Histology: Dehydration and Clearing Process
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Questions and Answers

What is the purpose of the dehydration process in tissue processing?

  • To remove the water content from the tissue using increasing concentrations of dehydrating agents (correct)
  • To fix the tissue with 10% formalin saline
  • To preserve the tissue for long-term storage
  • To transfer the tissue through a series of ascending gradient of Alcohol
  • How is 70% ethanol prepared for the dehydration process?

  • By mixing 90 ml of absolute alcohol with 10 ml of distilled water
  • By mixing 80 ml of absolute alcohol with 20 ml of distilled water
  • By mixing 70 ml of absolute alcohol with 20 ml of distilled water
  • By mixing 70 ml of absolute alcohol with 30 ml of distilled water (correct)
  • What happens to the tissue during the dehydration process?

  • The tissue becomes more flexible and transparent
  • The tissue is disintegrated by the dehydrating agents
  • Water is replaced by diffusion as the tissue is passed through increasing strength of alcohol (correct)
  • The tissue increases in volume due to the removal of water
  • Why is the dehydration process important in tissue processing?

    <p>To facilitate infiltration of embedding medium into the tissue</p> Signup and view all the answers

    What is the final concentration of alcohol used in the dehydration process?

    <p>95% ethanol</p> Signup and view all the answers

    What is the purpose of the clearing process in tissue processing?

    <p>To remove alcohol and replace it with Xylene</p> Signup and view all the answers

    What is the main function of an automatic tissue processor during the clearing stage?

    <p>Control the timings for dehydration and clearing</p> Signup and view all the answers

    Which substance is used to replace alcohol with in the clearing process?

    <p>Xylene</p> Signup and view all the answers

    Why is a solvent miscible with both the dehydrants and the infiltration medium used in the clearing process?

    <p>To facilitate the transition between dehydration and infiltration with embedding medium</p> Signup and view all the answers

    What is the purpose of the mechanical device in mechanical tissue processing?

    <p>To control the timings for dehydration and clearing</p> Signup and view all the answers

    The automatic tissue processor replaces water with alcohol during the dehydration process.

    <p>False</p> Signup and view all the answers

    Xylene is used to replace alcohol during the clearing process in tissue processing.

    <p>True</p> Signup and view all the answers

    Chloroform is not a clearing agent used in tissue processing.

    <p>False</p> Signup and view all the answers

    The main function of the mechanical device in mechanical tissue processing is to control the timings by a timer.

    <p>True</p> Signup and view all the answers

    During the clearing process, tissue is prepared for infiltration with dehydration solutions.

    <p>False</p> Signup and view all the answers

    During the dehydration process, the tissue is completely removed by passing the tissue through decreasing concentrations of dehydrating agents.

    <p>False</p> Signup and view all the answers

    80% ethanol is prepared by mixing 80 ml of absolute alcohol with 20 ml of distilled water.

    <p>True</p> Signup and view all the answers

    Dehydration involves replacing the water in the tissue with diffusion.

    <p>True</p> Signup and view all the answers

    70% ethanol is prepared by mixing 70 ml of absolute alcohol with 30 ml of distilled water.

    <p>False</p> Signup and view all the answers

    In the dehydration process, the tissue is first properly fixed with 5% formalin saline.

    <p>False</p> Signup and view all the answers

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