Podcast
Questions and Answers
The Central Dogma states that DNA is used to make ______ is used to make Protein.
The Central Dogma states that DNA is used to make ______ is used to make Protein.
RNA
A ______ region acts as the template for the transcribed RNA, located downstream of the transcription start site.
A ______ region acts as the template for the transcribed RNA, located downstream of the transcription start site.
transcribed
The transcription start site is denoted as '+1' corresponding to the first ______ at the 5' end of the RNA product.
The transcription start site is denoted as '+1' corresponding to the first ______ at the 5' end of the RNA product.
nucleotide
Prokaryotes depend largely on ______ regulation, using repressor proteins to control gene expression.
Prokaryotes depend largely on ______ regulation, using repressor proteins to control gene expression.
Eukaryotes depend on ______ regulation of gene expression, but also regulate gene expression with transcription factors.
Eukaryotes depend on ______ regulation of gene expression, but also regulate gene expression with transcription factors.
Basal transcription factors bind to the ______ to recruit RNA polymerase.
Basal transcription factors bind to the ______ to recruit RNA polymerase.
Regulatory transcription factors including both activators and ______ can bind to proximal control elements upstream of the promoter.
Regulatory transcription factors including both activators and ______ can bind to proximal control elements upstream of the promoter.
Position-independent elements called ______ greatly increase transcription when bound by transcriptional activators.
Position-independent elements called ______ greatly increase transcription when bound by transcriptional activators.
The ______ domain is located upstream of the transcription start site.
The ______ domain is located upstream of the transcription start site.
The base positions denoted by negative numbers are located ______ of the transcription start site.
The base positions denoted by negative numbers are located ______ of the transcription start site.
For transcription, one strand of DNA acts as a template for the production of an RNA strand by the enzyme ______ polymerase.
For transcription, one strand of DNA acts as a template for the production of an RNA strand by the enzyme ______ polymerase.
The process of transcription occurs in stages: ______ - RNA polymerase interacts with the promoter at the TATA box to establish a transcription complex.
The process of transcription occurs in stages: ______ - RNA polymerase interacts with the promoter at the TATA box to establish a transcription complex.
Transcription in eukaryotes makes ______-mRNA that must be processed to mature mRNA for translation.
Transcription in eukaryotes makes ______-mRNA that must be processed to mature mRNA for translation.
A ______ cap and a 3' polyA tail both protect the mRNA, aid in export of mature mRNA to the cytoplasm, and improve translation efficiency by the ribosome.
A ______ cap and a 3' polyA tail both protect the mRNA, aid in export of mature mRNA to the cytoplasm, and improve translation efficiency by the ribosome.
Splicing removes ______, which are extra nucleotides in the transcribed region of genes, and ligates together exons to make a mature mRNA with a correct protein coding sequence.
Splicing removes ______, which are extra nucleotides in the transcribed region of genes, and ligates together exons to make a mature mRNA with a correct protein coding sequence.
MRNA has a protein coding sequence using ______ which are translated using tRNA.
MRNA has a protein coding sequence using ______ which are translated using tRNA.
Translation occurs in the ______, a large multi-protein complex that interacts with mRNA and enzymatically assembles polypeptides.
Translation occurs in the ______, a large multi-protein complex that interacts with mRNA and enzymatically assembles polypeptides.
During ______, a charged tRNA enters the A-site.
During ______, a charged tRNA enters the A-site.
The ______ tRNA exits at the E site and the process repeats until a stop codon lines up with the A-site and binds to a release factor, halting translation.
The ______ tRNA exits at the E site and the process repeats until a stop codon lines up with the A-site and binds to a release factor, halting translation.
Translate an mRNA using a ______ table, starting at the first 5' AUG.
Translate an mRNA using a ______ table, starting at the first 5' AUG.
