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Tissue Clearing and Dehydration Methods

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Questions and Answers

What is the recommended duration for xylene two changes?

20 minutes to one hour (correct)

10 minutes to 30 minutes

Two hours to three hours

One hour to two hours

What is a characteristic of chloroform that makes it suitable for certain tissues?

It is a slowly penetrating transition solvent (correct)

It is a highly volatile solvent

It is a cheap, lightweight solvent

It is a rapidly penetrating transition solvent

What is a limitation of using chloroform?

It attacks some plastics and sealants (correct)

It is a highly volatile solvent

It is a slowly penetrating transition solvent

It is a cheap, lightweight solvent

What is a characteristic of esters that makes them suitable for manual processing?

Tissues may be left in them for extended periods without affecting the tissue or organ Signup and view all the answers

What is required to eliminate esters from paraffin wax?

One or two brief changes of toluene or similar solvent Signup and view all the answers

What is a characteristic of toluene?

It produces less damage with prolonged immersion Signup and view all the answers

Why was chloroform replaced in tissue processing?

It is a health hazard Signup and view all the answers

What is the primary function of dehydration in tissue processing?

To remove fixative and water Signup and view all the answers

What is the purpose of using paraplast in tissue processing?

To make the paraffin blocks easier to cut Signup and view all the answers

What is the sequence of alcohol concentrations used in dehydration?

70% to 95% to absolute alcohol Signup and view all the answers

What is the term for the process of immersing tissues in increasing concentrations of hydrophilic or water miscible fluids?

Dilution dehydration Signup and view all the answers

What determines the choice of paraffin wax with a specific melting point?

All of the above Signup and view all the answers

What is a characteristic of methyl salicylate compared to methyl benzoate?

It hardens less and has a more pleasant odor Signup and view all the answers

What is a limitation of using chlorinated hydrocarbons?

They can decompose to form phosgene and hydrochloric acid Signup and view all the answers

What is a drawback of using citrus fruit oils as clearing agents?

They can leach out dyes from sections Signup and view all the answers

What is the purpose of impregnation in tissue preparation?

To saturate tissue cavities and cells with a supporting substance Signup and view all the answers

What is a characteristic of chlorinated hydrocarbons?

They are colorless and have a sweet odor Signup and view all the answers

What is a type of embedding medium used in impregnation?

Paraffin wax Signup and view all the answers