20 Questions
What is the purpose of the dehydration process in tissue processing?
To remove the water content from the tissue using increasing concentrations of dehydrating agents
How is 70% ethanol prepared for the dehydration process?
By mixing 70 ml of absolute alcohol with 30 ml of distilled water
What happens to the tissue during the dehydration process?
Water is replaced by diffusion as the tissue is passed through increasing strength of alcohol
Why is the dehydration process important in tissue processing?
To facilitate infiltration of embedding medium into the tissue
What is the final concentration of alcohol used in the dehydration process?
95% ethanol
What is the purpose of the clearing process in tissue processing?
To remove alcohol and replace it with Xylene
What is the main function of an automatic tissue processor during the clearing stage?
Control the timings for dehydration and clearing
Which substance is used to replace alcohol with in the clearing process?
Xylene
Why is a solvent miscible with both the dehydrants and the infiltration medium used in the clearing process?
To facilitate the transition between dehydration and infiltration with embedding medium
What is the purpose of the mechanical device in mechanical tissue processing?
To control the timings for dehydration and clearing
The automatic tissue processor replaces water with alcohol during the dehydration process.
False
Xylene is used to replace alcohol during the clearing process in tissue processing.
True
Chloroform is not a clearing agent used in tissue processing.
False
The main function of the mechanical device in mechanical tissue processing is to control the timings by a timer.
True
During the clearing process, tissue is prepared for infiltration with dehydration solutions.
False
During the dehydration process, the tissue is completely removed by passing the tissue through decreasing concentrations of dehydrating agents.
False
80% ethanol is prepared by mixing 80 ml of absolute alcohol with 20 ml of distilled water.
True
Dehydration involves replacing the water in the tissue with diffusion.
True
70% ethanol is prepared by mixing 70 ml of absolute alcohol with 30 ml of distilled water.
False
In the dehydration process, the tissue is first properly fixed with 5% formalin saline.
False
Learn about the dehydration process in tissue preparation for histology, involving the removal of water content by passing the tissue through increasing concentrations of dehydrating agents. Understand the importance of this step in tissue processing.
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