11 Questions
What does the number of peaks in a gas chromatogram indicate?
The number of components in the mixture
How does liquid chromatography achieve better separation of thermolabile compounds?
By using lower temperatures for separation
What is the purpose of using two electrodes in liquid chromatography?
To provide a potential difference for detecting ions
What is the difference between thin-layer chromatography and high-performance liquid chromatography?
Thin-layer chromatography uses a thin layer of sorbent coated on a plate, while high-performance liquid chromatography uses pressure and a column filled with absorbent material with small particle size.
What is the purpose of using pressure in high-performance liquid chromatography?
Pressure in HPLC is used for fast separations and to generate a high pressure up to 40 megaPascals.
How does the stationary phase in high-performance liquid chromatography differ from that in column chromatography?
The stationary phase in HPLC is made up of an adsorbent material with very small particle size, providing a larger surface area for sample interactions.
What is the typical flow rate of the mobile phase in high-performance liquid chromatography?
The flow rate of the mobile phase in HPLC is usually 1-3 mL/min.
What is used as the stationary phase in high-performance liquid chromatography (HPLC)?
Chemically modified silica, divinely benzene, etc.
What determines the choice of the mobile phase solvent in chromatography?
The type of sample molecules to be separated.
What is the purpose of the detector in high-performance liquid chromatography (HPLC)?
To detect the LUI of the sample coming out of the column.
Name one type of detector that can be used in high-performance liquid chromatography (HPLC).
UV detector, IR detector, fluorescence detector, refractive index detector, mass spectrometer, electrochemical detector.
Explore the differences between thin-layer chromatography and high performance liquid chromatography. Learn about the components, materials, and applications of both chromatography techniques.
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