indicates that the paraffin wax needs to be changed?

    <p>The presence of any odor in the clearing agent</p> Signup and view all the answers

    What is the recommended setting for wax bath thermostats?

    <p>3 degrees above the melting point of the wax</p> Signup and view all the answers

    How does vacuum infiltration facilitate tissue processing?

    <p>All of the above</p> Signup and view all the answers

    Which step in the tissue processing schedule takes the longest amount of time?

    <p>Wax (2)</p> Signup and view all the answers

    What is the purpose of embedding medium in tissue processing?

    <p>To facilitate wax penetration of the tissue</p> Signup and view all the answers

    Which of the following tissues require long immersion for proper support during impregnation?

    <p>Decalcified bones</p> Signup and view all the answers

    What is the recommended setting for wax bath thermostats?

    <p>3 degrees above the melting point of the wax</p> Signup and view all the answers

    Why is paraffin processing not recommended for fatty tissues?

    <p>Paraffin wax does not penetrate fatty tissues well</p> Signup and view all the answers

    What happens to tissues that are inadequately impregnated?

    <p>They become hard and brittle</p> Signup and view all the answers

    What is the purpose of the delay mechanism in the processing machine?

    <p>To provide a delay in instances where processing time may exceed 24 hours</p> Signup and view all the answers

    Which clearing agent is not recommended for the nervous system and lymph nodes?

    <p>Xylene or Toluene (2)</p> Signup and view all the answers

    Which clearing agent is toxic to the liver upon prolonged inhalation and does not make tissues transparent?

    <p>Chloroform (2)</p> Signup and view all the answers

    Which of the following is an advantage of paraffin wax impregnation?

    <p>Promotes rapid wax penetration of the tissue</p> Signup and view all the answers

    Study Notes

    Tissue Processing

    • When alcohol is added to chicharon, it helps to dehydrate it.

    Solvents and Clearing Agents

    • The best solvent for chicharon is alcohol.
    • Like dissolves like, which explains why alcohol mixes with water.
    • The fastest dehydrating agent is not specified in the text, but it is not recommended for blood films.
    • Xylene is not recommended for the nervous system and lymph nodes.
    • Xylene is toxic to the liver upon prolonged inhalation and does not make tissues transparent.
    • Toluene is miscible with alcohol and paraffin.

    Embedding and Impregnation

    • Paraffin wax is recommended for impregnation without prior clearing.
    • No prior clearing is needed for enzyme histochemistry procedures.
    • The purpose of impregnation is to fill all tissue cavities with a medium.
    • Celloidin is recommended for specimens with large hollow cavities.
    • The purpose of embedding medium is to provide support to tissues.
    • Tissues that are inadequately impregnated will not have proper support.
    • Paraffin processing is not recommended for fatty tissues.
    • Paraffin wax is most commonly used in the laboratory for routine work.

    Processing Schedule

    • The longest step in the tissue processing schedule is not specified in the text.
    • The delay mechanism in the processing machine allows for adequate time for processing.
    • Vacuum infiltration facilitates tissue processing by removing air from the tissue.

    Temperature and Settings

    • The recommended temperature for paraffin wax impregnation is not specified in the text.
    • The recommended setting for wax bath thermostats is around 58-60°C.
    • Paraffin wax needs to be changed when it becomes dirty or discolored.

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    Description

    Quiz: Test your knowledge on tissue impregnation and its effects Description: Take this quiz to assess your understanding of tissue impregnation and its impact on the retention of clearing agents. Explore topics such as tissue softening, shrinkage, and the challenges faced when infiltrating certain tissues. Test your knowledge on proper immersion techniques and the consequences of inadequate impregnation.

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