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Questions and Answers
What is the principle on which chromatography works?
What is the principle on which chromatography works?
- Difference in boiling points of compounds
- Difference in color of compounds
- Difference in molecular weights of compounds
- Difference in solubilities and adsorption of compounds to two phases (correct)
What is the solvent generally used in Thin Layer Chromatography (TLC)?
What is the solvent generally used in Thin Layer Chromatography (TLC)?
- Organic liquid such as alcohol or ether (correct)
- Inorganic liquid such as sulfuric acid
- Water
- Acetone
What does adsorption refer to in chromatography?
What does adsorption refer to in chromatography?
- Evaporation of components from the stationary phase
- Adherence of components to the surface of the stationary phase (correct)
- Evaporation of components from the moving phase
- Adherence of components to the surface of the moving phase
Which substance is commonly used as the stationary phase in chromatography?
Which substance is commonly used as the stationary phase in chromatography?
What determines the amount that components adhere to the stationary phase in chromatography?
What determines the amount that components adhere to the stationary phase in chromatography?
Flashcards
Chromatography principle
Chromatography principle
Separation of substances based on differences in solubility and adsorption to two phases.
TLC solvent
TLC solvent
An organic liquid, like alcohol or ether, used in thin-layer chromatography.
Chromatography adsorption
Chromatography adsorption
Sticking of components to the stationary phase's surface.
Stationary phase
Stationary phase
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Adherence factor
Adherence factor
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Study Notes
Chromatography Principles
- Chromatography works on the principle of separation of components based on their differences in partitioning between a stationary phase and a mobile phase.
Thin Layer Chromatography (TLC)
- The solvent generally used in Thin Layer Chromatography (TLC) is a volatile organic solvent.
Chromatography Terminology
- Adsorption refers to the attraction and retention of molecules on the surface of the stationary phase.
Stationary Phase
- Silica gel is a commonly used substance as the stationary phase in chromatography.
Component Adhesion
- The amount that components adhere to the stationary phase in chromatography is determined by their intermolecular forces, such as van der Waals' forces and hydrogen bonding.
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Description
Test your knowledge of Thin Layer Chromatography with this quiz! Learn about the principles and processes involved in separating mixtures of compounds using this analytical technique.
