Tissue Clearing and Dehydration Methods
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Questions and Answers

What is the recommended duration for xylene two changes?

  • 20 minutes to one hour (correct)
  • 10 minutes to 30 minutes
  • Two hours to three hours
  • One hour to two hours

    • What is a characteristic of chloroform that makes it suitable for certain tissues?

  • It is a slowly penetrating transition solvent (correct)
  • It is a highly volatile solvent
  • It is a cheap, lightweight solvent
  • It is a rapidly penetrating transition solvent

    • What is a limitation of using chloroform?

  • It attacks some plastics and sealants (correct)
  • It is a highly volatile solvent
  • It is a slowly penetrating transition solvent
  • It is a cheap, lightweight solvent

    • What is a characteristic of esters that makes them suitable for manual processing?

    <p>Tissues may be left in them for extended periods without affecting the tissue or organ</p> Signup and view all the answers

    What is required to eliminate esters from paraffin wax?

    <p>One or two brief changes of toluene or similar solvent</p> Signup and view all the answers

    What is a characteristic of toluene?

    <p>It produces less damage with prolonged immersion</p> Signup and view all the answers

    Why was chloroform replaced in tissue processing?

    <p>It is a health hazard</p> Signup and view all the answers

    What is the primary function of dehydration in tissue processing?

    <p>To remove fixative and water</p> Signup and view all the answers

    What is the purpose of using paraplast in tissue processing?

    <p>To make the paraffin blocks easier to cut</p> Signup and view all the answers

    What is the sequence of alcohol concentrations used in dehydration?

    <p>70% to 95% to absolute alcohol</p> Signup and view all the answers

    What is the term for the process of immersing tissues in increasing concentrations of hydrophilic or water miscible fluids?

    <p>Dilution dehydration</p> Signup and view all the answers

    What determines the choice of paraffin wax with a specific melting point?

    <p>All of the above</p> Signup and view all the answers

    What is a characteristic of methyl salicylate compared to methyl benzoate?

    <p>It hardens less and has a more pleasant odor</p> Signup and view all the answers

    What is a limitation of using chlorinated hydrocarbons?

    <p>They can decompose to form phosgene and hydrochloric acid</p> Signup and view all the answers

    What is a drawback of using citrus fruit oils as clearing agents?

    <p>They can leach out dyes from sections</p> Signup and view all the answers

    What is the purpose of impregnation in tissue preparation?

    <p>To saturate tissue cavities and cells with a supporting substance</p> Signup and view all the answers

    What is a characteristic of chlorinated hydrocarbons?

    <p>They are colorless and have a sweet odor</p> Signup and view all the answers

    What is a type of embedding medium used in impregnation?

    <p>Paraffin wax</p> Signup and view all the answers

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