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Questions and Answers
What is the significance of a positive ΔH in a chemical reaction?
What is the significance of a positive ΔH in a chemical reaction?
Which statement accurately describes the relationship between entropy and spontaneity of reactions?
Which statement accurately describes the relationship between entropy and spontaneity of reactions?
What does ΔG⁰' represent in biochemical reactions?
What does ΔG⁰' represent in biochemical reactions?
What is ATP primarily used for in cellular processes?
What is ATP primarily used for in cellular processes?
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In coupled reactions, how do endergonic reactions derive energy?
In coupled reactions, how do endergonic reactions derive energy?
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Which type of biochemical reaction involves the transfer of electrons?
Which type of biochemical reaction involves the transfer of electrons?
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What occurs in a ligation reaction?
What occurs in a ligation reaction?
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Which of the following describes isomerization reactions?
Which of the following describes isomerization reactions?
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What role does lingual lipase play in lipid metabolism?
What role does lingual lipase play in lipid metabolism?
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Which statement accurately describes the function of bile acids in lipid digestion?
Which statement accurately describes the function of bile acids in lipid digestion?
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What is the primary function of chylomicrons in lipid transport?
What is the primary function of chylomicrons in lipid transport?
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What occurs to VLDL after it has delivered triglycerides to tissues?
What occurs to VLDL after it has delivered triglycerides to tissues?
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Which of the following correctly describes LDL?
Which of the following correctly describes LDL?
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Which lipoprotein is responsible for hydrolyzing triglycerides from chylomicrons?
Which lipoprotein is responsible for hydrolyzing triglycerides from chylomicrons?
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What is formed when monoglycerides and free fatty acids are taken up by intestinal cells?
What is formed when monoglycerides and free fatty acids are taken up by intestinal cells?
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What is the role of LDL receptors in lipid metabolism?
What is the role of LDL receptors in lipid metabolism?
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Which process occurs after bile acids emulsify dietary fats?
Which process occurs after bile acids emulsify dietary fats?
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Which is NOT a function of VLDL?
Which is NOT a function of VLDL?
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What is the first step in fatty acid synthesis catalyzed by Acetyl-CoA Carboxylase?
What is the first step in fatty acid synthesis catalyzed by Acetyl-CoA Carboxylase?
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What is the outcome of each cycle of β-oxidation of fatty acids?
What is the outcome of each cycle of β-oxidation of fatty acids?
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Where does the synthesis of triacylglycerols primarily occur?
Where does the synthesis of triacylglycerols primarily occur?
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Which enzyme is crucial for the final step of triacylglycerol synthesis?
Which enzyme is crucial for the final step of triacylglycerol synthesis?
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What is the role of the carnitine shuttle in fatty acid metabolism?
What is the role of the carnitine shuttle in fatty acid metabolism?
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What type of reaction occurs during the β-oxidation of fatty acids?
What type of reaction occurs during the β-oxidation of fatty acids?
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What happens to free fatty acids produced during lipolysis?
What happens to free fatty acids produced during lipolysis?
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What is the main energy source for the elongation of fatty acids in fatty acid synthesis?
What is the main energy source for the elongation of fatty acids in fatty acid synthesis?
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How long is the fatty acid chain typically extended before termination occurs during synthesis?
How long is the fatty acid chain typically extended before termination occurs during synthesis?
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What initiates the activation of fatty acids before β-oxidation?
What initiates the activation of fatty acids before β-oxidation?
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What is the primary purpose of transamination in amino acid catabolism?
What is the primary purpose of transamination in amino acid catabolism?
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Which enzyme is specifically involved in the oxidative deamination of glutamate?
Which enzyme is specifically involved in the oxidative deamination of glutamate?
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How is excess ammonia primarily transported in the bloodstream?
How is excess ammonia primarily transported in the bloodstream?
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What is the role of the alanine cycle during protein breakdown?
What is the role of the alanine cycle during protein breakdown?
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Why is it important to manage free ammonia produced during oxidative deamination?
Why is it important to manage free ammonia produced during oxidative deamination?
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Which metabolic pathway is primarily responsible for detoxifying ammonia in the liver?
Which metabolic pathway is primarily responsible for detoxifying ammonia in the liver?
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What is the basic function of aminotransferases in amino acid metabolism?
What is the basic function of aminotransferases in amino acid metabolism?
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During fasting, what is the significance of amino acid catabolism and nitrogen disposal?
During fasting, what is the significance of amino acid catabolism and nitrogen disposal?
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What happens to the carbon skeleton after the amino group is removed from the amino acid?
What happens to the carbon skeleton after the amino group is removed from the amino acid?
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In which tissue does the combination of pyruvate and ammonia to form alanine primarily occur?
In which tissue does the combination of pyruvate and ammonia to form alanine primarily occur?
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What is the primary purpose of the urea cycle in the body?
What is the primary purpose of the urea cycle in the body?
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Which enzyme requires N-acetylglutamate as an activator in the urea cycle?
Which enzyme requires N-acetylglutamate as an activator in the urea cycle?
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What potential consequence may arise from a genetic defect in any urea cycle enzyme?
What potential consequence may arise from a genetic defect in any urea cycle enzyme?
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In the urea cycle, what happens to fumarate after it is cleaved from argininosuccinate?
In the urea cycle, what happens to fumarate after it is cleaved from argininosuccinate?
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Which condition is characterized by elevated ammonia levels in the blood?
Which condition is characterized by elevated ammonia levels in the blood?
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Study Notes
Thermodynamics and Free Energy
- Endothermic Reactions: Reactions with a positive ΔH (enthalpy change) absorb heat from the surroundings.
- Entropy (S): A measure of the disorder or randomness of a system. Systems naturally tend towards higher entropy.
- Spontaneous Reactions: An increase in entropy favors the spontaneity of a reaction.
- Standard Free Energy Change (ΔG⁰'): Measured under specific conditions (1 M concentration of reactants/products, pH 7, 25°C, and 1 atm pressure).
- Actual Free Energy Change (ΔG): Depends on the concentration of reactants and products, which can vary within cells.
ATP - The Energy Currency
- Adenosine Triphosphate (ATP): The primary energy carrier in the cell.
- High-Energy Bonds: ATP stores energy in its high-energy phosphoanhydride bonds.
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Hydrolysis of ATP: Release of energy that drives biological reactions.
- ATP to ADP: Releases -30.5 kJ/mol of energy.
- ATP to AMP: Releases energy
- ATP Regeneration: Through processes like oxidative phosphorylation and glycolysis.
Coupled Reactions
- Endergonic Reactions: Reactions with a positive ΔG (free energy change).
- Exergonic Reactions: Reactions with a negative ΔG.
- Coupled Reactions: Endergonic reactions can be driven forward by coupling them to exergonic reactions, often ATP hydrolysis.
Types of Biochemical Reactions
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Oxidation-Reduction (Redox) Reactions: Involve the transfer of electrons.
- Oxidation: Loss of electrons.
- Reduction: Gain of electrons.
- Cellular Respiration: Electrons are transferred from nutrients (e.g., glucose) to oxygen, producing ATP.
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Ligation Reactions: Use ATP to form bonds between molecules.
- Example: Synthesis of oxaloacetate from pyruvate and carbon dioxide.
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Isomerization Reactions: Rearrangement of atoms within a molecule.
- Example: Conversion of glucose-6-phosphate to fructose-6-phosphate in glycolysis.
- Group Transfer Reactions: Transfer of a chemical group from one molecule to another.
Lipid Metabolism
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Digestion: Breakdown of dietary lipids for absorption.
- Mouth: Lingual lipase starts the process.
- Stomach: Gastric lipase continues digestion, but pancreatic lipase plays a larger role.
- Small Intestine: Bile acids emulsify dietary fats, increasing surface area. Pancreatic lipase hydrolyzes triglycerides into monoglycerides and free fatty acids.
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Absorption: Dietary lipids are absorbed by the intestinal mucosa.
- Micelle Formation: Digested lipids form micelles with bile salts.
- Enterocyte Uptake: Monoglycerides and free fatty acids are taken up by intestinal cells (enterocytes).
- Chylomicron Formation: Triglycerides are packaged into chylomicrons, which enter the lymphatic system and bloodstream.
Lipid Transport
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Chylomicrons: Transport dietary triglycerides and lipids from the intestine to peripheral tissues.
- Lipoprotein Lipase (LPL): Hydrolyzes triglycerides in chylomicrons, releasing fatty acids for uptake by tissues.
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Very Low-Density Lipoprotein (VLDL): Transports endogenous triglycerides from the liver to peripheral tissues.
- LPL: Similar to chylomicrons, VLDL triglycerides are hydrolyzed by LPL.
- Intermediate-Density Lipoprotein (IDL): Formed from VLDL after triglyceride removal. Can be converted into LDL or taken up by the liver.
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Low-Density Lipoprotein (LDL): Transports cholesterol to peripheral tissues.
- LDL Receptors: Peripheral tissues take up LDL through receptor-mediated endocytosis.
Fatty Acid Metabolism
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Synthesis: Occurs primarily in the cytoplasm of liver and adipose tissues.
- Starting Material: Acetyl-CoA, derived from carbohydrates and proteins.
- Key Enzyme: Acetyl-CoA Carboxylase (ACC) converts acetyl-CoA to malonyl-CoA.
- Fatty Acid Synthase Complex: Facilitates the elongation of the fatty acid chain by adding two-carbon units from malonyl-CoA.
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Process:
- Initiation: Acetyl-CoA and malonyl-CoA are loaded onto the synthase.
- Elongation: The fatty acid chain is extended by successive addition of two-carbon units.
- Termination: The process stops when the chain is 16 carbons (palmitate).
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Oxidation: Breakdown of fatty acids into acetyl-CoA units.
- Location: Mitochondria of cells.
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Steps:
- Activation: Fatty acids are activated to fatty acyl-CoA by acyl-CoA synthetase.
- Transport: Fatty acyl-CoA is transported into the mitochondria via the carnitine shuttle.
- β-Oxidation: In the mitochondria, fatty acyl-CoA undergoes a series of four reactions: oxidation, hydration, oxidation, and cleavage.
- Outcome: Each round of β-oxidation shortens the fatty acid chain by two carbons, producing one acetyl-CoA molecule.
Triacylglycerol (Triglyceride) Metabolism
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Synthesis: Occurs mainly in the liver and adipose tissue.
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Process:
- Glycerol-3-Phosphate Formation: Derived from glucose through glycolysis (in the liver) or from glycerol (in adipose tissue).
- Acylation: Fatty acids are esterified to glycerol-3-phosphate to form triacylglycerols.
- Key Enzyme: Diacylglycerol acyltransferase (DGAT) catalyzes the final step of triacylglycerol synthesis.
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Process:
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Breakdown: Occurs in adipose tissue.
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Process:
- Lipolysis: Breakdown of triacylglycerols into glycerol and free fatty acids by hormone-sensitive lipase (HSL).
- Release: Free fatty acids are released into the bloodstream and transported to tissues for oxidation.
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Process:
Nitrogen Disposal
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Amino Acid Catabolism: Breakdown of amino acids.
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Transamination: The amino group is transferred from an amino acid to α-ketoglutarate, forming glutamate.
- Catalyzed by: Aminotransferases (transaminases).
- Reversible: This process is essential for amino acid degradation, particularly in the liver and muscles.
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Oxidative Deamination: Glutamate is converted to α-ketoglutarate and ammonia (NH₃).
- Catalyzed by: Glutamate dehydrogenase.
- Ammonia Toxicity: Requires immediate management due to its toxicity.
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Transamination: The amino group is transferred from an amino acid to α-ketoglutarate, forming glutamate.
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Ammonia Transport:
- Glutamine Formation: Peripheral tissues, like muscles, convert ammonia to glutamine by combining it with glutamate.
- Alanine Cycle: In muscle tissue, pyruvate combines with ammonia to form alanine, which travels to the liver for urea production.
- Detoxification in the Liver: The liver converts ammonia into urea through the urea cycle.
The Urea Cycle (Ornithine Cycle)
- Location: Liver cells.
- Purpose: Detoxifies ammonia by converting it to urea for kidney excretion.
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Steps:
- Carbamoyl Phosphate Formation: Ammonia combines with CO₂ to form carbamoyl phosphate, catalyzed by carbamoyl phosphate synthetase I.
- Ornithine and Citrulline Production: Carbamoyl phosphate donates its carbamoyl group to ornithine, forming citrulline.
- Argininosuccinate Formation: Citrulline combines with aspartate, forming argininosuccinate.
- Cleavage to Arginine and Fumarate: Argininosuccinate is cleaved into arginine and fumarate, which can be used in the citric acid cycle.
- Formation of Urea: Arginine is hydrolyzed by arginase to release urea and regenerate ornithine.
- Regulation: The cycle is regulated by the availability of its substrates and the concentration of N-acetylglutamate, which activates carbamoyl phosphate synthetase I.
Clinical Implications
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Hyperammonemia: High ammonia levels in the blood, leading to symptoms such as confusion, tremors, and coma.
- Caused by: Liver disease or genetic defects in urea cycle enzymes.
- Inherited Urea Cycle Disorders: Genetic defects in any of the enzymes of the urea cycle.
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Description
Explore the concepts of thermodynamics and free energy in this quiz. Learn about endothermic reactions, entropy, and the significance of ATP as the cellular energy currency. Test your understanding of energy changes and spontaneous reactions in biological systems.
