Histology Tissue Processing Quiz
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Questions and Answers

What is a potential consequence of insufficient dehydration during tissue clearing?

  • Mushy tissue blocks (correct)
  • Formation of air bubbles in tissue
  • Enhanced tissue visibility
  • Increased tissue brittleness

Which characteristic is essential for a clearing agent used in histology?

  • Low cost
  • Miscibility with tissue and infiltrating media (correct)
  • Pleasant odor
  • Non-toxic nature

What is a disadvantage of using universal solvents for dehydration and clearing?

  • Prolonged processing time
  • Incompatibility with embedding materials
  • Increased tissue damage
  • Toxic and unpleasant smell (correct)

Which of the following additives is used specifically to soften hard tissues?

<p>Phenol (D)</p> Signup and view all the answers

What is one important criterion when selecting a clearing agent in tissue processing?

<p>It allows for speedy removal of dehydrant (D)</p> Signup and view all the answers

What is the primary reason for performing dehydration in a step-wise manner rather than a single step?

<p>To prevent tissue distortion, especially in delicate tissues (D)</p> Signup and view all the answers

Which of the following is NOT an advantage of ethyl alcohol as a dehydrating agent?

<p>Promotes significant shrinkage when used in high concentrations (A)</p> Signup and view all the answers

What is the maximum concentration of alcohol recommended to start with in a closed processor to avoid buffer precipitation?

<p>60% (A)</p> Signup and view all the answers

Which statement best describes the characteristics of graded alcohols used in dehydration?

<p>They include concentrations such as 50%, 60%, 70%, 80%, 90%, and 100% (D)</p> Signup and view all the answers

What is a significant drawback of using microwaves for tissue dehydration?

<p>Microwave dehydration may lead to uneven tissue shrinkage (D)</p> Signup and view all the answers

Which statement accurately describes a disadvantage of ethyl alcohol?

<p>Long exposure can lead to shrinking and hardening of materials. (C)</p> Signup and view all the answers

What is a unique hazard associated with methyl and isopropanol compared to ethyl alcohol?

<p>They can cause serious poisoning. (A)</p> Signup and view all the answers

Which characteristic makes isopropanol a preferable option in certain situations?

<p>It has less potential for shrinkage and hardening. (C)</p> Signup and view all the answers

Which of the following statements is true regarding the disposal of high-concentration alcohols?

<p>Only alcohols above 24% should go to hazardous waste management. (B)</p> Signup and view all the answers

What is a common misconception about using ethanol in comparison to isopropanol?

<p>Ethanol has more governmental restrictions than isopropanol. (D)</p> Signup and view all the answers

Which of the following statements is true regarding the disadvantages of using Isopropanol?

<p>It is not suitable for use with nitrocellulose. (B)</p> Signup and view all the answers

What is the primary advantage of using Acetone compared to other dehydrating agents?

<p>It is less expensive and offers rapid dehydration. (B)</p> Signup and view all the answers

Which of the following represents a significant risk associated with Methyl Alcohol?

<p>Overexposure can result in severe health consequences like blindness. (D)</p> Signup and view all the answers

What is the main advantage of Denatured Alcohol compared to standard ethanol?

<p>It eliminates security measures and avoids record-keeping. (D)</p> Signup and view all the answers

What must be considered when using Acetone as a dehydrating agent?

<p>Grading of acetone to xylene is necessary for best processing. (A)</p> Signup and view all the answers

Flashcards

Ethyl Alcohol Disadvantages

Ethyl alcohol is expensive due to taxes, prolonged exposure can damage materials, and record-keeping and theft prevention are issues.

Isopropanol Advantages

Isopropanol penetrates similarly to ethanol and is a good substitute for it, with less shrinkage and hardening.

Isopropanol Disadvantages

Isopropanol can't be used with celloidin, but generally, it has no government restrictions and is less expensive.

Alcohol Flammability

Alcohols, like ethanol, pose safety hazards related to flammability during use, storage, and disposal.

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Alcohol Toxicity

Ethyl alcohol is intoxicating, while methyl and isopropyl alcohols are poisonous. Mixing alcohols with oxidizing agents is dangerous.

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Dehydration in tissue processing

A process that removes water from tissue using reagents that attract water and repeated dilution of aqueous tissue fluids, removing aqueous fixatives.

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Dehydrating Agent Example

Ethanol (ethyl alcohol) is a common dehydrating agent. It's used in a step-by-step fashion, increasing its concentration.

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Graded Alcohols

Different concentrations of ethanol used in steps to dehydrate tissue, typically 50%, 60%, 70%, 80%, 90%, and 100% (absolute).

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Tissue distortion during dehydration

Dehydration in one step can cause tissue damage, especially in delicate tissues.

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Microwave Dehydration Advantage

Microwave dehydration can happen in one step due to rapid molecular movement and diffusion.

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Acetone's disadvantage for dehydration

Acetone requires a large volume (20 times the tissue volume), potentially causing shrinkage. It's also flammable and very volatile, needing good ventilation.

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Methyl Alcohol's risk

Overexposure to methyl alcohol can cause serious health problems, including blindness or death.

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Denatured Alcohol's advantage

Denatured alcohol works well as a solvent, avoiding taxes and record-keeping while retaining solvent properties.

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Isopropanol's limitation

Isopropanol isn't suitable for celloidin, nitrocellulose, or stains like Eosin, due to incompatibility and mild irritation.

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Key alcohol for final dehydration

The last alcohol used during dehydration is critical—it needs to effectively remove water, ensuring proper sample preparation for the following steps.

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Tissue Clearing Purpose

Tissue clearing removes alcohol and replaces it with a clearing agent to make tissues transparent for easier visualization.

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Clearing Agent Properties

A good clearing agent must mix well with the tissue, the surrounding media (like paraffin), and easily remove the alcohol.

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Excess Clearing Issues

Overusing a clearing agent can cause tissue to become brittle.

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Incomplete Dehydration

Tissue that isn't completely dehydrated before clearing will result in a soft, mushy block.

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Universal Solvent for Dehydration and Clearing

Substances like dioxane, tertiary butanol, and tetrahydrofuran can be used in a single step for both dehydration and clearing, but they may be toxic, expensive and have a bad smell.

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