Suhaimi Draman BSL3 Safety Protocol Quiz
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Questions and Answers

What precaution should be taken before touching the apparatus inside the BSC?

  • Do not pipette in/out too strong
  • Clean the floor if needed
  • Always wipe your hand with alcohol first (correct)
  • Wipe the wall of the BSC with tissue

    • How should you handle going out of BSL3 effectively?

  • Doff booties into the bin two at a time
  • Remove shoe covers one at a time while stepping into a 'clean zone' (correct)
  • Clean the floor if needed
  • Remove outer gloves and face mask first

    • What should be done after finishing work inside the BSC?

  • Set the incubator if needed
  • Take out the waste plastic bag and lay on surface and decon
  • Remove shoe covers one at a time
  • Clean the outer part of the bin inside out (correct)

    • What is one of the precautions mentioned when entering or exiting the BSC?

    <p>Wipe hand with alcohol after touching apparatus</p> Signup and view all the answers

    How should you handle waste disposal according to the text?

    <p>Label the waste date/name and store it for more than 24 hours</p> Signup and view all the answers

    What is recommended to do to minimize movement inside the BSC?

    <p>Pipette in and out slowly</p> Signup and view all the answers

    What should be done after entering the BSL3 laboratory?

    <p>Check the pressure gauges and switch on the BSC</p> Signup and view all the answers

    What is the purpose of wiping the prep desk with 70% alcohol?

    <p>To deactivate the virus or waste in waste beaker/bottle</p> Signup and view all the answers

    What should be done before bringing things from the passer box onto the clean area?

    <p>Wipe the prep desk with 70% alcohol</p> Signup and view all the answers

    What is the last step before starting work in the BSL3 laboratory?

    <p>Wipe one part of panel creating a clean area</p> Signup and view all the answers

    What should be done after wearing the suits before moving to the middle room?

    <p>Check if everything is okay</p> Signup and view all the answers

    What should be done after entering the middle room/airlock room?

    <p>Check if the door is closed properly and check the light</p> Signup and view all the answers

    What should you do upon arriving at the BSL3 area?

    <p>Check the pressure gauge for safety level</p> Signup and view all the answers

    What should you do if the pressure gauge in the back of the lab is not normal?

    <p>Alert others</p> Signup and view all the answers

    What should you do before entering the outer airlock of the BSL3 area?

    <p>Write name on whiteboard at the entrance door</p> Signup and view all the answers

    What is the correct order for donning safety gear before entering the BSL3 laboratory?

    <p>Invert the glove, tape it to Tyvek, wear N95 respirator</p> Signup and view all the answers

    What should you do if the pressure gauge in the outer airlock deviates from normal reading?

    <p>Do NOT ENTER BSL3 laboratory</p> Signup and view all the answers

    What should be done with all belongings/personal items upon entering the BSL3 area?

    <p>Place them in a basket on the table</p> Signup and view all the answers

    What should be done first when doffing the Tyvek suit?

    <p>Remove the booties and roll down the suit from the neck</p> Signup and view all the answers

    What item should be disinfected with 70% ethanol?

    <p>The goggles</p> Signup and view all the answers

    What is the correct way to remove the N95 respirator?

    <p>Remove head cap together with mask and inner gloves</p> Signup and view all the answers

    Where should personal items be taken after doffing gloves?

    <p>Anteroom</p> Signup and view all the answers

    What should be done after removing the name from the whiteboard at the entrance door?

    <p>Report any incidents in incident logbook if any</p> Signup and view all the answers

    What should be done before returning access cards/keys?

    <p>Log in if use</p> Signup and view all the answers

    Study Notes

    Laboratory Procedure

    • Wipe the apparatus and put it on a clean area in the Biosafety Cabinet (BSC)
    • Bring out clean apparatus to the tabletop
    • Wipe the working pad with alcohol and dispose of it in a waste plastic bin
    • Take out the waste plastic bag, lay it on a surface, and decontaminate it
    • Wipe the outer part of the bin from the bottom to the top and then inside
    • Decontaminate the surface and walls of the BSC inside and out
    • Let the BSC purge for a few minutes (~10 minutes)
    • Bring down the mirror and switch off the BSC

    Decontamination Precautions

    • Always wipe your hands with alcohol before touching apparatus
    • Be aware of clean and dirty areas
    • Minimize movement inside the BSC
    • Avoid pipetting too strongly and do it slowly
    • Avoid touching your face or mask
    • Wipe your hands with alcohol when entering and exiting the BSC

    Exiting BSL3 Laboratory

    • Label waste with date and name, and store it for more than 24 hours
    • Set the incubator if needed
    • Clean the floor if needed (monthly)
    • Place necessary items in the passer box
    • Doff booties one at a time while stepping into a "clean zone"
    • Remove outer gloves
    • Enter the airlock (next room)
    • Remove shoe covers one at a time while stepping into the "clean zone"
    • Check the mask fitness with a sucking air test
    • Enter the next room (middle room/airlock room)
    • Wear the second glove normally
    • Take goggles and wipe them with 70% ethanol
    • Last check for everything using the mirror
    • Enter the BSL3 laboratory

    Inside BSL3 Laboratory

    • Check the pressure gauges
    • Switch on the BSC, lift the glass door to the sash level, and let the blower ON for a few minutes
    • Prepare other required equipment like the centrifuge machine and computer
    • Refill the distilled water bottle
    • Wipe the prep desk with 70% alcohol
    • Bring things from the passer box to the clean area
    • Prepare necessary items like Absorbent pad, 70% alcohol spray, and Clorox for deactivating the virus
    • Wipe the BSC with a disinfectant
    • Divide the working area (clean/dirty/working space)
    • Spray hands with alcohol and start work

    Steps Going into BSL3

    • Alert others in WhatsApp group about checking in and working hours
    • Ensure specimens/samples are contained in sealable plastic bags and covered in a container
    • Check the surrounding area and pressure gauge for safety
    • Enter the autoclave room and switch on the autoclave if required
    • Check the whiteboard for reminders and log in to the entry and exit logbook
    • Check the sign on the door before entering the outer airlock

    Suit Up Procedure

    • Change into laboratory shoes/sandals
    • Place the basket containing the transfer box and materials at the side
    • Transfer the carried items to be used in BSL3 into the BSL3 passerbox
    • Cut the tape and put it on the wall
    • Gather items for suit-up on the desk
    • Don Tyvek, nitrile gloves, shoe covers, booties, mask, head cover, and N95 respirator
    • Ensure proper fit of the respirator

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    Description

    Test your knowledge on the safety protocol for entering a BSL3 area according to Suhaimi Draman's steps. Learn about checking in, handling specimens, and ensuring safety measures are in place before starting work.

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