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Thin Layer Chromatography Quiz

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Questions and Answers

What is the principle on which chromatography works?

Difference in boiling points of compounds

Difference in color of compounds

Difference in molecular weights of compounds

Difference in solubilities and adsorption of compounds to two phases (correct)

What is the solvent generally used in Thin Layer Chromatography (TLC)?

Organic liquid such as alcohol or ether (correct)

Inorganic liquid such as sulfuric acid

Water

Acetone

What does adsorption refer to in chromatography?

Evaporation of components from the stationary phase

Adherence of components to the surface of the stationary phase (correct)

Evaporation of components from the moving phase

Adherence of components to the surface of the moving phase

Which substance is commonly used as the stationary phase in chromatography?

<p>Silica gel (SiO2 x H2O)</p> Signup and view all the answers

What determines the amount that components adhere to the stationary phase in chromatography?

<p>The 'polar' nature of the molecule or its localized charge</p> Signup and view all the answers

Study Notes

Chromatography Principles

Chromatography works on the principle of separation of components based on their differences in partitioning between a stationary phase and a mobile phase.

Thin Layer Chromatography (TLC)

The solvent generally used in Thin Layer Chromatography (TLC) is a volatile organic solvent.

Chromatography Terminology

Adsorption refers to the attraction and retention of molecules on the surface of the stationary phase.

Stationary Phase

Silica gel is a commonly used substance as the stationary phase in chromatography.

Component Adhesion

The amount that components adhere to the stationary phase in chromatography is determined by their intermolecular forces, such as van der Waals' forces and hydrogen bonding.

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Description

Test your knowledge of Thin Layer Chromatography with this quiz! Learn about the principles and processes involved in separating mixtures of compounds using this analytical technique.