Sterilization and Disinfection Methods Quiz
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Questions and Answers

What is the main function of ligase in DNA?

  • Assists in gluing DNA fragments together (correct)
  • Aids in the repair of DNA damage
  • Helps remove DNA fragments
  • Promotes DNA replication
  • Which process involves the transfer of genetic material from a donor cell to a recipient cell using sex pili?

  • Recombination
  • Transformation
  • Conjugation (correct)
  • Transduction
  • What mutation refers to a change in the original nucleotide sequence of a gene?

  • Transformation mutation
  • Recombination mutation
  • Genotypic mutation
  • Phenotypic mutation (correct)
  • Which type of organism requires oxygen to grow and thrives well in room air?

    <p>Facultative anaerobe</p> Signup and view all the answers

    What is the main difference between respiration and fermentation in energy production?

    <p>Respiration requires oxygen while fermentation does not.</p> Signup and view all the answers

    What is the primary characteristic of obligate anaerobes among anaerobic organisms?

    <p>They cannot grow in the presence of oxygen.</p> Signup and view all the answers

    What can cause goiter, hypothyroidism, and other issues when the skin is immunocompromised?

    <p>Iodophor</p> Signup and view all the answers

    Which element is found in spores and can affect the degree of killing of organisms?

    <p>Calcium</p> Signup and view all the answers

    Which substance is no longer used for ophthalmia neonatorum?

    <p>Silver nitrate</p> Signup and view all the answers

    What is one of the factors that affects the exposure time needed for 99.9% elimination of organisms?

    <p>The presence of spores</p> Signup and view all the answers

    Which type of organism is most resistant to heat, chemicals, and radiation?

    <p>Prions</p> Signup and view all the answers

    What determines the exposure time necessary for 99.9% elimination of organisms?

    <p>The microbial load</p> Signup and view all the answers

    What is the highest temperature range for incineration as a physical method of sterilization?

    <p>900 C - 1000 C</p> Signup and view all the answers

    Which method is classified as a chemical method of sterilization?

    <p>Ethylene oxide</p> Signup and view all the answers

    What is the temperature requirement for moist heat sterilization via autoclave?

    <p>121 C at 1 atm</p> Signup and view all the answers

    Which type of filter is used in air filtration for sterilization?

    <p>High efficiency particulate air (HEPA) filter</p> Signup and view all the answers

    What effect does ionizing radiation have on DNA during sterilization?

    <p>Destroy DNA through mutation</p> Signup and view all the answers

    What is the main disadvantage of dry heat sterilization compared to moist heat?

    <p>Longer exposure time</p> Signup and view all the answers

    What is the main difference between exotoxins and endotoxins?

    <p>Release mechanism</p> Signup and view all the answers

    What does a capsule around bacteria help resist?

    <p>Phagocytosis</p> Signup and view all the answers

    Which toxin is produced by Clostridium botulinum?

    <p>Neurotoxins</p> Signup and view all the answers

    When are specimens ideally collected for testing?

    <p>Before the medicines are administered</p> Signup and view all the answers

    Which factor causes tissue and cellular damage in pathogenic microorganisms?

    <p>Hyaluronidase</p> Signup and view all the answers

    What is the primary mode of transmission for Mycobacterium tuberculosis?

    <p>Through aerosols</p> Signup and view all the answers

    Which level of biocontainment deals with agents like Congo-Crimean hemorrhagic fever and Marburg virus?

    <p>Level 4</p> Signup and view all the answers

    What characterizes an opportunistic pathogen?

    <p>Causes disease when the host is immunocompromised</p> Signup and view all the answers

    What distinguishes a true pathogen from an opportunistic pathogen?

    <p>Ability to invade the tissue of healthy individuals</p> Signup and view all the answers

    Which type of infection originates from medical instruments or procedures?

    <p>Iatrogenic infection</p> Signup and view all the answers

    In the context of pathogenicity, what does Nosocomial pathogen refer to?

    <p>Hospital-acquired infections</p> Signup and view all the answers

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