Podcast
Questions and Answers
What is a clone?
What is a clone?
How can some plants be cloned?
How can some plants be cloned?
What is required to clone a gene?
What is required to clone a gene?
Who produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs?
Who produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs?
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Who discovered restriction endonucleases in E. coli in the late 1960's?
Who discovered restriction endonucleases in E. coli in the late 1960's?
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Study Notes
Cloning
- A clone is an exact genetic replica of an organism or a DNA sequence
Plant Cloning
- Some plants can be cloned through vegetative propagation, where a new plant grows from a part of the parent plant
Gene Cloning
- To clone a gene, the following is required:
- A DNA molecule containing the gene of interest
- A vector (e.g. plasmid) to carry the gene
- A host organism (e.g. bacterium) to express the gene
- Restriction endonucleases to cut the gene and vector
Frog Cloning
- In 1962, John Gurdon produced clones of identical frogs by transplanting nuclei from a single frog embryo to many enucleate eggs
Discovery of Restriction Endonucleases
- In the late 1960s, Werner Arber and Hamilton Smith discovered restriction endonucleases in E. coli, which revolutionized molecular biology
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Description
Test your knowledge of cloning in biology with this quiz. Explore the concept of cloning, from plant propagation to genetic cloning, and understand the processes involved in creating identical cells and organisms.
