Histology Tissue Processing Quiz

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What is a potential consequence of insufficient dehydration during tissue clearing?

Mushy tissue blocks (correct)

Formation of air bubbles in tissue

Enhanced tissue visibility

Increased tissue brittleness

Which characteristic is essential for a clearing agent used in histology?

Low cost

Miscibility with tissue and infiltrating media (correct)

Pleasant odor

Non-toxic nature

What is a disadvantage of using universal solvents for dehydration and clearing?

Prolonged processing time

Incompatibility with embedding materials

Increased tissue damage

Toxic and unpleasant smell (correct)

Which of the following additives is used specifically to soften hard tissues?

Phenol Signup and view all the answers

What is one important criterion when selecting a clearing agent in tissue processing?

It allows for speedy removal of dehydrant Signup and view all the answers

What is the primary reason for performing dehydration in a step-wise manner rather than a single step?

To prevent tissue distortion, especially in delicate tissues Signup and view all the answers

Which of the following is NOT an advantage of ethyl alcohol as a dehydrating agent?

Promotes significant shrinkage when used in high concentrations Signup and view all the answers

What is the maximum concentration of alcohol recommended to start with in a closed processor to avoid buffer precipitation?

60% Signup and view all the answers

Which statement best describes the characteristics of graded alcohols used in dehydration?

They include concentrations such as 50%, 60%, 70%, 80%, 90%, and 100% Signup and view all the answers

What is a significant drawback of using microwaves for tissue dehydration?

Microwave dehydration may lead to uneven tissue shrinkage Signup and view all the answers

Which statement accurately describes a disadvantage of ethyl alcohol?

Long exposure can lead to shrinking and hardening of materials. Signup and view all the answers

What is a unique hazard associated with methyl and isopropanol compared to ethyl alcohol?

They can cause serious poisoning. Signup and view all the answers

Which characteristic makes isopropanol a preferable option in certain situations?

It has less potential for shrinkage and hardening. Signup and view all the answers

Which of the following statements is true regarding the disposal of high-concentration alcohols?

Only alcohols above 24% should go to hazardous waste management. Signup and view all the answers

What is a common misconception about using ethanol in comparison to isopropanol?

Ethanol has more governmental restrictions than isopropanol. Signup and view all the answers

Which of the following statements is true regarding the disadvantages of using Isopropanol?

It is not suitable for use with nitrocellulose. Signup and view all the answers

What is the primary advantage of using Acetone compared to other dehydrating agents?

It is less expensive and offers rapid dehydration. Signup and view all the answers

Which of the following represents a significant risk associated with Methyl Alcohol?

Overexposure can result in severe health consequences like blindness. Signup and view all the answers

What is the main advantage of Denatured Alcohol compared to standard ethanol?

It eliminates security measures and avoids record-keeping. Signup and view all the answers

What must be considered when using Acetone as a dehydrating agent?

Grading of acetone to xylene is necessary for best processing. Signup and view all the answers