Thin-Layer Chromatography vs High Performance Liquid Chromatography

The number of components in the mixture

By using lower temperatures for separation

To provide a potential difference for detecting ions

<p>Thin-layer chromatography uses a thin layer of sorbent coated on a plate, while high-performance liquid chromatography uses pressure and a column filled with absorbent material with small particle size.</p> Signup and view all the answers

<p>Pressure in HPLC is used for fast separations and to generate a high pressure up to 40 megaPascals.</p> Signup and view all the answers

<p>The stationary phase in HPLC is made up of an adsorbent material with very small particle size, providing a larger surface area for sample interactions.</p> Signup and view all the answers

<p>The flow rate of the mobile phase in HPLC is usually 1-3 mL/min.</p> Signup and view all the answers

<p>Chemically modified silica, divinely benzene, etc.</p> Signup and view all the answers

<p>The type of sample molecules to be separated.</p> Signup and view all the answers

<p>To detect the LUI of the sample coming out of the column.</p> Signup and view all the answers

<p>UV detector, IR detector, fluorescence detector, refractive index detector, mass spectrometer, electrochemical detector.</p> Signup and view all the answers