3 Questions
What is the main difference between λgal and λdgal particles?
λgal particles lack essential genes for phage tail and head synthesis.
What happens when head genes are not deleted in the production of λdgal particles?
Tails are not added to the filled head, and no viable particles are produced in the lysate
What determines the number of missing phage genes in λgal and λbio particles?
The position of the cuts that generated the particle
Study Notes
- λgal and λbio particles lack certain genes from the prophage.
- The number of missing phage genes depends on the position of the cuts that generated the particle.
- The missing phage genes come from the central region of the phage DNA.
- λgal particles are nonviable and defective, lacking essential genes for phage tail and head synthesis.
- λdgal particles contain cohesive ends and information for DNA replication and transcription.
- λdgal particles go through a normal life cycle, including bacterial lysis.
- If head genes are not deleted, the concatemeric branch produced by rolling circle replication is cleaved by the Ter system.
- Tails are not added to the filled head, and no viable particles are produced in the lysate.
- There is no discrepancy between the formation of a λdgal transducing particle and its ability to reproduce itself.
- λdgal particles fail to reproduce only because they lack the tail genes, but they arise from a normal prophage having a full set of genes.
Test your knowledge of λgal and λbio particles in this quiz! Learn about the missing phage genes in these particles and how they affect the particle's viability and ability to reproduce. Discover the differences between λgal and λdgal particles and their life cycles, including bacterial lysis. Challenge yourself to understand the role of head and tail genes and how they impact the production of viable particles in the lysate. Take this quiz to expand your knowledge of lambda phage particles and their genetic makeup.
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