Sterilization Methods Quiz
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Questions and Answers

Which of the following methods is NOT a means of achieving sterilization?

  • Disinfection (correct)
  • Filtration
  • Chemicals
  • Heat

    • What is the purpose of sterilizing surgical instruments and medications that enter an already aseptic part of the body?

  • To preserve foods for safe consumption
  • To reduce the entry of adventitious agents (correct)
  • To slow the decay of foods
  • To prevent food borne illness

    • What is the difference between sterilization and disinfection?

  • Sterilization and disinfection have no relation to each other.
  • Sterilization reduces all forms of life, while disinfection eliminates all forms of life.
  • Sterilization eliminates all forms of life, while disinfection reduces all forms of life. (correct)
  • Sterilization and disinfection are the same process.

    • Which temperature range is recommended for prion elimination in autoclave treatment?

    <p>134-138 °C</p> Signup and view all the answers

    Which strain of prion is inactivated relatively quickly by sterilization procedures?

    <p>263K scrapie prion</p> Signup and view all the answers

    What is the purpose of indicator tape in autoclaving?

    <p>To provide a visual confirmation of sterilization</p> Signup and view all the answers

    What is the standard setting for a hot air oven in terms of temperature and time for sterilization?

    <p>160 °C for 2 hours</p> Signup and view all the answers

    Which method of sterilization uses heated saturated steam under pressure to inactivate or kill microorganisms via denaturation of macromolecules?

    <p>Steam sterilization</p> Signup and view all the answers

    What is the formula for the number of microorganisms after sterilization time t, given the initial number N0 and the D-value?

    <p>$N = N_0 \cdot 10^{-t/D}$</p> Signup and view all the answers

    Which sterilization method is commonly used for heat- and moisture-sensitive medical devices?

    <p>Ethylene oxide gas sterilization</p> Signup and view all the answers

    What is the aim of sterilization?

    <p>To inactivate or kill microorganisms</p> Signup and view all the answers

    Which lenses in an Abbe condenser control the light that passes through the specimen before entering the objective lens on the microscope?

    <p>Both lenses</p> Signup and view all the answers

    What does the abbreviation 'DIN' stand for in the context of microscope objective lenses?

    <p>Deutsche Industrial Normen</p> Signup and view all the answers

    What is the purpose of retractable objective lenses in microscopes?

    <p>To protect the lens and the slide</p> Signup and view all the answers

    Which part of the microscope houses the eyepiece and objective lenses?

    <p>Head</p> Signup and view all the answers

    What is the function of the condenser lens in a microscope?

    <p>To focus the light that shines up through the slide</p> Signup and view all the answers

    What is the purpose of the rack stop in a microscope?

    <p>To determine how far up the stage can go</p> Signup and view all the answers

    What is the total magnification level when using a 10X eyepiece and a 40X objective lens?

    <p>400X</p> Signup and view all the answers

    Which type of microscope has two eyepiece lenses?

    <p>Binocular microscope</p> Signup and view all the answers

    What is the purpose of an Abbe condenser in a microscope?

    <p>To control the diameter and focal point of the beam of light entering the lens system</p> Signup and view all the answers

    What is the function of the arm in a microscope?

    <p>To connect the eyepiece tube to the base</p> Signup and view all the answers

    What is the purpose of a camera adapter in a microscope?

    <p>To enable a camera to fit on to the trinocular port of a microscope</p> Signup and view all the answers

    Which part of the microscope is responsible for concentrating the light on a specimen and increasing the resolution?

    <p>Condenser</p> Signup and view all the answers

    What is the function of a cover slip in microscopy?

    <p>To flatten out liquid samples</p> Signup and view all the answers

    Which type of microscope uses electrons rather than light to create an image of the target?

    <p>Electron microscope</p> Signup and view all the answers

    What determines the total magnification of a microscope?

    <p>The sum of the eyepiece magnification multiplied by that of the objective lens</p> Signup and view all the answers

    Which part of the microscope holds two or more objective lenses and can be rotated to easily change power?

    <p>Revolving Nosepiece</p> Signup and view all the answers

    What is the purpose of the stage clips on a microscope?

    <p>To hold the slides in place</p> Signup and view all the answers

    What is the total magnification when using a 40x objective lens and a 10x eyepiece lens?

    <p>400x</p> Signup and view all the answers

    What is the purpose of the illuminator on a microscope?

    <p>To provide a steady light source</p> Signup and view all the answers

    Which type of immersion oil is more viscous?

    <p>Type B</p> Signup and view all the answers

    What is the formula for calculating the total magnification of a microscope?

    <p>Total magnification = magnification of eyepiece lens × magnification of objective lens</p> Signup and view all the answers

    What is the purpose of the iris diaphragm on a microscope?

    <p>To control the illumination and adjust the contrast and resolution of a specimen</p> Signup and view all the answers

    What is the purpose of the rack stop on a microscope?

    <p>To prevent the objective lens from accidentally hitting the stage and damaging the specimen or slide</p> Signup and view all the answers

    Which part of the microscope is responsible for focusing the light on the specimen and adjusting the amount of light passing through?

    <p>Condenser</p> Signup and view all the answers

    What are the three structural parts of a microscope?

    <p>Head, base, and arm</p> Signup and view all the answers

    What are the optical parts of a microscope used for?

    <p>Magnifying and viewing the specimen</p> Signup and view all the answers

    Which part of a microscope is responsible for holding and supporting the microscope and its components?

    <p>Base</p> Signup and view all the answers

    What is the function of the optical parts of a microscope?

    <p>To view and magnify specimens</p> Signup and view all the answers

    Which component of a microscope is responsible for magnifying the specimen according to its focal strength?

    <p>Lens</p> Signup and view all the answers

    Which type of microscope uses lenses to refract visible light that passed through a thinly sectioned sample to produce an observable image?

    <p>Optical microscope</p> Signup and view all the answers

    When did the earliest known use of simple microscopes (magnifying glasses) date back to?

    <p>13th century</p> Signup and view all the answers

    What is the science of investigating small objects and structures using a microscope called?

    <p>Microscopy</p> Signup and view all the answers

    Who is credited with inventing the compound microscope?

    <p>Cornelis Drebbel</p> Signup and view all the answers

    When did Galileo Galilei build his own improved version of the compound microscope?

    <p>1624</p> Signup and view all the answers

    Who coined the name 'microscope' for the compound microscope?

    <p>Giovanni Faber</p> Signup and view all the answers

    Who achieved up to 300 times magnification using a simple single lens microscope?

    <p>Antonie van Leeuwenhoek</p> Signup and view all the answers

    Which type of microscope uses electrons rather than light to create an image of the target?

    <p>Transmission electron microscope</p> Signup and view all the answers

    What is the main purpose of fluorescence microscopy in biology?

    <p>To analyze cell structure at a molecular level</p> Signup and view all the answers

    Who developed the first practical confocal laser scanning microscope?

    <p>Thomas and Christoph Cremer</p> Signup and view all the answers

    Which microscope technique is approaching the resolution of electron microscopes?

    <p>Stimulated emission depletion (STED) microscopy</p> Signup and view all the answers

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