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Thin Layer Chromatography (TLC) Stationary Phase and Solid Layers Preparation

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Thin Layer Chromatography (TLC) Stationary Phase and Solid Layers Preparation

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Questions and Answers

Which compound is commonly used as a binding agent in the preparation of thin layer chromatography stationary phases?

MgSO4

CaSO4 (correct)

NaCl

NaOH

In thin layer chromatography, what is the typical purpose of adding ammonia or diethyl amine in the separation of organic bases?

To neutralize the acidic group (correct)

To enhance adsorption

To increase resolution

To improve partitioning

Which stationary phase in thin layer chromatography has the highest resolution among the commonly used ones?

Kieselguhr

Alumina

Cellulose

Silica gel (correct)

What problem may arise when using binding agents like CaSO4 with silica gel in thin layer chromatography?

Degradation of inorganic compounds Signup and view all the answers

Why is alumina not typically used with sensitive compounds in thin layer chromatography?

Chemical reactivity causing degradation Signup and view all the answers

What type of chromatography is alumina primarily associated with in thin layer chromatography?

Adsorption Signup and view all the answers

What is the function of glacial acid in separation of acidic compounds in Thin Layer Chromatography?

Improve resolution power Signup and view all the answers

What is the main reason microcrystalline cellulose does not require a binder in Thin Layer Chromatography?

Fibrous nature of cellulose Signup and view all the answers

Why is polyamide used in the separation of flavonoids in Thin Layer Chromatography?

To improve resolution power Signup and view all the answers

What is an advantage of Thin Layer Chromatography over Partition Chromatography (PC) according to the given information?

Rapid development Signup and view all the answers

How does mixing Kieselguhr with silica gel impact the adsorbent in Thin Layer Chromatography?

Enhances adherence power Signup and view all the answers