Questions and Answers
What is the principle on which chromatography works?
Difference in solubilities and adsorption of compounds to two phases
What is the solvent generally used in Thin Layer Chromatography (TLC)?
Organic liquid such as alcohol or ether
What does adsorption refer to in chromatography?
Adherence of components to the surface of the stationary phase
Which substance is commonly used as the stationary phase in chromatography?
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What determines the amount that components adhere to the stationary phase in chromatography?
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Study Notes
Chromatography Principles
- Chromatography works on the principle of separation of components based on their differences in partitioning between a stationary phase and a mobile phase.
Thin Layer Chromatography (TLC)
- The solvent generally used in Thin Layer Chromatography (TLC) is a volatile organic solvent.
Chromatography Terminology
- Adsorption refers to the attraction and retention of molecules on the surface of the stationary phase.
Stationary Phase
- Silica gel is a commonly used substance as the stationary phase in chromatography.
Component Adhesion
- The amount that components adhere to the stationary phase in chromatography is determined by their intermolecular forces, such as van der Waals' forces and hydrogen bonding.
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