Thin Layer Chromatography (TLC) Stationary Phase and Solid Layers Preparation

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Questions and Answers

Which compound is commonly used as a binding agent in the preparation of thin layer chromatography stationary phases?

  • MgSO4
  • CaSO4 (correct)
  • NaCl
  • NaOH

In thin layer chromatography, what is the typical purpose of adding ammonia or diethyl amine in the separation of organic bases?

  • To neutralize the acidic group (correct)
  • To enhance adsorption
  • To increase resolution
  • To improve partitioning

Which stationary phase in thin layer chromatography has the highest resolution among the commonly used ones?

  • Kieselguhr
  • Alumina
  • Cellulose
  • Silica gel (correct)

What problem may arise when using binding agents like CaSO4 with silica gel in thin layer chromatography?

<p>Degradation of inorganic compounds (A)</p> Signup and view all the answers

Why is alumina not typically used with sensitive compounds in thin layer chromatography?

<p>Chemical reactivity causing degradation (A)</p> Signup and view all the answers

What type of chromatography is alumina primarily associated with in thin layer chromatography?

<p>Adsorption (A)</p> Signup and view all the answers

What is the function of glacial acid in separation of acidic compounds in Thin Layer Chromatography?

<p>Improve resolution power (C)</p> Signup and view all the answers

What is the main reason microcrystalline cellulose does not require a binder in Thin Layer Chromatography?

<p>Fibrous nature of cellulose (C)</p> Signup and view all the answers

Why is polyamide used in the separation of flavonoids in Thin Layer Chromatography?

<p>To improve resolution power (D)</p> Signup and view all the answers

What is an advantage of Thin Layer Chromatography over Partition Chromatography (PC) according to the given information?

<p>Rapid development (C)</p> Signup and view all the answers

How does mixing Kieselguhr with silica gel impact the adsorbent in Thin Layer Chromatography?

<p>Enhances adherence power (D)</p> Signup and view all the answers

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