Sterilization Methods Quiz

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Questions and Answers

Which of these are examples of sterilization methods?

  • Syringe Filter (correct)
  • Incineration (correct)
  • Autoclaving (correct)
  • Ethanol (correct)
  • Heat (correct)

What kind of sterilization process is used for sterilization purposes in heat sensitive materials like vitamins and enzymes?

Mechanical

Which type of sterilization involves the use of ionizing radiation?

  • Syringe Filter
  • UV light (correct)
  • Autoclaving
  • Ethanol
  • X-ray (correct)
  • Heat
  • Incineration

What does the term 'moist heat sterilization' typically refer to?

<p>Autoclaving</p> Signup and view all the answers

Flashcards

Chemical Sterilization

Using chemicals to kill microorganisms or make them inactive.

Physical Sterilization

Using heat, radiation, or other physical methods to kill microorganisms or make them inactive.

Moist Autoclave

Uses high-pressure steam to sterilize.

Dry Oven Sterilization

Sterilization using high temperatures in a dry oven.

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Ionizing Radiation

Using radiation like X-rays or gamma rays to sterilize.

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Agar

A solidifying agent used in microbiology.

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Sterilization

Removal of all living organisms, including endospores, to create a sterile environment.

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Heat-Sensitive Solutions

Solutions that are damaged by high temperatures.

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Millipore filter

Used for filtering heat-sensitive solutions.

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Inoculation

Transferring an organism from one medium to another.

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Pour Plate Method

A method to create a bacterial culture using a sample of diluted organisms and a solid medium.

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Streak Method

A method of spreading a sample of microorganisms across a surface, allowing the individual colonies to grow.

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Incubation

the process of maintaining growth conditions to allow the growth of microorganisms.

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Growth Factors

The factors necessary for the growth of a microorganism.

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Media

A nutrient-rich environment that allows microorganisms to grow and support the growth.

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Study Notes

Chemical Sterilization

  • Examples include alcohol, Dettol, and disinfectants
  • These methods use chemical agents to kill microorganisms.

Physical Sterilization

  • Heat sterilisation, autoclave, moist heat, dry heat, incineration, and UV

Mechanical Sterilization

  • Used for heat-sensitive solutions including vitamins,
  • Enzymes, antibiotics, and vaccines.

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