17 Questions
What is Reverse T3 (rT3) used to assess?
Borderline or conflicting laboratory results
What is Euthyroid sick syndrome also known as?
Non-thyroidal illness syndrome
What is a primary increase in the concentration of TBG seen in?
Pregnancy and estrogen therapy
What is a primary decrease in the concentration of TBG seen in?
Nephrotic syndrome, decreased protein production, and ingestion of certain drugs
Why do hospitalized patients, especially critically ill patients, often have abnormal thyroid function tests?
Due to illness decreasing 5-monodeiodinase activity, leading to less T4 being converted to active T3
What is the typical laboratory pattern seen in non-thyroidal illness?
Low total T4, free T4, and TSH
What is the principle behind Chemiluminescent immunoassay?
Oxidized product emits light in the presence of luminol or acridium esters
What is the role of glucose-6-phosphate dehydrogenase (G6PD) in immunoassay?
It is an enzyme label used in Microparticle Enzyme Immunoassay
What is the principle behind Enzyme Multiplied Immunoassay Technique (EMIT)?
Unlabeled analyte competes with enzyme-labeled analyte for antibody binding sites
What is the relationship between the rate of product formation and the analyte concentration in EMIT?
The rate of product formation is directly proportional to the analyte concentration
How does Microparticle Enzyme Immunoassay (MEIA) resemble ELISA?
Both use double-antibody system
What is the most accurate tool in the evaluation of thyroid nodules?
Fine Needle Aspirate Biopsy (FNAB)
What is the significance of early detection and treatment in neonates with thyroid hormone deficiency?
To eliminate severe mental retardation
Which of the following is the most sensitive test for neonatal screening of hypothyroidism?
TSH
What is the principle of Fluorescent Polarization Immunoassay (FPIA) in measuring thyroxine?
Competitive reaction: fluorophore-labeled thyroxine competes with patient thyroxine for antibody in a homogeneous system
What is the principle of Fluorescent Substrate-Labeled Inhibition Immunoassay in measuring thyroxine?
Competitive reaction: fluorogenic substrate-labeled thyroxine competes with patient T4 for antibody in a homogeneous system
What is the highest level of fluorescence emitted in Fluorescent Substrate-Labeled Inhibition Immunoassay?
By sample or standard with highest levels of T4
This quiz covers the diagnosis and evaluation of thyroid disorders, including thyroid nodules, Grave's disease, and neonatal hypothyroidism.
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