Tissue Fixation and Dehydration Techniques

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Questions and Answers

What are the goals of tissue fixation?

Enhancing tissue coloration for better visual inspection.

Facilitating the growth of microorganisms.

Slowing down tissue metabolism and cellular activities.

Preservation of tissue structure, prevention of decay, facilitating microscopic examination, preparation for molecular analysis, and archiving. (correct)

What are the characteristics of a good fixative?

Variable tissue penetration, variable reaction speed, moderate structural alterations, variable safety, variable cost.

No tissue penetration, no reaction, no structural alterations, not applicable for use, no cost.

Slow tissue penetration, delayed reaction, significant structural alterations, dangerous to use, high cost.

Rapid tissue penetration, quick reaction, minimal structural alterations, safe to use, low cost. (correct)

Why is it important for a 10% formalin-buffered solution to have a pH range of 7.2 to 7.4 for tissue preservation?

To ensure the stability of the fixative and effective tissue preservation. (correct)

To improve tissue coloration.

To accelerate tissue degradation.

To make the solution less expensive.

What factors affect tissue fixation?

All of the above. (D) Signup and view all the answers

What is the purpose of the dehydration process in tissue preparation?

To replace water in tissues with non-polar substances. (B) Signup and view all the answers

What is the purpose of using intermediate fluids in tissue preparation?

To facilitate the transition from dehydration to paraffin infiltration. (B) Signup and view all the answers

Select the characteristic components of granulation tissue:

Fibroblasts, loose connective tissue, new capillaries, inflammatory cells (A) Signup and view all the answers

Which change is typical of cell aging?

Shortening of telomeres (C) Signup and view all the answers

What is characteristic of myocardial infarction from a pathological point of view?

Coagulative necrosis (B), Coagulative necrosis (D) Signup and view all the answers

What characterizes Lynch syndrome?

Increased colorectal cancer (D) Signup and view all the answers

Which of the following factors is NOT associated with the development of atherosclerosis?

High vit C level (B) Signup and view all the answers

Select the false statement regarding miRNA:

They encode proteins (A) Signup and view all the answers

Match the following programming languages with their primary usage:

Python = General-purpose programming JavaScript = Client-side scripting for web applications SQL = Database queries CSS = Styling web pages Signup and view all the answers

Study Notes

Tissue Fixation Goals

Slowing down tissue metabolism and cellular activity
Enhancing tissue coloration for better visual inspection
Preserving tissue structure, preventing decay, enabling microscopic examination, and preparing for molecular analysis and archiving

Good Fixative Characteristics

Rapid tissue penetration
Quick reaction
Minimal structure alteration
Safe to use
Low cost

Tissue Dehydration Purpose

Replacing water with nonpolar substances
Preserving tissue structure
Enhancing tissue staining

Tissue Fixation Factors

Time and temperature
Material type (bone, soft tissue, cartilage)
Chemical properties (pH) of the fixative

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Description

This quiz covers the essential goals and characteristics of tissue fixation, including the importance of dehydration. Explore the factors affecting tissue preservation, staining, and microscopic examination. Perfect for students in histology or biology courses.