40 Questions
Which of the following statements about alcohol is correct?
Alcohol can dehydrate tissues
What happens when alcohol is added to chicharon?
It turns turbid
What is the best solvent for chicharon?
Ether
Which principle explains why alcohol mixes with water?
Like dissolves like
Which dehydrating agent is considered the fastest?
Acetone
Which dehydrating agent is recommended for blood films?
Methyl
Which clearing agent should not be used due to tissue shrinkage and hardening?
Dioxane
Which clearing agent is miscible with alcohol and paraffin?
Alcohol
Which clearing agent is not recommended for the nervous system and lymph nodes?
chloroform
Which clearing agent is completely removed from the tissue and replaced by a medium that fills all the tissue cavities?
THF
Which clearing agent is slow acting but used for double embedding?
Aniline oil
Which clearing agent is toxic to the liver upon prolonged inhalation and does not make tissues transparent?
Chloroform
Which type of wax is recommended for impregnation without prior clearing?
Ester Wax
Which type of wax is recommended for enzyme histochemistry procedures?
Water Soluble Wax
What is the purpose of impregnation in the embedding process?
To replace the clearing agent with a medium that fills tissue cavities
Why is the use of celloidin recommended for specimens with large hollow cavities?
It prevents tissue crumbling
Which of the following is NOT a type of impregnation and embedding medium mentioned in the text?
Nitrocellulose
What is the recommended temperature for paraffin wax impregnation?
56°C to 58°C
Which of the following is an advantage of paraffin wax impregnation?
Allows for rapid preparation of sections
What is the purpose of embedding medium in tissue processing?
To provide support for the tissue during sectioning
Which of the following tissues require long immersion for proper support during impregnation?
Bones
What happens to tissues that are inadequately impregnated?
They become soft and shrunken
Why is paraffin processing not recommended for fatty tissues?
Paraffin dissolves and removes fat from the tissues
Which wax is most commonly used in the laboratory for routine work?
56°C wax
Which step in the tissue processing schedule takes the longest amount of time?
Wax (2)
What is the purpose of the delay mechanism in the processing machine?
To account for processing time exceeding 24 hours
Which tissues are particularly recommended for vacuum infiltration?
Connective tissues, decalcified bones, and spleen
What indicates that the paraffin wax needs to be changed?
The presence of any odor in the clearing agent
What is the recommended setting for wax bath thermostats?
3 degrees above the melting point of the wax
How does vacuum infiltration facilitate tissue processing?
All of the above
Which step in the tissue processing schedule takes the longest amount of time?
Wax (2)
What is the purpose of embedding medium in tissue processing?
To facilitate wax penetration of the tissue
Which of the following tissues require long immersion for proper support during impregnation?
Decalcified bones
What is the recommended setting for wax bath thermostats?
3 degrees above the melting point of the wax
Why is paraffin processing not recommended for fatty tissues?
Paraffin wax does not penetrate fatty tissues well
What happens to tissues that are inadequately impregnated?
They become hard and brittle
What is the purpose of the delay mechanism in the processing machine?
To provide a delay in instances where processing time may exceed 24 hours
Which clearing agent is not recommended for the nervous system and lymph nodes?
Xylene or Toluene (2)
Which clearing agent is toxic to the liver upon prolonged inhalation and does not make tissues transparent?
Chloroform (2)
Which of the following is an advantage of paraffin wax impregnation?
Promotes rapid wax penetration of the tissue
Quiz: Test your knowledge on tissue impregnation and its effects Description: Take this quiz to assess your understanding of tissue impregnation and its impact on the retention of clearing agents. Explore topics such as tissue softening, shrinkage, and the challenges faced when infiltrating certain tissues. Test your knowledge on proper immersion techniques and the consequences of inadequate impregnation.
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