18 Questions
What is the recommended duration for xylene two changes?
20 minutes to one hour
What is a characteristic of chloroform that makes it suitable for certain tissues?
It is a slowly penetrating transition solvent
What is a limitation of using chloroform?
It attacks some plastics and sealants
What is a characteristic of esters that makes them suitable for manual processing?
Tissues may be left in them for extended periods without affecting the tissue or organ
What is required to eliminate esters from paraffin wax?
One or two brief changes of toluene or similar solvent
What is a characteristic of toluene?
It produces less damage with prolonged immersion
Why was chloroform replaced in tissue processing?
It is a health hazard
What is the primary function of dehydration in tissue processing?
To remove fixative and water
What is the purpose of using paraplast in tissue processing?
To make the paraffin blocks easier to cut
What is the sequence of alcohol concentrations used in dehydration?
70% to 95% to absolute alcohol
What is the term for the process of immersing tissues in increasing concentrations of hydrophilic or water miscible fluids?
Dilution dehydration
What determines the choice of paraffin wax with a specific melting point?
All of the above
What is a characteristic of methyl salicylate compared to methyl benzoate?
It hardens less and has a more pleasant odor
What is a limitation of using chlorinated hydrocarbons?
They can decompose to form phosgene and hydrochloric acid
What is a drawback of using citrus fruit oils as clearing agents?
They can leach out dyes from sections
What is the purpose of impregnation in tissue preparation?
To saturate tissue cavities and cells with a supporting substance
What is a characteristic of chlorinated hydrocarbons?
They are colorless and have a sweet odor
What is a type of embedding medium used in impregnation?
Paraffin wax
Learn about the properties and uses of methyl salicylate and chlorinated hydrocarbons in tissue clearing and dehydration processes. Understand their advantages and disadvantages in histological preparations.
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