Test Prioritization and Sample Examination Mastery Quiz

What is the recommended method for examining a skin biopsy sample?

Using routine staining with haematoxylin and eosin (H&E)
Using a microscope and knowing which magnification to use (correct)
Using tissue culture
Using immunohistochemistry

Which of the following tests can be requested in addition to routine staining for a skin biopsy sample?

Immunohistochemistry (correct)
Tissue culture for bacteria/fungi
Periodic Acid Schiff (PAS) for fungi
Ziehl-Neelsen (ZN) for mycobacteria

Under what circumstances is general anesthesia usually required for a skin biopsy?

For biopsies of the skin surface
For biopsies of feet, pinnae, lips, and noses (correct)
Only in calm animals
In all animals

What is the purpose of histopathology in skin biopsies?

To assess tissue structure (D) Signup and view all the answers

What is the recommended approach for taking a skin biopsy in calm animals?

Using sedation and local anesthesia (B) Signup and view all the answers

Which type of staining is routinely used for skin biopsies?

Haematoxylin and eosin (H&E) (B) Signup and view all the answers

What is the purpose of tissue culture in skin biopsies?

To culture and identify bacteria/fungi below skin surface (A) Signup and view all the answers

Which type of biopsy is quick and convenient, and should be used routinely with 6mm or 8mm biopsy punches?

Punch biopsy (D) Signup and view all the answers

When preparing the sample site for biopsy, what should be done with the crusts and skin surface?

Include them in the sample (C) Signup and view all the answers

What should be done if infiltrating local anesthetic for a biopsy?

Check efficacy of analgesia by pricking with a needle (A) Signup and view all the answers

What type of biopsy is recommended for excision of solitary nodules and biopsy of cutaneous masses?

Excisional biopsy (B) Signup and view all the answers

What should be done with the sample for histopathology after blotting blood from the underside?

Place it in sterile saline (A) Signup and view all the answers

What should be done if the sample examined is not representative of the lesion?

Ask the pathologist to cut further sections (A) Signup and view all the answers

Why do some biopsy results sometimes seem disappointing?

The sample was not representative of the lesion (A) Signup and view all the answers

What are biopsies essential for?

Diagnosing some skin diseases (C) Signup and view all the answers

What is the purpose of skin biopsies?

To establish a definitive diagnosis that cannot be reached by other, less invasive, testing methods and to rule out certain conditions. Signup and view all the answers

What tests are carried out on skin biopsies?

Histopathology and tissue culture. Signup and view all the answers

What is the role of histopathology in skin biopsies?

To assess tissue structure and provide additional information through special stains and immunohistochemistry. Signup and view all the answers

When is general anesthesia usually required for a skin biopsy?

For biopsies of feet, pinnae, lips, and noses in all animals. Signup and view all the answers

What is the recommended method for taking a skin biopsy in calm animals?

Sedation and local anesthesia. Signup and view all the answers

What is the importance of examining and interpreting the samples efficiently?

To obtain accurate results and be aware of the limitations of each test. Signup and view all the answers

Why is it necessary to take an adequate number of good quality diagnostic samples?

To ensure reliable and representative results. Signup and view all the answers

What are the best sites for taking a skin biopsy for alopecia and ulcerated skin lesions?

Across the margin of the alopecic area, the area of maximum hair loss, normal haired skin for alopecia. Skin just adjacent to the ulcer, where the epidermis is still intact for ulcerated skin lesions. Signup and view all the answers

What is the recommended method for preparing the sample site for biopsy?

Avoid disturbing the skin surface, clip hair but not too short, do not disturb crusts or skin surface, and do not prep or scrub the skin (unless excisional biopsy of nodules). Signup and view all the answers

What type of biopsy is quick and convenient, and should be used routinely with 6mm or 8mm biopsy punches?

Punch biopsy. Signup and view all the answers

What type of biopsy is recommended for excision of solitary nodules and biopsy of cutaneous masses?

Wedge/ellipse biopsy. Signup and view all the answers

How should a punch biopsy be performed to avoid destroying and shearing the sample?

Hold the punch perpendicular to the skin surface and rotate in one direction, not back and forth. Signup and view all the answers

How should a biopsy sample be handled for histopathology?

Handle gently by subcutaneous tissue only, never the dermis or epidermis, and use fine instruments or a needle. Signup and view all the answers

What should be done with the biopsy sample for histopathology after blotting blood from the underside?

Place the sample promptly into 10% formalin, using a minimum of 10 times the volume of the tissue sample. Signup and view all the answers

What are special considerations for bacterial and fungal tissue culture in skin biopsies?

Withdraw antibiotics and topical antimicrobials prior to sampling, submit punch biopsy sample in sterile saline, and avoid contamination with formalin or formalin fumes. Signup and view all the answers

Test Prioritization and Sample Examination Mastery Quiz

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Questions and Answers

What is the recommended method for examining a skin biopsy sample?

Which of the following tests can be requested in addition to routine staining for a skin biopsy sample?

Under what circumstances is general anesthesia usually required for a skin biopsy?

What is the purpose of histopathology in skin biopsies?

What is the recommended approach for taking a skin biopsy in calm animals?

Which type of staining is routinely used for skin biopsies?

What is the purpose of tissue culture in skin biopsies?

Which type of biopsy is quick and convenient, and should be used routinely with 6mm or 8mm biopsy punches?

When preparing the sample site for biopsy, what should be done with the crusts and skin surface?

What should be done if infiltrating local anesthetic for a biopsy?

What type of biopsy is recommended for excision of solitary nodules and biopsy of cutaneous masses?

What should be done with the sample for histopathology after blotting blood from the underside?

What should be done if the sample examined is not representative of the lesion?

Why do some biopsy results sometimes seem disappointing?

What are biopsies essential for?

What is the purpose of skin biopsies?

What tests are carried out on skin biopsies?

What is the role of histopathology in skin biopsies?

When is general anesthesia usually required for a skin biopsy?

What is the recommended method for taking a skin biopsy in calm animals?

What is the importance of examining and interpreting the samples efficiently?

Why is it necessary to take an adequate number of good quality diagnostic samples?

What are the best sites for taking a skin biopsy for alopecia and ulcerated skin lesions?

What is the recommended method for preparing the sample site for biopsy?

What type of biopsy is quick and convenient, and should be used routinely with 6mm or 8mm biopsy punches?

What type of biopsy is recommended for excision of solitary nodules and biopsy of cutaneous masses?

How should a punch biopsy be performed to avoid destroying and shearing the sample?

How should a biopsy sample be handled for histopathology?

What should be done with the biopsy sample for histopathology after blotting blood from the underside?

What are special considerations for bacterial and fungal tissue culture in skin biopsies?

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