24 Questions
What parameter has a significant role on the movement of the sample in the mobile phase?
pH
Which of the following factors is NOT mentioned as important for the quality of paper chromatography?
Flow rate of the mobile phase
Which of the following is the BEST way to increase the movement of the sample in paper chromatography?
Increase the pH of the mobile phase
What is the MOST important factor in determining the quality of paper chromatography results?
Purity of the adsorbent
Which of the following is the LEAST important factor in paper chromatography, according to the information provided?
pH of the mobile phase
What parameters are crucial for using TLC to monitor a chemical reaction?
Temperature and purity of mobile phase solvents
How does TLC help in determining the completion of a chemical reaction?
By comparing R₂ values of reactants with the reaction mixture
What role does the amount of loaded sample play in TLC analysis of a chemical reaction?
It affects separation efficiency
Which factor is NOT critical for using TLC to monitor a chemical reaction?
Length of the reaction vessel
What insight does comparing R₂ values provide when using TLC for chemical reaction monitoring?
Completion status of the reaction
What is the main factor affecting the shape of the spot in the given context?
Non-uniform spreading of the stationary phase on the support plate
Which of the following is NOT a consequence of non-uniform spreading of the stationary phase on the support plate?
Increased risk of sample degradation
What is the term used to describe the support plate in the given context?
Support plate
Which of the following statements is TRUE regarding the effect of non-uniform spreading of the stationary phase?
It leads to irregular spot shapes, affecting the accuracy of the analysis
What is the purpose of the stationary phase in the given context?
To interact with the analyte molecules and facilitate separation
How does the polarity of the stationary phase affect the interaction between solutes and the stationary phase?
It enhances the interaction
In reversed-phase TLC, what type of stationary phase is typically used?
Polar stationary phase
What is the primary difference between reversed-phase TLC and normal-phase TLC?
The polarity of the stationary phase
Which type of TLC utilizes a non-polar stationary phase?
Normal-phase TLC
What effect does a solid stationary phase have on the interaction between solutes and the stationary phase?
Strengthens the interaction
What factors can influence the distance travelled by the sample in chromatography?
Eluent strength and sample components
How can the sample components travel in chromatography?
In both straight and non-straight paths
What is the relationship between the polarity of the mobile phase and the distance travelled by the sample in chromatography?
Higher polarity of the mobile phase leads to greater distance travelled by the sample
Which of the following statements about the components of the sample in chromatography is correct?
The components of the sample may travel in both straight and non-straight paths
Learn about the factors that influence the Rf value in Thin Layer Chromatography (TLC), such as the type of stationary phase (solid or liquid) and its polarity. Understand how these factors impact the interaction between solutes and the stationary phase in reversed phase TLC or normal phase TLC.
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