Sterilization and Disinfection Methods Quiz
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Questions and Answers

What is the main function of ligase in DNA?

  • Assists in gluing DNA fragments together (correct)
  • Aids in the repair of DNA damage
  • Helps remove DNA fragments
  • Promotes DNA replication

Which process involves the transfer of genetic material from a donor cell to a recipient cell using sex pili?

  • Recombination
  • Transformation
  • Conjugation (correct)
  • Transduction

What mutation refers to a change in the original nucleotide sequence of a gene?

  • Transformation mutation
  • Recombination mutation
  • Genotypic mutation
  • Phenotypic mutation (correct)

Which type of organism requires oxygen to grow and thrives well in room air?

<p>Facultative anaerobe (C)</p> Signup and view all the answers

What is the main difference between respiration and fermentation in energy production?

<p>Respiration requires oxygen while fermentation does not. (A)</p> Signup and view all the answers

What is the primary characteristic of obligate anaerobes among anaerobic organisms?

<p>They cannot grow in the presence of oxygen. (B)</p> Signup and view all the answers

What can cause goiter, hypothyroidism, and other issues when the skin is immunocompromised?

<p>Iodophor (C)</p> Signup and view all the answers

Which element is found in spores and can affect the degree of killing of organisms?

<p>Calcium (C)</p> Signup and view all the answers

Which substance is no longer used for ophthalmia neonatorum?

<p>Silver nitrate (A)</p> Signup and view all the answers

What is one of the factors that affects the exposure time needed for 99.9% elimination of organisms?

<p>The presence of spores (C)</p> Signup and view all the answers

Which type of organism is most resistant to heat, chemicals, and radiation?

<p>Prions (A)</p> Signup and view all the answers

What determines the exposure time necessary for 99.9% elimination of organisms?

<p>The microbial load (A)</p> Signup and view all the answers

What is the highest temperature range for incineration as a physical method of sterilization?

<p>900 C - 1000 C (A)</p> Signup and view all the answers

Which method is classified as a chemical method of sterilization?

<p>Ethylene oxide (B)</p> Signup and view all the answers

What is the temperature requirement for moist heat sterilization via autoclave?

<p>121 C at 1 atm (B)</p> Signup and view all the answers

Which type of filter is used in air filtration for sterilization?

<p>High efficiency particulate air (HEPA) filter (B)</p> Signup and view all the answers

What effect does ionizing radiation have on DNA during sterilization?

<p>Destroy DNA through mutation (D)</p> Signup and view all the answers

What is the main disadvantage of dry heat sterilization compared to moist heat?

<p>Longer exposure time (C)</p> Signup and view all the answers

What is the main difference between exotoxins and endotoxins?

<p>Release mechanism (D)</p> Signup and view all the answers

What does a capsule around bacteria help resist?

<p>Phagocytosis (A)</p> Signup and view all the answers

Which toxin is produced by Clostridium botulinum?

<p>Neurotoxins (A)</p> Signup and view all the answers

When are specimens ideally collected for testing?

<p>Before the medicines are administered (B)</p> Signup and view all the answers

Which factor causes tissue and cellular damage in pathogenic microorganisms?

<p>Hyaluronidase (A)</p> Signup and view all the answers

What is the primary mode of transmission for Mycobacterium tuberculosis?

<p>Through aerosols (B)</p> Signup and view all the answers

Which level of biocontainment deals with agents like Congo-Crimean hemorrhagic fever and Marburg virus?

<p>Level 4 (C)</p> Signup and view all the answers

What characterizes an opportunistic pathogen?

<p>Causes disease when the host is immunocompromised (B)</p> Signup and view all the answers

What distinguishes a true pathogen from an opportunistic pathogen?

<p>Ability to invade the tissue of healthy individuals (A)</p> Signup and view all the answers

Which type of infection originates from medical instruments or procedures?

<p>Iatrogenic infection (B)</p> Signup and view all the answers

In the context of pathogenicity, what does Nosocomial pathogen refer to?

<p>Hospital-acquired infections (B)</p> Signup and view all the answers

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